Yıl: 2011 Cilt: 35 Sayı: 5 Sayfa Aralığı: 601 - 610 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon

Öz:
Sürgün ucu eksplantları kullanılarak Diyarbakır karpuz tipleri Sürme, Beyazkış ve Karakış’ın mikroçoğaltımı için hızlı bir protokol geliştirilmiştir. İn vitro koşullarda çimlendirilmiş 5 günlük fi delerin sürgün uçları 3 haft a süreyle sürgün rejenerasyon ortamında kültüre alınmıştır. Farklı konsantrasyonlardaki benzil adenin (BA) ve karbonhidrat tiplerinin sürgün proliferasyonuna etkileri test edilmiştir. Sonuçlar her 3 genotipte de 0,5-1,0 mg/L aralığındaki BA ortamında sürgün uzunluklarının hemen hemen eşit olduğunu göstermiş ve BA ortamına sukroz eklenmesiyle ortalama sürgün sayısı ve sürgün uzunluğunun diğer karbonhidratlara göre daha yüksek olduğu tespit edilmiştir. Her 3 genotip için en iyi köklenme ortamının 1.0 mg/L IBA olduğu belirlenmiş ve IBA’da köklendirilen sürgünler Beyazkış ve Karakış için % 85, Sürme için % 90 başarıyla steril komposta aktarılmıştır. Sürme genotipinde hem doğrudan tohumdan hem de aktarılmış fi delerden elde edilen ürün verimi ve ortalama meyve ağırlığı, Beyazkış ve Karakış’tan daha yüksek bulunmuştur. Temel çözünür şekerler HPLC (yüksek performanslı sıvı kromatografi ) ile kantitatif olarak tespit edilmiştir. Beyazkış’ta tohumdan elde edilen ve Karakış’ta aktarılmış fideden elde edilen karpuzda, en çok bulunan şeker fruktoz olarak tespit edilmiştir. Bu sonuçlarla yüksek kaliteli Diyarbakır karpuzunun in vitro çoğaltılabileceği gösterilmiştir.
Anahtar Kelime: verim şeker miktarı bitki büyüme düzenleyicileri karpuz doku kültürü meyveler iklime uyum in vitro yenilenme Diyarbakır

Konular: Biyoloji

Diyarbakır karpuzunun in vitro çoğaltılması ve doğrudan tohumdan ve aktarılmış fidelerden elde edilen karpuzların karşılaştırılması

Öz:
A rapid protocol using shoot tip explants for micropropagation of Diyarbakır watermelon [Citrullus lanatus (Th unb.) Matsum. & Nakai] types Sürme, Beyazkış and Karakış was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. The effects of the different concentrations of benzyl adenine (BA) and carbohydrate types on shoot proliferation were examined. Th e results confi rmed that a range of 0.5-1.0 mg/L of BA was almost equally effective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 genotypes studied in terms of the number of proliferated shoots and the average shoot length obtained. The highest percentage of rooting was achieved when medium supplemented with 1.0 mg/L of indole-3-butyric acid (IBA) was used for the 3 genotypes. The highest frequency of acclimatized plantlets for the 3 genotypes was 85%, 85%, and 90%, respectively, in sterile compost, when the shoots of Beyazkış, Karakış, and Sürme were rooted in the IBA-supplemented rooting treatments. The Sürme genotype produced a signifi cantly higher yield and mean fruit weight than the Beyazkış and Karakış genotypes in both direct-seeded and transplanted watermelons. The main soluble sugar of the experimental types was separated, identifi ed, and quantifi ed using high performance liquid chromatography (HPLC). Fructose was found to be the most abundant sugar and was highly detected in Beyazkış for the seeded watermelon and in Karakış for the transplanted watermelon. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakır watermelon for use in breeding lines.
Anahtar Kelime: acclimatization in vitro regeneration Diyarbakir yields sugar content plant growth regulators watermelons tissue culture fruits

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Okumuş V, PİRİNÇ V, ONAY A, BAŞARAN D (2011). In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. , 601 - 610.
Chicago Okumuş Veysi,PİRİNÇ Vedat,ONAY Ahmet,BAŞARAN Davut In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. (2011): 601 - 610.
MLA Okumuş Veysi,PİRİNÇ Vedat,ONAY Ahmet,BAŞARAN Davut In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. , 2011, ss.601 - 610.
AMA Okumuş V,PİRİNÇ V,ONAY A,BAŞARAN D In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. . 2011; 601 - 610.
Vancouver Okumuş V,PİRİNÇ V,ONAY A,BAŞARAN D In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. . 2011; 601 - 610.
IEEE Okumuş V,PİRİNÇ V,ONAY A,BAŞARAN D "In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon." , ss.601 - 610, 2011.
ISNAD Okumuş, Veysi vd. "In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon". (2011), 601-610.
APA Okumuş V, PİRİNÇ V, ONAY A, BAŞARAN D (2011). In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. Turkish Journal of Biology, 35(5), 601 - 610.
Chicago Okumuş Veysi,PİRİNÇ Vedat,ONAY Ahmet,BAŞARAN Davut In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. Turkish Journal of Biology 35, no.5 (2011): 601 - 610.
MLA Okumuş Veysi,PİRİNÇ Vedat,ONAY Ahmet,BAŞARAN Davut In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. Turkish Journal of Biology, vol.35, no.5, 2011, ss.601 - 610.
AMA Okumuş V,PİRİNÇ V,ONAY A,BAŞARAN D In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. Turkish Journal of Biology. 2011; 35(5): 601 - 610.
Vancouver Okumuş V,PİRİNÇ V,ONAY A,BAŞARAN D In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon. Turkish Journal of Biology. 2011; 35(5): 601 - 610.
IEEE Okumuş V,PİRİNÇ V,ONAY A,BAŞARAN D "In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon." Turkish Journal of Biology, 35, ss.601 - 610, 2011.
ISNAD Okumuş, Veysi vd. "In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon". Turkish Journal of Biology 35/5 (2011), 601-610.