Yıl: 2023 Cilt: 47 Sayı: 4 Sayfa Aralığı: 290 - 300 Metin Dili: İngilizce DOI: 10.55730/1300-0152.2663 İndeks Tarihi: 20-11-2023

Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer

Öz:
Background/aim: Colorectal cancer (CRC) is a fatal malignancy type and its occurence still needs to be explored mechanistically. Notch, IL-1, and leptin crosstalk is reported to play a role in the proliferation, migration, and expression of proangiogenic molecules. In this study, we aimed to investigate the effect of inhibition of Notch, IL-1, and leptin on CRC. Materials and methods: To generate colorectal cancer tumor xenografts, 1 × 10 7 cells from exponentially growing cultures of HCT- 15 cells were injected subcutaneously, at the axillary region of the left and right rear flanks of forty $NOD.CB17-Prkd^{cscid}/J (NOD/SCID)$ female mice. The mice were then monitored for the development of tumors and were randomly divided into five groups when tumor sizes reached a volume of approximately 150 mm3. Mice were used to determine the effectiveness of the gamma-secretase inhibitor (DAPT, Notch inhibitor), the interleukin-1 receptor antagonist (Anakinra) and the leptin receptor antagonist (Allo aca) against tumor growth. The mice were euthanized by $CO_2$ inhalation immediately after the treatments finished, and all efforts were made to minimize suffering. Tumors were excissed for RT-PCR and histological analysis. Results: There is an intact Notch, IL-1, and leptin signaling axis, and in vivo antagonism of Notch, IL-1, and leptin affects mRNA and protein expression of inflammatory and angiogenic molecules. Conclusion: Present data suggest that targeting Notch, IL-1, and leptin may be possesses therapeutic potential in CRC.
Anahtar Kelime: Colorectal cancer Notch IL-1 leptin proinflammatory proangiogenic

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA OZYURT R, Erkasap N, OZKURT M, erkasap s, DIMAS K, Çakır Gündoğdu A, Ulukaya E (2023). Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. , 290 - 300. 10.55730/1300-0152.2663
Chicago OZYURT RUMEYSA,Erkasap Nilufer,OZKURT METE,erkasap serdar mustafa,DIMAS KONSTANTINOS,Çakır Gündoğdu Ayşe,Ulukaya Engin Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. (2023): 290 - 300. 10.55730/1300-0152.2663
MLA OZYURT RUMEYSA,Erkasap Nilufer,OZKURT METE,erkasap serdar mustafa,DIMAS KONSTANTINOS,Çakır Gündoğdu Ayşe,Ulukaya Engin Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. , 2023, ss.290 - 300. 10.55730/1300-0152.2663
AMA OZYURT R,Erkasap N,OZKURT M,erkasap s,DIMAS K,Çakır Gündoğdu A,Ulukaya E Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. . 2023; 290 - 300. 10.55730/1300-0152.2663
Vancouver OZYURT R,Erkasap N,OZKURT M,erkasap s,DIMAS K,Çakır Gündoğdu A,Ulukaya E Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. . 2023; 290 - 300. 10.55730/1300-0152.2663
IEEE OZYURT R,Erkasap N,OZKURT M,erkasap s,DIMAS K,Çakır Gündoğdu A,Ulukaya E "Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer." , ss.290 - 300, 2023. 10.55730/1300-0152.2663
ISNAD OZYURT, RUMEYSA vd. "Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer". (2023), 290-300. https://doi.org/10.55730/1300-0152.2663
APA OZYURT R, Erkasap N, OZKURT M, erkasap s, DIMAS K, Çakır Gündoğdu A, Ulukaya E (2023). Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. Turkish Journal of Biology, 47(4), 290 - 300. 10.55730/1300-0152.2663
Chicago OZYURT RUMEYSA,Erkasap Nilufer,OZKURT METE,erkasap serdar mustafa,DIMAS KONSTANTINOS,Çakır Gündoğdu Ayşe,Ulukaya Engin Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. Turkish Journal of Biology 47, no.4 (2023): 290 - 300. 10.55730/1300-0152.2663
MLA OZYURT RUMEYSA,Erkasap Nilufer,OZKURT METE,erkasap serdar mustafa,DIMAS KONSTANTINOS,Çakır Gündoğdu Ayşe,Ulukaya Engin Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. Turkish Journal of Biology, vol.47, no.4, 2023, ss.290 - 300. 10.55730/1300-0152.2663
AMA OZYURT R,Erkasap N,OZKURT M,erkasap s,DIMAS K,Çakır Gündoğdu A,Ulukaya E Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. Turkish Journal of Biology. 2023; 47(4): 290 - 300. 10.55730/1300-0152.2663
Vancouver OZYURT R,Erkasap N,OZKURT M,erkasap s,DIMAS K,Çakır Gündoğdu A,Ulukaya E Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer. Turkish Journal of Biology. 2023; 47(4): 290 - 300. 10.55730/1300-0152.2663
IEEE OZYURT R,Erkasap N,OZKURT M,erkasap s,DIMAS K,Çakır Gündoğdu A,Ulukaya E "Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer." Turkish Journal of Biology, 47, ss.290 - 300, 2023. 10.55730/1300-0152.2663
ISNAD OZYURT, RUMEYSA vd. "Targeting of Notch, IL-1, and leptin has therapeutic potential in xenograft colorectal cancer". Turkish Journal of Biology 47/4 (2023), 290-300. https://doi.org/10.55730/1300-0152.2663