Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey

İNAN BEKTAŞ, KADRİYE; BELDUZ, Ali Osman; CANAKCI, Sabriye

Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey

KADRİYE İNAN BEKTAŞ, (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Sabriye ÇANAKÇI, (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Ali Osman BELDÜZ (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Turkish Journal of Biology

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Yıl: 2011 Cilt: 35 Sayı: 5 Sayfa Aralığı: 529 - 542 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey

Öz:

Türkiye’nin batısında bulunan bazı kaplıcalarda, ksilan kullanan termofi lik mikroorganizmaların varlığı araştırıldı. Fenotipik özelliklerine ve 16S rRNA gen dizi analizi verilerine dayanarak, kaplıcalardan izole edilen ksilan kullanan 9 izolatın Anoxybacillus cinsine ait oldukları ve optimum 50-60 °C üreyebildikleri belirlendi. 16S rRNA gen dizi analizine göre, bu izolatların Anoxybacillus türlerine ≥97’den daha fazla bir benzerlik gösterdiği ve bu izolatların Anoxybacillus cinsine ait oldukları belirlendi. Yapılan DNA-DNA hibridizasyonu, SDS-PAGE analizi, biyokimyasal ve fi zyolojik özelliklere dayanarak, I3, CT1Sari ve BT2.1 izolatlarının Anoxybacillus gonensis’e; I4.2 ve B9.3 izolatlarının A. voinovskiensis’e; I4.1, AC26, ACT14 ve ACT2Sari izolatlarının da A. kestanbolensis’e ait yeni suşlar olduklarına karar verildi. İzolatların ksilan aktiveteleri, ksilanazların optimum sıcaklık, optimum pH ve pH kararlılıkları araştırıldı. ACT2Sari ve ACT14 izolatlarının ksilanazları, en yüksek optimum sıcaklığa (75 °C) sahipken; I3, CT1Sari, BT2.1, ACT2Sari ve ACT14 izolatlarının ksilanazları, en yüksek optimum pH’ya (pH 9,0) sahiptirler. I3, CT1Sari, BT2.1, I4.1, ACT2Sari, AC26 ve ACT14 izolatlarının ürettiği ksilanazlarının, hem yüksek sıcaklıkta hem de alkali pH’larda optimum aktiviteye sahip oldukları ve ayrıca I4.1, ACT2Sari, AC26 and ACT14 izolatlarının ksilanazlarının da, alkalin pH’larda yüksek kararlılık gösterdikleri belirlendi.

Anahtar Kelime: Türkiye termal kaynaklar kaynak suyu enzim aktivitesi Anoxybacillus karakterizasyon izolasyon ksilanolitik mikroorganizmalar DNA melezlemesi

Konular: Biyoloji

Türkiye’nin bazı kaplıcalarından ksilan kullanan yeni Anoxybacillus suşlarının izolasyonu ve karakterizasyonu

Öz:

Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species ≥97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profi le, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 isolates are new strains of A. voinovskiensis; and I4.1, AC26, ACT14, and ACT2Sari isolates are new strains of A. kestanbolensis. The presence of xylanase activities, their optimum temperature, pH stability, and optimum pH were also investigated. Th e isolates ACT2Sari and ACT14 had the highest temperature optima (75 °C), and I3, CT1Sari, BT2.1, ACT2Sari, and ACT14 had the highest pH optima (pH 9.0) of xylanase. Th e xylanases of I3, CT1Sari, BT2.1, I4.1, ACT2Sari, AC26, and ACT14 were optimally active both at alkaline pH and elevated temperature, and xylanases of I4.1, ACT2Sari, AC26, and ACT14 also were stable at alkaline pH.

Anahtar Kelime: Turkey thermal springs springs (water) enzyme activity Anoxybacillus characterization isolation xylanolytic microorganisms DNA hybridization

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	İNAN BEKTAŞ K, CANAKCI S, BELDUZ A (2011). Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. , 529 - 542.
Chicago	İNAN BEKTAŞ KADRİYE,CANAKCI Sabriye,BELDUZ Ali Osman Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. (2011): 529 - 542.
MLA	İNAN BEKTAŞ KADRİYE,CANAKCI Sabriye,BELDUZ Ali Osman Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. , 2011, ss.529 - 542.
AMA	İNAN BEKTAŞ K,CANAKCI S,BELDUZ A Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. . 2011; 529 - 542.
Vancouver	İNAN BEKTAŞ K,CANAKCI S,BELDUZ A Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. . 2011; 529 - 542.
IEEE	İNAN BEKTAŞ K,CANAKCI S,BELDUZ A "Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey." , ss.529 - 542, 2011.
ISNAD	İNAN BEKTAŞ, KADRİYE vd. "Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey". (2011), 529-542.

APA	İNAN BEKTAŞ K, CANAKCI S, BELDUZ A (2011). Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turkish Journal of Biology, 35(5), 529 - 542.
Chicago	İNAN BEKTAŞ KADRİYE,CANAKCI Sabriye,BELDUZ Ali Osman Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turkish Journal of Biology 35, no.5 (2011): 529 - 542.
MLA	İNAN BEKTAŞ KADRİYE,CANAKCI Sabriye,BELDUZ Ali Osman Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turkish Journal of Biology, vol.35, no.5, 2011, ss.529 - 542.
AMA	İNAN BEKTAŞ K,CANAKCI S,BELDUZ A Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turkish Journal of Biology. 2011; 35(5): 529 - 542.
Vancouver	İNAN BEKTAŞ K,CANAKCI S,BELDUZ A Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey. Turkish Journal of Biology. 2011; 35(5): 529 - 542.
IEEE	İNAN BEKTAŞ K,CANAKCI S,BELDUZ A "Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey." Turkish Journal of Biology, 35, ss.529 - 542, 2011.
ISNAD	İNAN BEKTAŞ, KADRİYE vd. "Isolation and characterization of xylanolytic new strains of Anoxybacillus from some hot springs in Turkey". Turkish Journal of Biology 35/5 (2011), 529-542.