This study investigated the effects of chemical fertilizer and two different organic fertilizer (compost and wood ash solution) applications on seedling quality in tomatoes. To determine the seedling quality in the study, stem diameter (mm), seedling height (cm), number of leaves (piece), leaf chlorophyll content (CCI), leaf area (cm2), root length (cm), total seedling dry weight (g) and relative growth rate (NBH) parameters were examined. According to the results obtained, chemical fertilizer use, stem diameter (3.2mm), number of leaves (6 pieces), leaf chlorophyll content (14.7 CCI), leaf area (49.42 cm2), root length (21 cm) and total seedling dry weight (0.287g) increased their value. However, it was determined that statistically (p≤0.05) similar values were obtained in all fertilizer applications except the control. As a result of the study, it was determined that organic fertilizer applications could compete with chemical fertilizers. Wood ash increased the relative growth rate (0.110 g g-2day-1), and its effects on the seedling height (16.5 cm) were found to be statistically significant. As a result, the use of wood ash provided height control in seedlings has highlighted the use of wood ash in tomato seedling cultivation.