Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells

Uslu, Burcu; yaman, mustafa; OZDEMIR SANCI, TUBA; Gungormus, Mustafa; Kopru, Cagla Zubeyde; Gunes, FEsra

doi:10.55730/1300-0144.5715

Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells

Burcu USLU, (Yüksek İhtisas Üniversitesi, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü, Ankara, Türkiye)

Mustafa YAMAN, (İstanbul Sabahattin Zaim Üniversitesi, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü, İstanbul, Türkiye)

Tuba ÖZDEMİR SANCI, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Mustafa GÜNGÖRMÜŞ, (Ankara Yıldırım Beyazıt Üniversitesi, Merkezi Araştırma Laboratuvarı Uygulama ve Araştırma Merkezi, Ankara, Türkiye)

Çağla Zübeyde KÖPRÜ, (Yüksek İhtisas Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Fatma Esra GÜNEŞ (İstanbul Medeniyet Üniversitesi, Sağlık Bilimleri Fakültesi, Beslenme ve Diyetetik Bölümü, İstanbul, Türkiye)

Turkish Journal of Medical Sciences

9 1

Yıl: 2023 Cilt: 53 Sayı: 5 Sayfa Aralığı: 1476 - 1488 Metin Dili: İngilizce DOI: 10.55730/1300-0144.5715 İndeks Tarihi: 29-11-2023

Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells

Öz:

Background/aim: This study aimed to determine the proliferation and apoptotic effects of extracts from Cornus mas L. and Berberis vulgaris fruits on human breast cancer cells (MCF-7). Materials and methods: The Cornus mas L. and Berberis vulgaris fruits, which constitute the herbal material of the study, were turned into 80% acetone extract after washing. The total phenolic content in Berberis vulgaris fruit extracts was determined calorimetrically using Folin-Ciocalteu reagent. The spectrophotometric method was used to determine the total flavonoid amount of the extracts. In order to measure the antioxidant capacity of Cornus mas L. and Berberis vulgaris fruits and extracts, DPPH Radical Scavenging Power test and Cu (II) ion reducing antioxidant capacity method were applied. Cell viability rates were determined by the XTT method. Flow cytometric measurement was performed to examine the apoptotic role of the extracts in the cell by using the Annexin-V/7-AAD commercial kit. Results: According to the data, Berberis vulgaris fruit extract appeared more effective on MCF-7 breast cancer cells in both 24 and 48 hours of exposure. Analyses made to examine the phenolic component and antioxidant capacity properties of the fruits used in the study and the results we encountered when we exposed the cell were found to be compatible with each other. Annexin-V/7-AAD method showed that the apoptotic effects of the extracts in 48 hour exposures were more effective. Conclusion: It has been determined that Cornus mas L. and Berberis vulgaris fruits, which are rich in phenolic components with high flavonoid content and high antioxidant capacities, support the apoptosis of cancer cells.

Anahtar Kelime: Apoptosis Cornus mas L. Berberis vulgaris cytotoxicity breast cancer

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Uslu B, yaman m, OZDEMIR SANCI T, Gungormus M, Kopru C, Gunes F (2023). Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. , 1476 - 1488. 10.55730/1300-0144.5715
Chicago	Uslu Burcu,yaman mustafa,OZDEMIR SANCI TUBA,Gungormus Mustafa,Kopru Cagla Zubeyde,Gunes FEsra Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. (2023): 1476 - 1488. 10.55730/1300-0144.5715
MLA	Uslu Burcu,yaman mustafa,OZDEMIR SANCI TUBA,Gungormus Mustafa,Kopru Cagla Zubeyde,Gunes FEsra Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. , 2023, ss.1476 - 1488. 10.55730/1300-0144.5715
AMA	Uslu B,yaman m,OZDEMIR SANCI T,Gungormus M,Kopru C,Gunes F Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. . 2023; 1476 - 1488. 10.55730/1300-0144.5715
Vancouver	Uslu B,yaman m,OZDEMIR SANCI T,Gungormus M,Kopru C,Gunes F Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. . 2023; 1476 - 1488. 10.55730/1300-0144.5715
IEEE	Uslu B,yaman m,OZDEMIR SANCI T,Gungormus M,Kopru C,Gunes F "Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells." , ss.1476 - 1488, 2023. 10.55730/1300-0144.5715
ISNAD	Uslu, Burcu vd. "Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells". (2023), 1476-1488. https://doi.org/10.55730/1300-0144.5715

APA	Uslu B, yaman m, OZDEMIR SANCI T, Gungormus M, Kopru C, Gunes F (2023). Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. Turkish Journal of Medical Sciences, 53(5), 1476 - 1488. 10.55730/1300-0144.5715
Chicago	Uslu Burcu,yaman mustafa,OZDEMIR SANCI TUBA,Gungormus Mustafa,Kopru Cagla Zubeyde,Gunes FEsra Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. Turkish Journal of Medical Sciences 53, no.5 (2023): 1476 - 1488. 10.55730/1300-0144.5715
MLA	Uslu Burcu,yaman mustafa,OZDEMIR SANCI TUBA,Gungormus Mustafa,Kopru Cagla Zubeyde,Gunes FEsra Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. Turkish Journal of Medical Sciences, vol.53, no.5, 2023, ss.1476 - 1488. 10.55730/1300-0144.5715
AMA	Uslu B,yaman m,OZDEMIR SANCI T,Gungormus M,Kopru C,Gunes F Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. Turkish Journal of Medical Sciences. 2023; 53(5): 1476 - 1488. 10.55730/1300-0144.5715
Vancouver	Uslu B,yaman m,OZDEMIR SANCI T,Gungormus M,Kopru C,Gunes F Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells. Turkish Journal of Medical Sciences. 2023; 53(5): 1476 - 1488. 10.55730/1300-0144.5715
IEEE	Uslu B,yaman m,OZDEMIR SANCI T,Gungormus M,Kopru C,Gunes F "Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells." Turkish Journal of Medical Sciences, 53, ss.1476 - 1488, 2023. 10.55730/1300-0144.5715
ISNAD	Uslu, Burcu vd. "Acetone extracts of Berberis vulgaris and Cornus mas L. induce apoptosis in MCF-7 breast cancer cells". Turkish Journal of Medical Sciences 53/5 (2023), 1476-1488. https://doi.org/10.55730/1300-0144.5715