Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation

Azibe YILDIZ, (İnönü Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Malatya, Türkiye)
Muhammed Yasir ARAS, (İnönü Üniversitesi, Tıp Fakültesi, Malatya, Türkiye)
Mehmet GUNATA, (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Farmakoloji Anabilim Dalı, Malatya, Türkiye)
Merve DURHAN, (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı, Malatya, Türkiye)
Seyhan POLAT, (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Farmakoloji Anabilim Dalı, Malatya, Türkiye)
Hakan PARLAKPINAR, (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Farmakoloji Anabilim Dalı, Malatya, Türkiye)
Yilmaz CİGREMİS, (İnönü Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı, Malatya, Türkiye)
Nigar VARDİ (İnönü Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Malatya, Türkiye)
Annals of Medical Research
1 0
Yıl: 2023 Cilt: 30 Sayı: 8 Sayfa Aralığı: 899 - 904 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2023.06.128 İndeks Tarihi: 01-11-2023

Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation

Öz:
Aim: Nephrotoxicity due to the use of methotrexate (Mtx) is one of the most important problems associated with chemotherapy. Oxidative stress and inflammation are the major pathomechanisms of Mtx-induced nephrotoxicity. Alamandine (Ala), a new member of the renin-angiotensin system (RAS), is an important peptide with antioxidant and antiinflammatory capacities. In this study, it was investigated whether Ala ameliorates Mtxinduced kidney damage by reducing oxidative stress and inflammation. Materials and Methods: Male Wistar albino rats were assigned into three groups: control group, Mtx group, and Mtx+Ala group. At the end of the experiment, kidney tissues were quickly removed. Glutathione (GSH) and malondialdehyde (MDA) levels were measured to determine the oxidative state in kidney tissues. In addition, tissue samples were assessed as histopathological and immunohistochemical for heat shock protein 60 (HSP60), caspase-3, tumor necrosis factor-α (TNF-α), and receptor-interacting protein kinase-3 (RIPK3). Results: Mtx treatment resulted in reduced GSH content, elevated MDA level, increased heat shock protein 60 (HSP60), and caspase-3 expression. These changes in kidney tissues of rats treated with Mtx triggered oxidative stress characterized by apoptosis and kidney damage. Mtx also markedly increased the expression of TNF-α, an inflammation marker, and RIPK3, a marker of necroptosis. However, Ala administration significantly alleviated Mtx-induced kidney damage by reducing apoptosis and necroptosis by suppressing oxidative stress and inflammation. Conclusion: Taken together, our results support that Ala treatment can serve as a new and promising therapeutic strategy against Mtx-induced nephrotoxicity.
Anahtar Kelime: Alamandine Methotrexate Oxidative stress Inflammation Apoptosis Nephrotoxicity

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Yıldız A, Aras M, GUNATA M, Durhan M, POLAT S, Parlakpinar H, Cigremis Y, Vardi N (2023). Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. , 899 - 904. 10.5455/annalsmedres.2023.06.128
Chicago Yıldız Azibe,Aras Muhammed Yasir,GUNATA MEHMET,Durhan Merve,POLAT SEYHAN,Parlakpinar Hakan,Cigremis Yilmaz,Vardi Nigar Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. (2023): 899 - 904. 10.5455/annalsmedres.2023.06.128
MLA Yıldız Azibe,Aras Muhammed Yasir,GUNATA MEHMET,Durhan Merve,POLAT SEYHAN,Parlakpinar Hakan,Cigremis Yilmaz,Vardi Nigar Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. , 2023, ss.899 - 904. 10.5455/annalsmedres.2023.06.128
AMA Yıldız A,Aras M,GUNATA M,Durhan M,POLAT S,Parlakpinar H,Cigremis Y,Vardi N Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. . 2023; 899 - 904. 10.5455/annalsmedres.2023.06.128
Vancouver Yıldız A,Aras M,GUNATA M,Durhan M,POLAT S,Parlakpinar H,Cigremis Y,Vardi N Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. . 2023; 899 - 904. 10.5455/annalsmedres.2023.06.128
IEEE Yıldız A,Aras M,GUNATA M,Durhan M,POLAT S,Parlakpinar H,Cigremis Y,Vardi N "Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation." , ss.899 - 904, 2023. 10.5455/annalsmedres.2023.06.128
ISNAD Yıldız, Azibe vd. "Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation". (2023), 899-904. https://doi.org/10.5455/annalsmedres.2023.06.128
APA Yıldız A, Aras M, GUNATA M, Durhan M, POLAT S, Parlakpinar H, Cigremis Y, Vardi N (2023). Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. Annals of Medical Research, 30(8), 899 - 904. 10.5455/annalsmedres.2023.06.128
Chicago Yıldız Azibe,Aras Muhammed Yasir,GUNATA MEHMET,Durhan Merve,POLAT SEYHAN,Parlakpinar Hakan,Cigremis Yilmaz,Vardi Nigar Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. Annals of Medical Research 30, no.8 (2023): 899 - 904. 10.5455/annalsmedres.2023.06.128
MLA Yıldız Azibe,Aras Muhammed Yasir,GUNATA MEHMET,Durhan Merve,POLAT SEYHAN,Parlakpinar Hakan,Cigremis Yilmaz,Vardi Nigar Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. Annals of Medical Research, vol.30, no.8, 2023, ss.899 - 904. 10.5455/annalsmedres.2023.06.128
AMA Yıldız A,Aras M,GUNATA M,Durhan M,POLAT S,Parlakpinar H,Cigremis Y,Vardi N Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. Annals of Medical Research. 2023; 30(8): 899 - 904. 10.5455/annalsmedres.2023.06.128
Vancouver Yıldız A,Aras M,GUNATA M,Durhan M,POLAT S,Parlakpinar H,Cigremis Y,Vardi N Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation. Annals of Medical Research. 2023; 30(8): 899 - 904. 10.5455/annalsmedres.2023.06.128
IEEE Yıldız A,Aras M,GUNATA M,Durhan M,POLAT S,Parlakpinar H,Cigremis Y,Vardi N "Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation." Annals of Medical Research, 30, ss.899 - 904, 2023. 10.5455/annalsmedres.2023.06.128
ISNAD Yıldız, Azibe vd. "Alamandine alleviates methotrexate-induced nephrotoxicity in rats by targeting oxidative stress and inflammation". Annals of Medical Research 30/8 (2023), 899-904. https://doi.org/10.5455/annalsmedres.2023.06.128
Sayfa Oluşturulma Tarihi
24-04-2024 9:37

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