Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification

Nurhak TATAR, (Hacettepe Üniversitesi, Nükleer Bilimler Enstitüsü, Ankara, Türkiye)
Semra AKGÖNÜLLÜ, (Hacettepe Üniversitesi, Tıp Fakültesi, Kimya Bölümü, Biyokimya Anabilim Dalı, Ankara, Türkiye)
Handan YAVUZ, (Hacettepe Üniversitesi, Tıp Fakültesi, Kimya Bölümü, Biyokimya Anabilim Dalı, Ankara, Türkiye)
Adil DENİZLİ (Hacettepe Üniversitesi, Tıp Fakültesi, Kimya Bölümü, Biyokimya Anabilim Dalı, Ankara, Türkiye)
Turkish Journal of Chemistry
5 0
Yıl: 2023 Cilt: 47 Sayı: 5 Sayfa Aralığı: 1125 - 1137 Metin Dili: İngilizce DOI: 10.55730/1300-0527.3599 İndeks Tarihi: 21-11-2023

Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification

Öz:
Dye-ligand affinity chromatography is among the increasingly popular affinity chromatography based on molecular recognition for the purification of albumin. This study focuses on the binding of Cibacron Blue F3GA ligand dye with magnetic silica particles and purification by separation. Mono-disperse silica particles with bimodal pore size distribution were employed as a high- performance adsorbent for human serum albumin (HSA) protein purification under equilibrium conditions. The synthesized ligand- dye affinity based magnetic silica particles were characterized by electron spin resonance, Fourier-transform infrared spectroscopy, scanning electron microscopy, vibrating sample magnetometer, elemental analysis, and dispersive X-ray analysis. The HSA purification performance of the proposed material in the presence of a magnetic field was relatively investigated using magnetic-based particles with similar morphologies. The maximum adsorption capacity for HSA in an artificial plasma medium was defined as 48.6 mg/g magnetic silica particle. By using the designed magnetic silica particles, 1.0 M NaCl solution was successfully utilized for obtaining quantitative desorption with HSA. However, continued HSA purification performances of magnetic-based particles were significantly lower concerning the ligand-dye magnetic silica particles. The purity of the removed albumin was about 97%. The magnetic silica particles could be utilized many times without decreasing their protein adsorption capacities remarkably.
Anahtar Kelime: Albumin purification Cibacron blue F3GA ligand dye magnetic silica particles protein purification

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Tatar N, Akgönüllü S, Yavuz Alagöz H, Denizli A (2023). Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. , 1125 - 1137. 10.55730/1300-0527.3599
Chicago Tatar Nurhak,Akgönüllü Semra,Yavuz Alagöz Handan,Denizli Adil Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. (2023): 1125 - 1137. 10.55730/1300-0527.3599
MLA Tatar Nurhak,Akgönüllü Semra,Yavuz Alagöz Handan,Denizli Adil Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. , 2023, ss.1125 - 1137. 10.55730/1300-0527.3599
AMA Tatar N,Akgönüllü S,Yavuz Alagöz H,Denizli A Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. . 2023; 1125 - 1137. 10.55730/1300-0527.3599
Vancouver Tatar N,Akgönüllü S,Yavuz Alagöz H,Denizli A Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. . 2023; 1125 - 1137. 10.55730/1300-0527.3599
IEEE Tatar N,Akgönüllü S,Yavuz Alagöz H,Denizli A "Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification." , ss.1125 - 1137, 2023. 10.55730/1300-0527.3599
ISNAD Tatar, Nurhak vd. "Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification". (2023), 1125-1137. https://doi.org/10.55730/1300-0527.3599
APA Tatar N, Akgönüllü S, Yavuz Alagöz H, Denizli A (2023). Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. Turkish Journal of Chemistry, 47(5), 1125 - 1137. 10.55730/1300-0527.3599
Chicago Tatar Nurhak,Akgönüllü Semra,Yavuz Alagöz Handan,Denizli Adil Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. Turkish Journal of Chemistry 47, no.5 (2023): 1125 - 1137. 10.55730/1300-0527.3599
MLA Tatar Nurhak,Akgönüllü Semra,Yavuz Alagöz Handan,Denizli Adil Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. Turkish Journal of Chemistry, vol.47, no.5, 2023, ss.1125 - 1137. 10.55730/1300-0527.3599
AMA Tatar N,Akgönüllü S,Yavuz Alagöz H,Denizli A Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. Turkish Journal of Chemistry. 2023; 47(5): 1125 - 1137. 10.55730/1300-0527.3599
Vancouver Tatar N,Akgönüllü S,Yavuz Alagöz H,Denizli A Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification. Turkish Journal of Chemistry. 2023; 47(5): 1125 - 1137. 10.55730/1300-0527.3599
IEEE Tatar N,Akgönüllü S,Yavuz Alagöz H,Denizli A "Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification." Turkish Journal of Chemistry, 47, ss.1125 - 1137, 2023. 10.55730/1300-0527.3599
ISNAD Tatar, Nurhak vd. "Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification". Turkish Journal of Chemistry 47/5 (2023), 1125-1137. https://doi.org/10.55730/1300-0527.3599
Sayfa Oluşturulma Tarihi
24-04-2024 9:47

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TÜBİTAK ULAKBİM TR Dizin

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