Yıl: 2011 Cilt: 35 Sayı: 6 Sayfa Aralığı: 689 - 695 Metin Dili: Türkçe

Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species

Öz:
Digitalis lamarckii (bodur yüksükotu) için direkt sürgün organogenezisi yolu ile etkili bir in vitro rejenerasyon protokolu ilk defa bu çalışma ile rapor edilmektedir. İki farklı deney seti kurulmuştur; ilk sette, in vitro koşullarda çimlendirilmiş fi delerden alınan yaprak eksplantları kullanılarak, 6-benzilaminopürin (BAP), kinetin, thidiazuron (TDZ) ve zeatinin farklı konsantrasyonları karşılaştırılmış, ikinci sette ise sürgün çoğaltımı amacıyla, birinci sette kültüre alınan ve çok sayıda sürgün meydana getirmiş olan eksplantlar kullanılarak, indol-3-butirik asidin (IBA), BAP, kinetin, TDZ ve zeatin ile kombinasyonları test edilmiştir. Sürgün rejenerasyonu (birinci deney seti) sonuçlarına bakıldığında, TDZ’nin, BAP’den istatistiki olarak çok daha etkili olduğu bulunmuş, 1,0 mg L-1 dozunda kullanıldığında ise eksplant başına ortalama 10,3 sürgün üreterek, en başarılı sonucu vermiştir. Sürgün çoğaltımı (ikinci deney seti) sonuçlarına bakıldığında ise, 0,2 mg L-1 IBA’nın 0,2 mg L-1 TDZ ile kombinasyonu, eksplant başına ortalama 16,5 sürgün üreterek, BAP (11,0 sürgün), zeatin (5,5 sürgün) veya kinetin (4,0 sürgün) ile kombinasyonuna göre istatistiki olarak çok daha başarılı sonuçlar vermiştir. Rejenere olan sürgünler, 0,5 mg L-1 indol-3-asetik asit (IAA) içeren ortamda kolaylıkla köklendirilmiştir. Köklendirilmiş rejenerantlar, daha sonra saksılara aktarılmış ve normal bir gelişme seyri göstererek olgunlaşma sürecini tamamlamışlardır. Bu çalışmada tanımlanan protokol ile, bitki rejenerasyonunun direkt sürgün organogenezisi yoluyla başarıldığı ortaya konulmuş, ve bunun, tıbbi önemi olan bu endemik türde büyük ölçekli kardenolit üretimi, germplasm korunması ve genetik transformasyon çalışmalarına katkı sağlaması beklenmektedir.
Anahtar Kelime: Digitalis lamarckii filiz tıbbi bitkiler bodur çeşitler bitki özütü gençleştirme yapraklar endemik bitkiler

Endemik tıbbi bir tür olan Digitalis lamarckii’nin yaprak eksplantlarından direkt sürgün rejenerasyonu

Öz:
This study reports, for the fi rst time, an effi cient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. (dwarf foxglove) via direct shoot organogenesis. Two sets of experiments were carried out; the fi rst compared diff erent concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid (IBA) with BAP, kinetin, TDZ, and zeatin for shoot multiplication from the leaf explants, which were already cultured and developed numerous shoots during the fi rst set of experiments. For shoot regeneration (the fi rst set of experiments), TDZ was the most eff ective at 1.0 mg L-1 concentration, producing a mean of 10.3 shoots per explant and was signifi cantly more eff ective than BAP. For shoot multiplication (the second set of experiments), a combination of 0.2 mg L-1 IBA with 0.2 mg L-1 TDZ produced signifi cantly more shoots per explant (16.5 shoots) than with BAP (11.0 shoots), zeatin (5.5 shoots), or kinetin (4.0 shoots). Th e regenerated shoots were readily rooted on medium containing 0.5 mg L-1 indole- 3-acetic acid (IAA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. Th e described protocol provides a simple way to regenerate plants through direct shoot organogenesis, which would be useful for a large-scale production of cardenolides, germplasm conservation, and genetic transformation studies in this medicinally important endemic species.
Anahtar Kelime: medicinal plants Digitalis lamarckii shoots plant extracts endemic plants regeneration leaves dwarf cultivars

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA VERMA S, YÜCESAN B, ŞAHİN G, GÜREL S, GÜREL E (2011). Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. , 689 - 695.
Chicago VERMA Sandeep Kumar,YÜCESAN BAHTİYAR BUHARA,ŞAHİN GÜNCE,GÜREL SONGÜL,GÜREL EKREM Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. (2011): 689 - 695.
MLA VERMA Sandeep Kumar,YÜCESAN BAHTİYAR BUHARA,ŞAHİN GÜNCE,GÜREL SONGÜL,GÜREL EKREM Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. , 2011, ss.689 - 695.
AMA VERMA S,YÜCESAN B,ŞAHİN G,GÜREL S,GÜREL E Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. . 2011; 689 - 695.
Vancouver VERMA S,YÜCESAN B,ŞAHİN G,GÜREL S,GÜREL E Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. . 2011; 689 - 695.
IEEE VERMA S,YÜCESAN B,ŞAHİN G,GÜREL S,GÜREL E "Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species." , ss.689 - 695, 2011.
ISNAD VERMA, Sandeep Kumar vd. "Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species". (2011), 689-695.
APA VERMA S, YÜCESAN B, ŞAHİN G, GÜREL S, GÜREL E (2011). Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turkish Journal of Botany, 35(6), 689 - 695.
Chicago VERMA Sandeep Kumar,YÜCESAN BAHTİYAR BUHARA,ŞAHİN GÜNCE,GÜREL SONGÜL,GÜREL EKREM Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turkish Journal of Botany 35, no.6 (2011): 689 - 695.
MLA VERMA Sandeep Kumar,YÜCESAN BAHTİYAR BUHARA,ŞAHİN GÜNCE,GÜREL SONGÜL,GÜREL EKREM Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turkish Journal of Botany, vol.35, no.6, 2011, ss.689 - 695.
AMA VERMA S,YÜCESAN B,ŞAHİN G,GÜREL S,GÜREL E Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turkish Journal of Botany. 2011; 35(6): 689 - 695.
Vancouver VERMA S,YÜCESAN B,ŞAHİN G,GÜREL S,GÜREL E Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species. Turkish Journal of Botany. 2011; 35(6): 689 - 695.
IEEE VERMA S,YÜCESAN B,ŞAHİN G,GÜREL S,GÜREL E "Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species." Turkish Journal of Botany, 35, ss.689 - 695, 2011.
ISNAD VERMA, Sandeep Kumar vd. "Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species". Turkish Journal of Botany 35/6 (2011), 689-695.