Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty

Aylin BALCI ÖZYURT, (Hacettepe Üniversitesi, Eczacılık Fakültesi, Toksikoloji Anabilim Dalı, Ankara, Türkiye)
Tuba REÇBER, (Hacettepe Üniversitesi, Eczacılık Fakültesi, Analitik Kimya Anabilim Dalı, Ankara, Türkiye)
Ayşe Derya BULUŞ, (Sağlık Bakanlığı, Keçiören Eğitim ve Araştırma Hastanesi, Çocuk Endokrinoloji Bölümü, Ankara, Türkiye)
Emirhan NEMUTLU, (Hacettepe Üniversitesi, Eczacılık Fakültesi, Analitik Kimya Anabilim Dalı, Ankara, Türkiye)
Sedef KIR, (Hacettepe Üniversitesi, Eczacılık Fakültesi, Analitik Kimya Anabilim Dalı, Ankara, Türkiye)
Belma KOÇER GÜMÜŞEL, (Lokman Hekim Üniversitesi, Eczacılık Fakültesi, Toksikoloji Anabilim Dalı, Ankara, Türkiye)
Pınar ERKEKOĞLU (Hacettepe Üniversitesi, Eczacılık Fakültesi, Toksikoloji Anabilim Dalı, Ankara, Türkiye)
FABAD Journal of Pharmaceutical Sciences
1 0
Yıl: 2023 Cilt: 48 Sayı: 3 Sayfa Aralığı: 523 - 538 Metin Dili: İngilizce DOI: 10.55262/fabadeczacilik.1344851 İndeks Tarihi: 04-12-2023

Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty

Öz:
Precocious puberty (PP) is the beginning of secondary sexual characteristics before eight years of age in girls. “Central precocious puberty (CPP)” occurs due to early activation of the hypothalamuspituitary-gonad axis. “Peripheral precocious puberty (PPP)” is a rarer, and different condition that sidelines the hypothalamuspituitary-gonad (HPG) axis, and it depends on the peripheral causes. Metabolomics is the identification, and quantitation small molecule metabolites (<1000 Da) in a certain period. This study aimed to determine the plasma, and urinary metabolic profiles of girls, who were diagnosed with CPP (n=50) or PPP (n=47), and compare their results to control group (n=50). Metabolomics analysis was performed by using gas chromatography-mass spectrometry. After the complex chromatograms were deconvoluted, and aligned, the metabolites were identified using retention index libraries. The results were evaluated statistically using univariate, and multivariate analysis. Binary compressions were performed between groups, and metabolites from amino acids were found to be significantly different between the groups. These alterations in metabolites are suggested for potential biomarkers of PP; however, more comprehensive studies are needed to verify these data, and for validation. In the future, the metabolic alterations underlying different diseases, particularly those of endocrine origin, should be evaluated with mechanistic toxicological studies. This will help the researchers to develop new therapy options, particularly for PP.
Anahtar Kelime: Central precocious puberty gas chromatography-mass spectrometry metabolomics peripheral precocious puberty

Merkezi ve Periferik Erken Ergenliği Olan Kız Çocuklarında Metabolomik Çalışmalar

Öz:
Puberte prekoks (PP), kızlarda sekonder cinsel özelliklerin sekiz yaşından önce başlamasıdır. Hipotalamus hipofiz-gonad ekseninin erken aktivasyonu nedeniyle “merkezi puberte prekoks (CPP)” meydana gelir. “Periferik puberte prekoks (PPP)” hipotalamus- hipofiz-gonad (HPG) aksına bağlı olmayan daha nadir görülen farklı bir durumdur ve periferik nedenlere bağlıdır. Metabolomiks, küçük molekül metabolitlerinin (<1000 Da) belirli bir süre içinde tanımlanması ve nicelendirilmesidir. Bu çalışmada CPP (n=50) ve PPP (n=47) tanısı alan kız çocukların plazma ve idrar metabolik profillerinin belirlenmesi ve sonuçlarının kontrol grubu (n=50) ile karşılaştırılması amaçlanmıştır. Metabolomik analiz, gaz kromatografisi-kütle spektrometresi kullanılarak yapılmıştır. Karmaşık kromatogramlar ayrıştırıldıktan ve düzenlendiken sonra, metabolitler tutunma indeksi kitaplıkları kullanılarak tanımlanmıştır. Sonuçlar, tek değişkenli ve çok değişkenli analiz kullanılarak istatistiksel olarak değerlendirilmiştir. Gruplar arasında ikili karşılaştırmalar yapılmış ve amino asitlerin metabolitlerinin gruplar arasında önemli ölçüde farklı olduğu bulunmuştur. Bu metabolitlerdeki değişiklikler potansiyel PP biyobelirteçleri olarak önerilebilir; ancak bu verileri doğrulamak ve validasyon için daha kapsamlı çalışmalara ihtiyaç vardır. Gelecekte, farklı hastalıkların, özellikle endokrin kaynaklı olanların altında yatan metabolik değişikliklerin, mekanistik toksikolojik çalışmalarla değerlendirilmesi gerekmektedir. Bu, araştırmacıların özellikle PP için yeni tedavi seçenekleri geliştirmelerine yardımcı olacaktır.
Anahtar Kelime: Merkezi puberte prekoks gaz kromatografisi gaz kromatografisi-kütle spektrometrisi metabolomik periferal puberte prekoks

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Balcı Ozyurt A, Reçber T, Buluş D, Nemutlu E, Kır S, Kocer Gumusel B, ERKEKOGLU P (2023). Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. , 523 - 538. 10.55262/fabadeczacilik.1344851
Chicago Balcı Ozyurt Aylin,Reçber Tuba,Buluş Derya,Nemutlu Emirhan,Kır Sedef,Kocer Gumusel Belma,ERKEKOGLU PİNAR Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. (2023): 523 - 538. 10.55262/fabadeczacilik.1344851
MLA Balcı Ozyurt Aylin,Reçber Tuba,Buluş Derya,Nemutlu Emirhan,Kır Sedef,Kocer Gumusel Belma,ERKEKOGLU PİNAR Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. , 2023, ss.523 - 538. 10.55262/fabadeczacilik.1344851
AMA Balcı Ozyurt A,Reçber T,Buluş D,Nemutlu E,Kır S,Kocer Gumusel B,ERKEKOGLU P Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. . 2023; 523 - 538. 10.55262/fabadeczacilik.1344851
Vancouver Balcı Ozyurt A,Reçber T,Buluş D,Nemutlu E,Kır S,Kocer Gumusel B,ERKEKOGLU P Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. . 2023; 523 - 538. 10.55262/fabadeczacilik.1344851
IEEE Balcı Ozyurt A,Reçber T,Buluş D,Nemutlu E,Kır S,Kocer Gumusel B,ERKEKOGLU P "Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty." , ss.523 - 538, 2023. 10.55262/fabadeczacilik.1344851
ISNAD Balcı Ozyurt, Aylin vd. "Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty". (2023), 523-538. https://doi.org/10.55262/fabadeczacilik.1344851
APA Balcı Ozyurt A, Reçber T, Buluş D, Nemutlu E, Kır S, Kocer Gumusel B, ERKEKOGLU P (2023). Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. FABAD Journal of Pharmaceutical Sciences, 48(3), 523 - 538. 10.55262/fabadeczacilik.1344851
Chicago Balcı Ozyurt Aylin,Reçber Tuba,Buluş Derya,Nemutlu Emirhan,Kır Sedef,Kocer Gumusel Belma,ERKEKOGLU PİNAR Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. FABAD Journal of Pharmaceutical Sciences 48, no.3 (2023): 523 - 538. 10.55262/fabadeczacilik.1344851
MLA Balcı Ozyurt Aylin,Reçber Tuba,Buluş Derya,Nemutlu Emirhan,Kır Sedef,Kocer Gumusel Belma,ERKEKOGLU PİNAR Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. FABAD Journal of Pharmaceutical Sciences, vol.48, no.3, 2023, ss.523 - 538. 10.55262/fabadeczacilik.1344851
AMA Balcı Ozyurt A,Reçber T,Buluş D,Nemutlu E,Kır S,Kocer Gumusel B,ERKEKOGLU P Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. FABAD Journal of Pharmaceutical Sciences. 2023; 48(3): 523 - 538. 10.55262/fabadeczacilik.1344851
Vancouver Balcı Ozyurt A,Reçber T,Buluş D,Nemutlu E,Kır S,Kocer Gumusel B,ERKEKOGLU P Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty. FABAD Journal of Pharmaceutical Sciences. 2023; 48(3): 523 - 538. 10.55262/fabadeczacilik.1344851
IEEE Balcı Ozyurt A,Reçber T,Buluş D,Nemutlu E,Kır S,Kocer Gumusel B,ERKEKOGLU P "Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty." FABAD Journal of Pharmaceutical Sciences, 48, ss.523 - 538, 2023. 10.55262/fabadeczacilik.1344851
ISNAD Balcı Ozyurt, Aylin vd. "Metabolomic Studies in Girls With Central and Peripheral Precocious Puberty". FABAD Journal of Pharmaceutical Sciences 48/3 (2023), 523-538. https://doi.org/10.55262/fabadeczacilik.1344851
Sayfa Oluşturulma Tarihi
24-04-2024 9:51

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