Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves
Yıl: 2023 Cilt: 23 Sayı: 2 Sayfa Aralığı: 292 - 299 Metin Dili: İngilizce DOI: 10.35414/akufemubid.1205241 İndeks Tarihi: 04-01-2024
Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves
Öz: One of the most prevalent environmental stresses that affects plants physically and physiologically is the low temperature. Since low temperature adversely affects plant productivity Researchers investigated the molecular processes that regulate the effects of cold stress on plants and discovered many cold responsive genes as well as the control mechanisms that regulate them. CBF/DREB (C repeat binding factor/dehydration response element binding factor) transcription factors induces the expression of corresponding genes in plants in response to abiotic stress conditions such as cold, drought and salinity. These transcription factors contribute to plant resistance to stress by activating genes in various pathways. Genes encoding CBF transcription factors were first discovered in Arabidopsis thaliana (L.). Later, Gene homologs were isolated and cloned from a variety of plants. They belong to the AP2/EREBP protein family. In the present study, the full-length cDNA encoding homologue of the transcription factor CBF1 was cloned from Olea europaea L. cv. Gemlik leaves. A 748 bp long cDNA encodes a hypothetical protein of 224 amino acids. BLAST analysis revealed that the CBF1 sequences of Gemlik cultivar and wild olive were nearly identical. A phylogenetic tree was built using Olea europea L. cv. Gemlik CBF1 sequence.
Anahtar Kelime: Olea europaea L. yaprağından tam uzunlukta CBF1 transkripsiyon faktörünü kodlayan gen homoloğunun klonlanması
Öz: Bitkileri fiziksel ve fizyolojik olarak etkileyen en yaygın çevresel streslerden biri düşük sıcaklıktır. Düşük sıcaklık bitki verimliliğini olumsuz etkilediğinden, araştırmacılar soğuk stresinin bitkiler üzerindeki etkilerini düzenleyen moleküler süreçleri araştırmışlar ve soğuğa duyarlı genlerle birlikte bunların kontrol mekanizmalarını da ortaya çıkarmışlardır. CBF/DREB (C tekrar bağlama faktörü/dehidrasyon yanıt elemanı bağlama faktörü) transkripsiyon faktörleri, bitkilerde soğuk, kuraklık ve tuzluluk gibi abiyotik stres koşullarına yanıt olarak ifade edilen genlerin ekspresyonunu indüklemektedir. Bu transkripsiyon faktörleri, çeşitli yolaklardaki genleri aktive ederek strese karşı bitki direncine katkıda bulunurlar. CBF transkripsiyon faktörlerini kodlayan genler ilk olarak Arabidopsis thaliana'da (L.) keşfedildi. Daha sonra çeşitli bitkilerden gen homologları klonlandı. CBF/DREB transkripsiyon faktörleri AP2/EREBP protein ailesi içinde yer alırlar. Bu çalışmada, transkripsiyon faktörü CBF1'in homologunu kodlayan tam uzunluktaki cDNA, Olea europaea L. cv. Gemlik yapraklarından klonlanmıştır. 748 bp uzunluğunda cDNA, 224 amino asitlik varsayımsal bir proteini kodlar. BLAST analizi, Gemlik çeşidinin ve yabani zeytinin CBF1 dizilerinin neredeyse aynı olduğunu ortaya çıkardı. Olea europea L. cv Gemlik CBF1 dizisi kullanılarak bir filogenetik ağaç oluşturuldu.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Agarwal PK, P Agarwal, MK Reddy, and SK Sopory, 2006. Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant Cell Reports, 25, 1263-1274.
- Baker SS, KS Wilhelm, and MF Thomashow, 1994. The 5′-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant Molecular Biology, 24, 701–713.
- Boyer JS, 1982. Plant productivity and environment. Science, 218(4571), 443–448.
- Caffagni A, N Pecchioni, E Francia, D Pagani, and J Milc, 2014. Candidate gene expression profiling in two contrasting tomato cultivars under chilling stress. Biologia Plantarum, 58, 283–295.
- Chen JQ, XP Meng, Y Zhang, M Xia, and XP Wang, 2008. Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnology Letters, 30, 2191–2198.
- Cultrera NGM, V Sarri, L Lucentini, M Ceccarelli, F Alagna, R Mariotti, S Mousavi, CG Ruiz, and L Baldoni, 2019. High levels of variation within gene sequences of Olea europaea L. Frontiers in Plant Science, 9, 1–17.
- Gilmour SJ, DG Zarka, EJ Stockinger, MP Salazar, JM Houghton, and MF Thomashow, 1998. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced CORgene expression. The Plant Journal, 16, 433–442.
- Guo HM, ZC Li, H Zhang, YZ Xin, and HM Cheng, 2011. Cloning of Cotton CBF Gene for Cold Tolerance and Its Expression in Transgenic Tobacco. Acta Agronomica Sinica, 37, 286–293.
- Huang B, LG Jin, and JY Liu, 2007. Molecular cloning and functional characterization of a DREB1/CBF-like gene (GhDREB1L) from cotton. Science in China, Series C: Life Sciences, 50, 7–14.
- Inoue H, H Nojima, and H Okayama, 1990. High efficiency transformation of Escherichia coli with plasmids. Gene, 96, 23–28.
- Jaglo-Ottosen KR, SJ Gilmour, DG Zarka, O Schabenberger, and MF Thomashow, 1998. Arabidopsis CBF1 overexpression induces COR genes and enhances freezing tolerance. Science, 280, 104–106.
- Liang N, L Yu, C Liu, Z Wang, X Zhao, and Y Zhan, 2019. Molecular cloning and expression under abiotic stresses and hormones of the ethylene response factor VII gene FmRAP2.12 from Fraxinus mandshurica. Journal of Forestry Research, 30, 1289–1300.
- Lissarre M, M Ohta, A Sato, and K Miura, 2010. Cold-responsive gene regulation during cold acclimation in plants. Plant Signaling and Behavior. Landes Bioscience, 5, 948-952.
- Liu Q, M Kasuga, Y Sakuma, H Abe, S Miura, K Yamaguchi-Shinozaki, and K Shinozaki, 1998. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell, 10, 1391–1406.
- Mizoi J, K Shinozaki, and K Yamaguchi-Shinozaki, 2012. AP2/ERF family transcription factors in plant abiotic stress responses. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1819, 86-96.
- Mosa KA, A. Ismail, and M Helmy, 2017. Introduction to Plant Stresses. Springer, 1–19.
- Palva ET and P Heino, 1997. Molecular Mechanism of Plant Cold Acclimation and Freezing Tolerance. In Plant Cold Hardiness, 3–14.
- Pino MT, A Ávila, A Molina, Z Jeknic, and THH Chen, 2013. Enhanced in vitro drought tolerance of Solanum tuberosum and Solanum commersonii plants overexpressing the ScCBF1 gene. Ciencia e Investigacion Agraria, 40, 171–184.
- Qin F, Y Sakuma, J Li, Q Liu, YQ Li, K Shinozaki, and K Yamaguchi-Shinozaki, 2004. Cloning and functional analysis of a novel DREB1/CBF transcription factor involved in cold-responsive gene expression in Zea mays L. Plant and Cell Physiology, 45, 1042–1052.
- Riechmann JL and EM Meyerowitz, 1998. The AP2/EREBP family of plant transcription factors. Biological Chemistry. Biological Chemistry, 379, :633-46.
- Shi Y, Y Ding, and S Yang, 2018. Molecular Regulation of CBF Signaling in Cold Acclimation. Trends in Plant Science, 23, 623-637.
- Stockinger EJ, SJ Gilmour, and MF Thomashow, 1997. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proceedings of the National Academy of Sciences of the United States of America, 94, 1035–1040.
- Stockinger EJ, Y Mao, MK Regier, SJ Triezenberg, and MF Thomashow, 2001. Transcriptional adaptor and histone acetyltransferase proteins in Arabidopsis and their interactions with CBF1, a transcriptional activator involved in cold-regulated gene expression. Nucleic Acids Research, 29, 1524–1533.
- Thomashow MF, 2001. So what’s new in the field of plant cold acclimation? Lots! Plant Physiology, 125, 89–93.
- Yamaguchi-Shinozaki K and K Shinozaki, 1994. A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell, 6, 251–264.
- Zarka DG, JT Vogel, D Cook, and MF Thomashow, 2003. Cold Induction of Arabidopsis CBF Genes Involves Multiple ICE (Inducer of CBF Expression) Promoter Elements and a Cold-Regulatory Circuit That Is Desensitized by Low Temperature. Plant Physiology, 133, 910–918.
- Zhang X, SG Fowler, H Cheng, Y Lou, SY Rhee, EJ Stockinger, and MF Thomashow, 2004. Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant. Arabidopsis. Plant Journal, 39, 905–919.
| APA | YURTSEVER M, Şeker M, Vural Korkut S (2023). Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. , 292 - 299. 10.35414/akufemubid.1205241 |
| Chicago | YURTSEVER Munise,Şeker Maide,Vural Korkut Senay Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. (2023): 292 - 299. 10.35414/akufemubid.1205241 |
| MLA | YURTSEVER Munise,Şeker Maide,Vural Korkut Senay Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. , 2023, ss.292 - 299. 10.35414/akufemubid.1205241 |
| AMA | YURTSEVER M,Şeker M,Vural Korkut S Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. . 2023; 292 - 299. 10.35414/akufemubid.1205241 |
| Vancouver | YURTSEVER M,Şeker M,Vural Korkut S Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. . 2023; 292 - 299. 10.35414/akufemubid.1205241 |
| IEEE | YURTSEVER M,Şeker M,Vural Korkut S "Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves." , ss.292 - 299, 2023. 10.35414/akufemubid.1205241 |
| ISNAD | YURTSEVER, Munise vd. "Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves". (2023), 292-299. https://doi.org/10.35414/akufemubid.1205241 |
| APA | YURTSEVER M, Şeker M, Vural Korkut S (2023). Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 23(2), 292 - 299. 10.35414/akufemubid.1205241 |
| Chicago | YURTSEVER Munise,Şeker Maide,Vural Korkut Senay Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi 23, no.2 (2023): 292 - 299. 10.35414/akufemubid.1205241 |
| MLA | YURTSEVER Munise,Şeker Maide,Vural Korkut Senay Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, vol.23, no.2, 2023, ss.292 - 299. 10.35414/akufemubid.1205241 |
| AMA | YURTSEVER M,Şeker M,Vural Korkut S Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi. 2023; 23(2): 292 - 299. 10.35414/akufemubid.1205241 |
| Vancouver | YURTSEVER M,Şeker M,Vural Korkut S Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi. 2023; 23(2): 292 - 299. 10.35414/akufemubid.1205241 |
| IEEE | YURTSEVER M,Şeker M,Vural Korkut S "Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves." Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 23, ss.292 - 299, 2023. 10.35414/akufemubid.1205241 |
| ISNAD | YURTSEVER, Munise vd. "Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves". Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi 23/2 (2023), 292-299. https://doi.org/10.35414/akufemubid.1205241 |
