Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains

Karapınar, Zeynep; KARAMAN, Musa; KISADERE, İHSAN; USTA, Mustafa; Timurkan, Mehmet Ozkan

doi:10.55730/1300-0128.4324

Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains

Zeynep KARAPINAR, (Balıkesir Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Balıkesir, Türkiye)

Musa KARAMAN, (Balıkesir Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Balıkesir, Türkiye)

İhsan KISADERE, (Balıkesir Üniversitesi, Veteriner Fakültesi, Fizyoloji Anabilim Dalı, Balıkesir, Türkiye)

Mustafa USTA, (Balıkesir Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Balıkesir, Türkiye)

M. Özkan TİMURKAN (Atatürk Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Erzurum, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

2 0

Yıl: 2023 Cilt: 47 Sayı: 6 Sayfa Aralığı: 544 - 555 Metin Dili: İngilizce DOI: 10.55730/1300-0128.4324 İndeks Tarihi: 17-01-2024

Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains

Öz:

Canine parvovirus-2 (CPV-2), which is common worldwide, is a highly contagious pathogen in domestic dogs and some wild carnivore species. The agent causes a multisystemic infection in affected animals, especially acute hemorrhagic enteritis and fatal myocarditis in puppies. The study aimed to reveal the presence of virus in dogs with clinical symptoms of CPV-2 infection using virological, pathological, and immunohistochemical methods and make molecular characterization based on partial VP-2 gene of strains circulating in the region. As a result of PCR studies, 9 out of 16 rectal swab samples and 17 out of 38 blood samples were positive. A phylogenetic map was created by analyzing the partial VP2 gene sequences obtained from GenBank and the sequences obtained from this study. It was determined that 6 of the leukocyte samples obtained from the study were in CPV-2a, 5 of the leukocyte and 4 swab samples were in CPV-2b types. Amino acid differences were determined between the obtained sequences and the strains provided in GenBank. In the histopathological examination, lesions of varying degrees were found in all intestinal sections, while the most prominent lesions were seen in the ileocecal valve. Segmental or dense serosal hemorrhages were observed extending to the muscle layer and submucosa in the intestine. In the immunohistochemical examination, the most intense immune reaction was defined in the crypts. In order to control the infection, it is recommended to determine the circulating variants by conducting molecular epidemiological studies in different geographical regions, to support the research with different methods, and to develop the vaccines to be used according to the diversity of genetic variants.

Anahtar Kelime: Canine parvovirus-2 molecular characterization immunohistochemistry histopathology phylogenetic analysis

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Karapınar Z, KARAMAN M, KISADERE İ, USTA M, Timurkan M (2023). Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. , 544 - 555. 10.55730/1300-0128.4324
Chicago	Karapınar Zeynep,KARAMAN Musa,KISADERE İHSAN,USTA Mustafa,Timurkan Mehmet Ozkan Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. (2023): 544 - 555. 10.55730/1300-0128.4324
MLA	Karapınar Zeynep,KARAMAN Musa,KISADERE İHSAN,USTA Mustafa,Timurkan Mehmet Ozkan Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. , 2023, ss.544 - 555. 10.55730/1300-0128.4324
AMA	Karapınar Z,KARAMAN M,KISADERE İ,USTA M,Timurkan M Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. . 2023; 544 - 555. 10.55730/1300-0128.4324
Vancouver	Karapınar Z,KARAMAN M,KISADERE İ,USTA M,Timurkan M Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. . 2023; 544 - 555. 10.55730/1300-0128.4324
IEEE	Karapınar Z,KARAMAN M,KISADERE İ,USTA M,Timurkan M "Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains." , ss.544 - 555, 2023. 10.55730/1300-0128.4324
ISNAD	Karapınar, Zeynep vd. "Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains". (2023), 544-555. https://doi.org/10.55730/1300-0128.4324

APA	Karapınar Z, KARAMAN M, KISADERE İ, USTA M, Timurkan M (2023). Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. Turkish Journal of Veterinary and Animal Sciences, 47(6), 544 - 555. 10.55730/1300-0128.4324
Chicago	Karapınar Zeynep,KARAMAN Musa,KISADERE İHSAN,USTA Mustafa,Timurkan Mehmet Ozkan Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. Turkish Journal of Veterinary and Animal Sciences 47, no.6 (2023): 544 - 555. 10.55730/1300-0128.4324
MLA	Karapınar Zeynep,KARAMAN Musa,KISADERE İHSAN,USTA Mustafa,Timurkan Mehmet Ozkan Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. Turkish Journal of Veterinary and Animal Sciences, vol.47, no.6, 2023, ss.544 - 555. 10.55730/1300-0128.4324
AMA	Karapınar Z,KARAMAN M,KISADERE İ,USTA M,Timurkan M Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(6): 544 - 555. 10.55730/1300-0128.4324
Vancouver	Karapınar Z,KARAMAN M,KISADERE İ,USTA M,Timurkan M Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(6): 544 - 555. 10.55730/1300-0128.4324
IEEE	Karapınar Z,KARAMAN M,KISADERE İ,USTA M,Timurkan M "Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains." Turkish Journal of Veterinary and Animal Sciences, 47, ss.544 - 555, 2023. 10.55730/1300-0128.4324
ISNAD	Karapınar, Zeynep vd. "Virological and pathological investigation of Canine parvovirus-2 (CPV-2) with the assessment of the genetic variability of field strains". Turkish Journal of Veterinary and Animal Sciences 47/6 (2023), 544-555. https://doi.org/10.55730/1300-0128.4324