Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation

Gökhan, Aylin; Çavuşoğlu, Türker; KILIÇ, Kubilay Doğan; Şirin, Cansın; Tomruk, Canberk; Yigitturk, Gurkan; erbas, oytun; Y. Sozmen, Eser; baka, meral

doi:10.55730/1300-0144.5694

Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation

Aylin GÖKHAN, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Türker ÇAVUŞOĞLU, (İzmir Bakırçay Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Kubilay Doğan KILIÇ, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Cansın ŞİRİN, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Canberk TOMRUK, (T.C. Sağlık Bakanlığı, Samsun Eğitim ve Araştırma Hastanesi, Histoloji ve Embriyoloji Bölümü, Samsun, Türkiye)

Gürkan YİĞİTTÜRK, (Muğla Sıtkı Koçman Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Muğla, Türkiye)

Oytun ERBAŞ, (Demiroğlu Bilim Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, İstanbul, Türkiye)

Eser YILDIRIM SÖZMEN, (Ege Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, İzmir, Türkiye)

Meral BAKA (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Turkish Journal of Medical Sciences

6 1

Yıl: 2023 Cilt: 53 Sayı: 5 Sayfa Aralığı: 1281 - 1292 Metin Dili: İngilizce DOI: 10.55730/1300-0144.5694 İndeks Tarihi: 29-11-2023

Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation

Öz:

Background/aim: The subject of this study was to investigate the utility of platelet-rich plasma (PRP) in the cryopreservation process to reduce cryodamage and increase tissue viability. Materials and methods: Twenty-one female Wistar rats were randomly allocated to three groups. In Group 1 (G1), rats were not subjected to vitrification (n = 7). Group 2 (G2) was the vitrification group in which PRP was added to the basic vitrification solution (n = 7). Group 3 (G3) was the vitrification group in which fetal bovine serum was added to the basic vitrification solution (n = 7). Warmed tissues were evaluated with histochemical (HC) and immunohistochemical (IHC) staining, the TUNEL method, immunofluorescence (IF) staining, and biochemical analyses. Results: The percentages of IHC staining, TUNEL method positivity, and IF staining were significantly higher in G2 compared to both G1 and G3 (P < 0.05). G2 ovaries exhibited a significant increase in both malondialdehyde and catalase values in comparison to G1 (P < 0.05). In HC staining, degenerations in primary and secondary follicles and in ovarian tissue were more common in the PRP- supplemented group. The calcium used in PRP activation was suspected to have increased the degeneration and prevented the possible positive effects of PRP. Conclusion: To the best of our knowledge, PRP-supplemented vitrification solution was used for the first time in the literature in this study in whole rat ovarian tissue vitrification. If PRP is to be used as a component in vitrification solution for rat ovarian tissue, the use of lower amounts of calcium or different methods in PRP activation, or the use of nonactivated PRP, should be considered from the beginning.

Anahtar Kelime: Calcium cryopreservation platelet-rich plasma ovary vitrification

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Gökhan A, Çavuşoğlu T, KILIÇ K, Şirin C, Tomruk C, Yigitturk G, erbas o, Y. Sozmen E, baka m (2023). Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. , 1281 - 1292. 10.55730/1300-0144.5694
Chicago	Gökhan Aylin,Çavuşoğlu Türker,KILIÇ Kubilay Doğan,Şirin Cansın,Tomruk Canberk,Yigitturk Gurkan,erbas oytun,Y. Sozmen Eser,baka meral Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. (2023): 1281 - 1292. 10.55730/1300-0144.5694
MLA	Gökhan Aylin,Çavuşoğlu Türker,KILIÇ Kubilay Doğan,Şirin Cansın,Tomruk Canberk,Yigitturk Gurkan,erbas oytun,Y. Sozmen Eser,baka meral Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. , 2023, ss.1281 - 1292. 10.55730/1300-0144.5694
AMA	Gökhan A,Çavuşoğlu T,KILIÇ K,Şirin C,Tomruk C,Yigitturk G,erbas o,Y. Sozmen E,baka m Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. . 2023; 1281 - 1292. 10.55730/1300-0144.5694
Vancouver	Gökhan A,Çavuşoğlu T,KILIÇ K,Şirin C,Tomruk C,Yigitturk G,erbas o,Y. Sozmen E,baka m Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. . 2023; 1281 - 1292. 10.55730/1300-0144.5694
IEEE	Gökhan A,Çavuşoğlu T,KILIÇ K,Şirin C,Tomruk C,Yigitturk G,erbas o,Y. Sozmen E,baka m "Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation." , ss.1281 - 1292, 2023. 10.55730/1300-0144.5694
ISNAD	Gökhan, Aylin vd. "Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation". (2023), 1281-1292. https://doi.org/10.55730/1300-0144.5694

APA	Gökhan A, Çavuşoğlu T, KILIÇ K, Şirin C, Tomruk C, Yigitturk G, erbas o, Y. Sozmen E, baka m (2023). Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. Turkish Journal of Medical Sciences, 53(5), 1281 - 1292. 10.55730/1300-0144.5694
Chicago	Gökhan Aylin,Çavuşoğlu Türker,KILIÇ Kubilay Doğan,Şirin Cansın,Tomruk Canberk,Yigitturk Gurkan,erbas oytun,Y. Sozmen Eser,baka meral Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. Turkish Journal of Medical Sciences 53, no.5 (2023): 1281 - 1292. 10.55730/1300-0144.5694
MLA	Gökhan Aylin,Çavuşoğlu Türker,KILIÇ Kubilay Doğan,Şirin Cansın,Tomruk Canberk,Yigitturk Gurkan,erbas oytun,Y. Sozmen Eser,baka meral Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. Turkish Journal of Medical Sciences, vol.53, no.5, 2023, ss.1281 - 1292. 10.55730/1300-0144.5694
AMA	Gökhan A,Çavuşoğlu T,KILIÇ K,Şirin C,Tomruk C,Yigitturk G,erbas o,Y. Sozmen E,baka m Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. Turkish Journal of Medical Sciences. 2023; 53(5): 1281 - 1292. 10.55730/1300-0144.5694
Vancouver	Gökhan A,Çavuşoğlu T,KILIÇ K,Şirin C,Tomruk C,Yigitturk G,erbas o,Y. Sozmen E,baka m Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation. Turkish Journal of Medical Sciences. 2023; 53(5): 1281 - 1292. 10.55730/1300-0144.5694
IEEE	Gökhan A,Çavuşoğlu T,KILIÇ K,Şirin C,Tomruk C,Yigitturk G,erbas o,Y. Sozmen E,baka m "Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation." Turkish Journal of Medical Sciences, 53, ss.1281 - 1292, 2023. 10.55730/1300-0144.5694
ISNAD	Gökhan, Aylin vd. "Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation". Turkish Journal of Medical Sciences 53/5 (2023), 1281-1292. https://doi.org/10.55730/1300-0144.5694