Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water

DAS, Biswapriya; GHOSH, Sagarmoy; CHAKRABARTI, Kalyan; CHAKRABORTY, Ashis

Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water

Biswapriya DAS, (Department of Agricultural Chemistry and Soil Science Institute of Agricultural Science, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India)

Ashis CHAKRABORTY, (Department of Agronomy, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, 741252, India)

Sagarmoy GHOSH, (Department Of Microbiology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India)

Kalyan CHAKRABARTI (Department of Agricultural Chemistry and Soil Science Institute of Agricultural Science, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India)

Turkish Journal of Biology

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Yıl: 2011 Cilt: 35 Sayı: 6 Sayfa Aralığı: 671 - 678 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water

Öz:

The effect of pH of the assay media and different carbon sources in the culture media on the activities of 4 different enzymes, namely polygalacturonase (PG), pectin lyase (PNL), xylanase, and cellulase, of some pectinolytic bacteria were studied. The pectinolytic bacterial strains were isolated from the jute retting water bodies of 2 important jute growing areas of North 24 Parganas, West Bengal, India. The highest PG (12.45 to 41.0 IU/g cell wet weight) and PNL (22.55 to 75.7 U/mL) activities occurred at the alkaline pH of 8.0 and 8.5, respectively. Maximum xylanase activity (0.01 to 0.735 μmol/mL/min) occurred at pH 7.0. An acidic pH of 5.0 favored the cellulase activity (0 to 0.168 μmol/mL/ min). All the enzymes were highly substrate specific and inducible in nature. The highest PG and PNL activities were observed in the presence of pectin, whereas the highest xylanase and cellulase activities were detected in the presence of xylan and cellulose, respectively, as the sole carbon source in the media.

Anahtar Kelime: pectin lyase bacteria enzyme activity pectins polygalacturonase pH carbon sources cellulase

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DAS B, CHAKRABORTY A, GHOSH S, CHAKRABARTI K (2011). Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. , 671 - 678.
Chicago	DAS Biswapriya,CHAKRABORTY Ashis,GHOSH Sagarmoy,CHAKRABARTI Kalyan Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. (2011): 671 - 678.
MLA	DAS Biswapriya,CHAKRABORTY Ashis,GHOSH Sagarmoy,CHAKRABARTI Kalyan Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. , 2011, ss.671 - 678.
AMA	DAS B,CHAKRABORTY A,GHOSH S,CHAKRABARTI K Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. . 2011; 671 - 678.
Vancouver	DAS B,CHAKRABORTY A,GHOSH S,CHAKRABARTI K Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. . 2011; 671 - 678.
IEEE	DAS B,CHAKRABORTY A,GHOSH S,CHAKRABARTI K "Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water." , ss.671 - 678, 2011.
ISNAD	DAS, Biswapriya vd. "Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water". (2011), 671-678.

APA	DAS B, CHAKRABORTY A, GHOSH S, CHAKRABARTI K (2011). Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. Turkish Journal of Biology, 35(6), 671 - 678.
Chicago	DAS Biswapriya,CHAKRABORTY Ashis,GHOSH Sagarmoy,CHAKRABARTI Kalyan Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. Turkish Journal of Biology 35, no.6 (2011): 671 - 678.
MLA	DAS Biswapriya,CHAKRABORTY Ashis,GHOSH Sagarmoy,CHAKRABARTI Kalyan Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. Turkish Journal of Biology, vol.35, no.6, 2011, ss.671 - 678.
AMA	DAS B,CHAKRABORTY A,GHOSH S,CHAKRABARTI K Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. Turkish Journal of Biology. 2011; 35(6): 671 - 678.
Vancouver	DAS B,CHAKRABORTY A,GHOSH S,CHAKRABARTI K Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. Turkish Journal of Biology. 2011; 35(6): 671 - 678.
IEEE	DAS B,CHAKRABORTY A,GHOSH S,CHAKRABARTI K "Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water." Turkish Journal of Biology, 35, ss.671 - 678, 2011.
ISNAD	DAS, Biswapriya vd. "Studies on the effect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water". Turkish Journal of Biology 35/6 (2011), 671-678.