Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes

Kuran, Mehmet; ŞEN, Uğur; KOÇYİĞİT, ALPER; SOYDAN, ERCAN; CEVIK, MESUT

Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes

Mesut ÇEVİK, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Dölerme ve Sun'i Tohumlama Anabilim Dalı, Samsun, Türkiye)

Uğur ŞEN, (Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi, Zootekni Bölümü, Samsun, Türkiye)

Alper KOÇYİĞİT, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Dölerme ve Sun'i Tohumlama Anabilim Dalı, Samsun, Türkiye)

Ercan SOYDAN, (Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, Samsun, Türkiye)

Mehmet KURAN (Ondokuz Mayıs Üniversitesi, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü, Samsun, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

2 0

Yıl: 2011 Cilt: 17 Sayı: 6 Sayfa Aralığı: 1009 - 1014 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes

Öz:

Çalışmanın amacı, sığır ovaryumlarından elde edilen kumulus oosit komplekslerinin (KOK) nükleer olgunlaşma ve kumulus ekspansiyonu üzerine olgunlaşma medyumuna katılan folikül stimüle edici hormon (FSH), luteinleştirici hormon (LH), epidermal büyüme faktörü (EGF) ve östradiol 17-β (E2)’nin etkisini değerlendirmektir. KOK’lar oositlerin kumulus hücreleri ile kuşatılma derecesine göre iyi ve zayıf kaliteli olarak sınıflandırılmıştır. Sonrasında KOK’lar 24 saat süreyle TCM-199 medyumunda 38.5°C ve %5 $CO _2$’li ortamda olgunlaşmaya bırakılmıştır. Medyuma eklenen hormonların kompozisyonu şu şekildedir; T1=10% (v/v) inaktif Fötal Sığır Serumu (FBS); T2=5 μg/ml bLH, 0.5 μg/ml bFSH ve 10 ng/ml EGF; T3=FBS, bLH, bFSH ve EGF; T4=1 μg/ml E2; T5=FBS ve E2. Olgunlaşma medyumuna FBS hariç FSH, LH ve EGF eklenmesinin diğer muamele grupları ile karşılaştırıldığında kumulus ekspansiyonunu artırdığı görülmüştür (P<0.05). Fakat nükleer boyama ile metafaz II (MII) safhasına ulaştığı belirlen oositlerin oranı muamele grupları arasında önemli bir fark göstermemiştir. Genel olarak tüm muamele gruplarında kumulus ekspansiyon derecesi KOK’ların kalitesiyle birlikte artış göstermiştir (P<0.05). Sonuç olarak hormon ve büyüme faktörü ilavesi kumulus ekspansiyonu ve olgunlaşma oranını stimüle etmiştir.

Anahtar Kelime: sığır gonadotropinler çekirdek östradiol sığır yetiştiriciliği oosit olgunlaşması oosit yumurtalıklar kültür ortamı inek yumurtalık follikülleri LH serum metafaz kumulus ooforus Grafiyan folikülleri in vitro kültür FSH epidermal büyüme faktörü

Konular: Veterinerlik

Sığır oositlerinin kumulus ekspansiyonu ve nükleer maturasyon üzerine serum, gonadotropinler, epidermal büyüme faktörü ve östradiol 17-Beta’nın etkileri

Öz:

The aim of the study was to evaluate the effects of follicle stimulating hormone (FSH), luteinizing hormone (LH), epidermal growth factor (EGF) and estradiol 17-β (E2) in maturation medium on nuclear maturation and cumulus expansion of cumulus-oocyte complexes (COCs) obtained from bovine ovaries. COCs were classified as good and poor quality grades based on cumulus investment. COCs were then subjected to in vitro maturation in TCM-199 in a humidified atmosphere of 5% $CO _2$ in air at 38.5°C for a period of 24 h. The combination of the hormones added to the medium was as follows: T1=10% (v/v) heat inactivated Fetal Bovine Serum (FBS); T2=5 μg/ml bLH, 0.5 μg/ml bFSH and 10 ng/ml EGF; T3=FBS, bLH, bFSH and EGF; T4=1 μg/ml E2; T5=FBS and E2. Supplementation of maturation medium with the FSH, LH, EGF irrespective to supplementation with FBS stimulated expansion of cumulus around the oocytes compared to oocytes in other treatment groups (P<0.05). However there were no significant differences between treatment groups in terms of proportion of oocytes reached to metaphase II (M II) stage determined by nuclear staining. In general cumulus expansion degree increased with the quality of COCs in all treatment groups (P<0.05). In conclusion, hormone and growth factor supplementation (FSH, LH and EGF) stimulated cumulus cell expansion and maturation rate.

Anahtar Kelime: ovarian follicles LH serum metaphase cumulus oophorus Graafian follicles in vitro culture FSH epidermal growth factor cattle gonadotropins nuclei estradiol cattle farming oocyte maturation oocytes ovaries culture media cows

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	CEVIK M, ŞEN U, KOÇYİĞİT A, SOYDAN E, Kuran M (2011). Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. , 1009 - 1014.
Chicago	CEVIK MESUT,ŞEN Uğur,KOÇYİĞİT ALPER,SOYDAN ERCAN,Kuran Mehmet Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. (2011): 1009 - 1014.
MLA	CEVIK MESUT,ŞEN Uğur,KOÇYİĞİT ALPER,SOYDAN ERCAN,Kuran Mehmet Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. , 2011, ss.1009 - 1014.
AMA	CEVIK M,ŞEN U,KOÇYİĞİT A,SOYDAN E,Kuran M Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. . 2011; 1009 - 1014.
Vancouver	CEVIK M,ŞEN U,KOÇYİĞİT A,SOYDAN E,Kuran M Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. . 2011; 1009 - 1014.
IEEE	CEVIK M,ŞEN U,KOÇYİĞİT A,SOYDAN E,Kuran M "Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes." , ss.1009 - 1014, 2011.
ISNAD	CEVIK, MESUT vd. "Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes". (2011), 1009-1014.

APA	CEVIK M, ŞEN U, KOÇYİĞİT A, SOYDAN E, Kuran M (2011). Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(6), 1009 - 1014.
Chicago	CEVIK MESUT,ŞEN Uğur,KOÇYİĞİT ALPER,SOYDAN ERCAN,Kuran Mehmet Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17, no.6 (2011): 1009 - 1014.
MLA	CEVIK MESUT,ŞEN Uğur,KOÇYİĞİT ALPER,SOYDAN ERCAN,Kuran Mehmet Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.17, no.6, 2011, ss.1009 - 1014.
AMA	CEVIK M,ŞEN U,KOÇYİĞİT A,SOYDAN E,Kuran M Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(6): 1009 - 1014.
Vancouver	CEVIK M,ŞEN U,KOÇYİĞİT A,SOYDAN E,Kuran M Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(6): 1009 - 1014.
IEEE	CEVIK M,ŞEN U,KOÇYİĞİT A,SOYDAN E,Kuran M "Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17, ss.1009 - 1014, 2011.
ISNAD	CEVIK, MESUT vd. "Effects of serum, gonadotropins, epidermal growth factor and estradiol 17-Beta on cumulus expansion and nuclear maturation of Bovine oocytes". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17/6 (2011), 1009-1014.