Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species

VERMA, Sandeep Kumar; Yücesan, Buhara; Gürel, Songül; Gürel, Ekrem

Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species

Sandeep Kumar VERMA, (Abant İzzet Baysal Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Bolu, Türkiye)

Bahtiyar Buhara YÜCESAN, (Abant İzzet Baysal Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Bolu, Türkiye)

Songül GÜREL, (Şeker Enstitüsü, Bitki Islahı Anabilim Dalı, 06790 Etimesgut, Ankara, Türkiye)

Ekrem GÜREL (Abant İzzet Baysal Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Bolu, Türkiye)

Turkish Journal of Biology

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Yıl: 2011 Cilt: 35 Sayı: 6 Sayfa Aralığı: 743 - 750 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species

Öz:

Türkiye’nin endemik tıbbi türlerinden biri olan Digitalis lamarckii Ivan. (bodur yüksükotu)’nın in vitro koşullarda çimlendirilmiş üç haftalık fidelerinden izole edilen kotiledon yaprağı segmentlerinden elde edilen kallustan, in vitro somatik embriyo ve sürgün oluşumu protokolu ilk defa bu çalışmada tanımlanmıştır. Embriyonik kallus, 0,54 μM α-naft alen asetik asit (NAA) ile 2,22 μM 6-benzilaminopürin (BAP) içeren Murashige ve Skoog (MS) ortamında elde edilmiş, ve BAP’yi (4,44 veya 8,87 μM) tek başına veya NAA (1,34, 2,69 veya 5,37 μM) ile kombinasyon halinde içeren MS ortamında kültüre alındığında, somatik embriyolar kolaylıkla gelişmişlerdir. Embriyo rejenerasyonu için en etkili hormonal kombinasyon, 1,34 μM NAA ile 8,87 μM BAP olmuş ve kotiledon yaprak eksplantı başına ortalama 37,0 embriyo elde edilmiştir. Organogenik kallus, 2,69 μM NAA ve 2,22 μM BAP içeren MS ortamında elde edilmiş, ve BAP’yi (2,22, 4,44 veya 8,87 μM) tek başına veya NAA (1,34 veya 2,69 μM) ile kombinasyon halinde içeren MS ortamında kültüre alındığında, sürgün oluşumu gözlenmiştir. En yüksek ortalama sürgün verimi (eksplant başına 5,67 sürgün), 8,87 μM BAP ve 2,69 μM NAA içeren ortamda elde edilmiştir. Sürgünler, indol-3-asetik asit (IAA) veya NAA (1,0, 2,5 veya 5,0 μM) içeren MS ortamında kolaylıkla köklendirilmiştir. Rejenere edilen bitkiler, saksılara aktarılarak, büyümeye ve olgunlaşmaya bırakılmışlardır. Rejenere edilen bitkilerin tamamına yakını, dış koşullara alıştırma sürecini, canlılıklarını koruyarak tamamlamışlardır. Tanımlanan bu protocol, D. lamarckii’nin germplasm depolanması, ticari üretim, genetik iyileştirme ve kardenolit üretimi çalışmalarına katkı sağlayabilecektir.

Anahtar Kelime: Digitalis lamarckii filiz kültür teknikleri tıbbi bitkiler IBA kotiledon gençleştirme yapraklar endemik bitkiler kallus iklime uyum somatik embryogenez organ oluşumu IAA

Konular: Biyoloji

Endemik tıbbi tür Digitalis lamarckii Ivan.’ın kotiledon yaprağı segmentlerinden indirekt somatik embriyo ve sürgün oluşumu

Öz:

This study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. The embryogenic callus was induced on Murashige and Skoog (MS) medium containing 0.54 μM α-naphthalene acetic acid (NAA) and 2.22 μM 6-benzylaminopurine (BAP). Somatic embryos developed readily when the embryogenic callus was transferred to MS medium containing BAP (4.44 or 8.87 μM) alone or BAP combined with NAA (1.34, 2.69, or 5.37 μM). The most effective hormonal combination for somatic embryogenesis was 1.34 μM NAA and 8.87 μM BAP, which produced a mean of 37.0 embryos per cotyledonary leaf explant. An organogenic callus was induced on MS medium containing 2.69 μM NAA and 2.22 μM BAP. Shoot development was observed when the organogenic callus was transferred to MS medium containing different concentrations of BAP alone (2.22, 4.44, or 8.87 μM) or BAP combined with NAA (1.34 or 2.69 μM). The highest mean number of shoots (5.67 shoots per explant) was obtained when the medium contained 8.87 μM BAP and 2.69 μM NAA. The regenerated shoots were readily rooted on MS medium containing 1.0, 2.5, or 5 μM indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. Over 90% of the regenerants survived the hardening process. The protocol described could be useful for germplasm conservation, commercial cultivation, genetic improvement, and cardenolide production studies in D. lamarckii.

Anahtar Kelime: regeneration leaves endemic plants callus acclimatization somatic embryogenesis organogenesis IAA Digitalis lamarckii shoots culture techniques medicinal plants IBA cotyledons

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	VERMA S, Yücesan B, Gürel S, Gürel E (2011). Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. , 743 - 750.
Chicago	VERMA Sandeep Kumar,Yücesan Buhara,Gürel Songül,Gürel Ekrem Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. (2011): 743 - 750.
MLA	VERMA Sandeep Kumar,Yücesan Buhara,Gürel Songül,Gürel Ekrem Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. , 2011, ss.743 - 750.
AMA	VERMA S,Yücesan B,Gürel S,Gürel E Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. . 2011; 743 - 750.
Vancouver	VERMA S,Yücesan B,Gürel S,Gürel E Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. . 2011; 743 - 750.
IEEE	VERMA S,Yücesan B,Gürel S,Gürel E "Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species." , ss.743 - 750, 2011.
ISNAD	VERMA, Sandeep Kumar vd. "Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species". (2011), 743-750.

APA	VERMA S, Yücesan B, Gürel S, Gürel E (2011). Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. Turkish Journal of Biology, 35(6), 743 - 750.
Chicago	VERMA Sandeep Kumar,Yücesan Buhara,Gürel Songül,Gürel Ekrem Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. Turkish Journal of Biology 35, no.6 (2011): 743 - 750.
MLA	VERMA Sandeep Kumar,Yücesan Buhara,Gürel Songül,Gürel Ekrem Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. Turkish Journal of Biology, vol.35, no.6, 2011, ss.743 - 750.
AMA	VERMA S,Yücesan B,Gürel S,Gürel E Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. Turkish Journal of Biology. 2011; 35(6): 743 - 750.
Vancouver	VERMA S,Yücesan B,Gürel S,Gürel E Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species. Turkish Journal of Biology. 2011; 35(6): 743 - 750.
IEEE	VERMA S,Yücesan B,Gürel S,Gürel E "Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species." Turkish Journal of Biology, 35, ss.743 - 750, 2011.
ISNAD	VERMA, Sandeep Kumar vd. "Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species". Turkish Journal of Biology 35/6 (2011), 743-750.