A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate

NAIR, Jayakumaran; RAJAN, Akhila

A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate

Akhila RAJAN, (Department of Biotechnology, Kerala University, Kariavattom, Trivandrum 695 581, India)

Jayakumaran NAIR (Department of Biotechnology, Kerala University, Kariavattom, Trivandrum 695 581, India)

Turkish Journal of Biology

3 0

Yıl: 2011 Cilt: 35 Sayı: 5 Sayfa Aralığı: 569 - 574 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate

Öz:

A comparative study of solid-state fermentation (SSF) and submerged fermentation (SmF) for the production of alkaline lipase from a new isolate, Aspergillus fumigatus MTCC 9657, using defatted rice bran (DFRB) as a substrate was studied. Different process parameters, such as incubation period, initial pH and incubation temperature, were optimised to achieve the maximum yield of the alkaline lipase. Maximum enzyme production (8.13 U/mL) was obtained by day 7 of incubation in SSF compared to day 4 in SmF at an optimum pH of 8.5 and ambient temperature of 30 °C. Lipase produced by SSF was stable over a period of 15 days, whereas lipase production in SmF decreased by day 5. Lipase production in SSF with DFRB using sterile water as a moisture source exhibited more activity (577.5 U/70 mL) than that supplemented with mineral medium. Compared to the cost of culture medium, the solid-state substrate DFRB is inexpensive, and therefore this process is industrially and economically feasible. These results confi rm the interesting potential of SSF in DFRB without any additional nutritional supplement.

Anahtar Kelime: comparisons production rice bran fermentation economic importance Aspergillus fumigatus alkaline lipase

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	RAJAN A, NAIR J (2011). A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. , 569 - 574.
Chicago	RAJAN Akhila,NAIR Jayakumaran A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. (2011): 569 - 574.
MLA	RAJAN Akhila,NAIR Jayakumaran A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. , 2011, ss.569 - 574.
AMA	RAJAN A,NAIR J A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. . 2011; 569 - 574.
Vancouver	RAJAN A,NAIR J A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. . 2011; 569 - 574.
IEEE	RAJAN A,NAIR J "A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate." , ss.569 - 574, 2011.
ISNAD	RAJAN, Akhila - NAIR, Jayakumaran. "A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate". (2011), 569-574.

APA	RAJAN A, NAIR J (2011). A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turkish Journal of Biology, 35(5), 569 - 574.
Chicago	RAJAN Akhila,NAIR Jayakumaran A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turkish Journal of Biology 35, no.5 (2011): 569 - 574.
MLA	RAJAN Akhila,NAIR Jayakumaran A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turkish Journal of Biology, vol.35, no.5, 2011, ss.569 - 574.
AMA	RAJAN A,NAIR J A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turkish Journal of Biology. 2011; 35(5): 569 - 574.
Vancouver	RAJAN A,NAIR J A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turkish Journal of Biology. 2011; 35(5): 569 - 574.
IEEE	RAJAN A,NAIR J "A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate." Turkish Journal of Biology, 35, ss.569 - 574, 2011.
ISNAD	RAJAN, Akhila - NAIR, Jayakumaran. "A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate". Turkish Journal of Biology 35/5 (2011), 569-574.