Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury

AKYOL, MEHMET EDIP; SANCAK, TUNAHAN; okulmuş, Çağlar; KELES, OMER FARUK; CETİN, EYUP

Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury

Tunahan SANCAK, (Sivas Cumhuriyet Üniversitesi, Veteriner Fakültesi, Cerrahi Anabilim Dalı, Sivas, Türkiye)

Çağlar OKULMUŞ, (İzmir/Bornova Veteriner Kontrol Enstitüsü, Biyokimya Anabilim Dalı, İzmir, Türkiye)

Mehmet Edip AKYOL, (Van Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Beyin Cerrahi Anabilim Dalı, Van, Türkiye)

Ömer Faruk KELEŞ, (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Van, Türkiye)

Eyüp ÇETİN (Sağlık Bilimleri Üniversitesi, Haydarpaşa Eğitim ve Araştırma Hastanesi, Beyin Cerrahi Kliniği, İstanbul, Türkiye)

Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi

30 5

Yıl: 2023 Cilt: 37 Sayı: 3 Sayfa Aralığı: 230 - 236 Metin Dili: İngilizce İndeks Tarihi: 28-10-2023

Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury

Öz:

Objective: In this study, it was aimed to determine the effect of shilajit on the liver and kidney in rats with experimental spinal cord injury. Materials and Methods: Spinal cord injury-induced rats were treated with shilajit at doses of 150 mg/kg and 250 mg/kg on days 1, 2, and 3. Following the administration, rats were sacrificed on the 14th day, and blood, liver, and kidney tissues were collected. Results: The histopathological analysis of the liver and kidney revealed that high doses of shilajit showed a greater protective effect in the tissues and low doses showed only a partial protective effect. In biochemical analysis, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), creatine kinase (CK), creatine, and urea concentrations were lower and albumin (ALB) and total protein (TP) concentrations were higher in shilajit-treated groups compared with the control group (P range: 0.05–0.001). Conclusion: As a result, in this study, it has been histopathologically and biochemically revealed that administration of the Shilajit significantly suppressed the formation of the lesions in liver and kidney with its anti-inflammatory properties after spinal cord injury in rats.

Anahtar Kelime: Kidney liver spinal cord injury shilajit

Deneysel omurilik hasarı oluşturulan ratlarda shilajitin karaciğer ve böbrek üzerine etkisinin histopatolojik ve biyokimyasal incelenmesi

Öz:

Amaç: Bu çalışmada deneysel omurilik hasarı oluşturulan ratlarda shilajitin karaciğer ve böbrek üzerine olan etkisinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada omurilik hasarı oluşturulan ratlara 150 mg/kg ve 250 mg/kg dozlarda 1., 2., ve 3. günlerde shilajit tedavisi uygulanmıştır. Uygulama sonrasında ratlar 14. günde sakrifiye edilerek kan, karaciğer ve böbrek dokuları alınmıştır. Bulgular: Karaciğer ve böbrek histopatolojik olarak incelendiğinde shilajitin yüksek dozlarının dokularda daha iyi koruyucu etkiye sahip olduğu düşük doz shilajitin ise kısmi düzeyde koruyucu etki gösterdiği belirlenmiştir. Biyokimyasal analizde ise shilajit uygulanan grupların kontrol grubuna istinaden serum serum aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), laktat dehidrojenaz (LDH), kreatin kinaz (CK), kreatin ve üre konsantrasyonlarının daha düşük, albümin (ALB) ve total protein (TP) konsantrasyonlarının ise daha yüksek çıkmıştır (P aralığı: 0.05–0.001). Öneriler: Sonuç olarak, bu çalışmada ratlarda spinal kord hasarı sonrasında Shilajit uygulamasının antiinflamatuar özelliği ile karaciğer ve böbrekte lezyon oluşumunu önemli ölçüde baskıladığı histopatolojik ve biyokimyasal olarak ortaya konmuştur.

Anahtar Kelime: Böbrek karaciğer omurilik hasarı shilajit

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SANCAK T, okulmuş Ç, AKYOL M, KELES O, CETİN E (2023). Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. , 230 - 236.
Chicago	SANCAK TUNAHAN,okulmuş Çağlar,AKYOL MEHMET EDIP,KELES OMER FARUK,CETİN EYUP Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. (2023): 230 - 236.
MLA	SANCAK TUNAHAN,okulmuş Çağlar,AKYOL MEHMET EDIP,KELES OMER FARUK,CETİN EYUP Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. , 2023, ss.230 - 236.
AMA	SANCAK T,okulmuş Ç,AKYOL M,KELES O,CETİN E Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. . 2023; 230 - 236.
Vancouver	SANCAK T,okulmuş Ç,AKYOL M,KELES O,CETİN E Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. . 2023; 230 - 236.
IEEE	SANCAK T,okulmuş Ç,AKYOL M,KELES O,CETİN E "Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury." , ss.230 - 236, 2023.
ISNAD	SANCAK, TUNAHAN vd. "Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury". (2023), 230-236.

APA	SANCAK T, okulmuş Ç, AKYOL M, KELES O, CETİN E (2023). Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 37(3), 230 - 236.
Chicago	SANCAK TUNAHAN,okulmuş Çağlar,AKYOL MEHMET EDIP,KELES OMER FARUK,CETİN EYUP Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 37, no.3 (2023): 230 - 236.
MLA	SANCAK TUNAHAN,okulmuş Çağlar,AKYOL MEHMET EDIP,KELES OMER FARUK,CETİN EYUP Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, vol.37, no.3, 2023, ss.230 - 236.
AMA	SANCAK T,okulmuş Ç,AKYOL M,KELES O,CETİN E Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2023; 37(3): 230 - 236.
Vancouver	SANCAK T,okulmuş Ç,AKYOL M,KELES O,CETİN E Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2023; 37(3): 230 - 236.
IEEE	SANCAK T,okulmuş Ç,AKYOL M,KELES O,CETİN E "Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury." Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 37, ss.230 - 236, 2023.
ISNAD	SANCAK, TUNAHAN vd. "Histopathological and Biochemical Investigation of the Effect of Shilajit on Liver and Kidney in Rats with Experimental Spinal Cord Injury". Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 37/3 (2023), 230-236.