Yıl: 2023 Cilt: 53 Sayı: 6 Sayfa Aralığı: 1817 - 1824 Metin Dili: İngilizce DOI: 10.55730/1300-0144.5752 İndeks Tarihi: 18-01-2024

What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study

Öz:
Background/Aim: Surfactant is a surface-active substance that, in addition to its detergent effect, also has effects that reduce inflammation and fibrosis. Because of these effects, it was aimed herein to investigate the effect of intraperitoneal surfactant application on preventing postoperative peritoneal adhesion formation in a uterine horn adhesion model. Materials and methods: Twenty-one Wistar albino rats were randomly divided into 3 groups (G1–G3), as follows: G1 (n = 7): control group. The abdomen was opened and then closed; G2 (n = 7): adhesion group. The abdomen was opened. Then, a 2-cm linear incision was made over the right uterine horn, 2 mL of isotonic saline was administered intraperitoneally, and the abdomen was closed; and G3 (n = 7): treatment group. The abdomen was opened, a 2-cm linear incision was made over the right uterine horn, 2 mL (70 mg/kg) of surfactant was administered intraperitoneally, and the abdomen was closed. After 15 days, the rats were euthanized, the abdomens were reopened, and adhesion scoring was performed. After the right uterine horns were removed and fixed with 10% formalin, appropriate sections were taken from the traumatized tissue, stained with Masson’s trichrome, and fibrosis and inflammation scoring were performed. Results: The adhesion area and intensity were significantly higher in G2 than in G1 and G3 (p = 0.001) and were similar in G1 and G3 (p = 0.165). While fibrosis and inflammation were significantly higher in G2 than in G1 and G3 (p = 0.001), there was no difference between G1 and G3 (p = 0.5). Conclusion: Intraperitoneal surfactant administration at a dose of 70 mg/kg was found to be effective in preventing intraabdominal adhesion formation in a rat uterine horn model.
Anahtar Kelime: Postoperative adhesion uterine horn rat surfactant experimental model

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  • 1. Makarchian HR, Kasraianfard A, Ghaderzadeh P, Javadi SM, Ghorbanpoor M. The effectiveness of heparin, platelet- rich plasma (PRP), and silver nanoparticles on prevention of postoperative peritoneal adhesion formation in rats. Acta Cirúrgica Brasileira 2017; 32 (1): 22-27. https://doi.org/10.1590/ s0102-865020170103
  • 2. Ten Broek RPG, Stommel MWJ, Strik C, van Laarhoven CJHM, Keus F et al. Benefits and harms of adhesion barriers for abdominal surgery: a systematic review and meta-analysis. Lancet 2014; 383 (9911): 48-59. https://doi.org/10.1016/S0140- 6736(13)61687-6
  • 3. Kataria H, Singh VP. Liquid paraffin vs. hyaluronic acid in preventing intraperitoneal adhesions. The Indian Journal of Surgery 2017; 79 (6): 539-543. https://doi.org/10.1007/s12262- 016-1522-x
  • 4. Brochhausen C, Schmitt VH, Planck CN, Rajab TK, Hollemann D et al. Current strategies and future perspectives for intraperitoneal adhesion prevention. Journal of Gastrointestinal Surgery 2012; 16 (6): 1256-1274. https://doi.org/10.1007/s11605- 011-1819-9
  • 5. Sahbaz A, Aynioglu O, Isik H, Gun BD, Cengil O et al. Pycnogenol prevents peritoneal adhesions. Archives of Gynecology and Obstetrics 2015; 292 (6): 1279-1284. https://doi.org/10.1007/ s00404-015-3764-4
  • 6. Goret CC, Goret NE, Kiraz A, Ozkan OF, Karaayvaz M. The effect of pycnogenol on lymphatic nodes and adhesion in a peritoneal adhesion model in rats. Acta Cirúrgica Brasileira 2018; 33 (2): 134-143. https://doi.org/10.1590/s0102-865020180020000005
  • 7. Ryan GB, Grobéty J, Majno G. Mesothelial injury and recovery. American Journal of Pathology 1973; 71 (1): 93-112. PMCID: PMC1907217
  • 8. Kim YD, Jun YJ, Kim J, Kim CK. Effects of human adipose- derived stem cells on the regeneration of damaged visceral pleural mesothelial cells: a morphological study in a rabbit model. Interactive Cardiovascular and Thoracic Surgery 2014; 19 (3): 363-367. https://doi.org/10.1093/icvts/ivu124
  • 9. Wang N, Shao Y, Mei Y, Zhang L, Li Q et al. Novel mechanism for mesenchymal stem cells in attenuating peritoneal adhesion: accumulating in the lung and secreting tumor necrosis factor α-stimulating gene-6. Stem Cell Research and Therapy 2012; 3 (6): 51. https://doi.org/10.1186/scrt142
  • 10. Han JY, Goh RY, Seo SY, Hwang TH, Kwon HC et al. Cotransplantation of cord blood hematopoietic stem cells and culture-expanded and GM-CSF-/SCF-transfected mesenchymal stem cells in SCID mice. Journal of Korean Medical Science 2007; 22 (2): 242-247. https://doi.org/10.3346/jkms.2007.22.2.242
  • 11. Lucas PA. Stem cells for mesothelial repair: an understudied modality. International Journal of Artificial Organs 2007; 30 (6): 550-556. https://doi.org/10.1177/039139880703000613
  • 12. Kim TH, Park JS, An SS, Kang H. Inhibition of thrombin- activated fibrinolysis inhibitor decreases postoperative adhesion. Journal of Surgical Research 2015;193 (2): 560-566. https://doi. org/10.1016/j.jss.2014.07.056
  • 13. Wolcott RD, Rumbaugh KP, James G, Schultz G, Phillips P et al. Biofilm maturity studies indicate sharp debridement opens a time- dependent therapeutic window. Journal of Wound Care 2010; 19 (8): 320-328. https://doi.org/10.12968/ jowc.2010.19.8.77709
  • 14. Percival SL, Chen R, Mayer D, Salisbury AM. Mode of action of poloxamer-based surfactants in wound care and efficacy on biofilms. International Wound Journal 2018; 15 (5): 749-755. https://doi.org/10.1111/iwj.12922
  • 15. Domínguez Rivera Á, Martínez Urbina MÁ, López Y López VE. Advances on research in the use of agro-industrial waste in biosurfactant production. World Journal of Microbiology and Biotechnology 2019; 35 (10): 155. https://doi.org/10.1007/ s11274-019-2729-3
  • 16. Arslan E, Irkorucu O, Sozutek A, Cetinkunar S, Reyhan E et al. The potential efficacy of survanta (r) and seprafilm (r) on preventing intra-abdominal adhesions in rats. Acta Cirúrgica Brasileira 2016; 31 (6): 389-395. https://doi.org/10.1590/S0102- 865020160060000005
  • 17. Linsky CB, Diamond MP, Cunningham T, Constantine B, DeCherney AH et al. Adhesion reduction in the rabbit uterine horn model using an absorbable barrier, TC-7. Journal of Reproductive Medicine 1987; 32 (1): 17-20. PMID: 3560059
  • 18. Hooker GD, Taylor BM, Driman DK. Prevention of adhesion formation with use of sodium hyaluronate-based bioresorbable membrane in a rat model of ventral hernia repair with polypropylene mesh. a randomized, controlled study. Surgery 1999; 125 (2): 211-216. https://doi.org/10.1016/S0039- 6060(99)70267-9
  • 19. Schanaider A, Cotta-Pereira R, Silva PC, Macedo-Ramos H, Silva JD et al. Exogenous pulmonary surfactant prevents the development of intra-abdominal adhesions in rats. Journal of Cellular and Molecular Medicine 2016; 20 (4): 632-643. https:// doi.org/10.1111/jcmm.12758
  • 20. Yilmaz Y, Celik IH, Pampal A, Demirel G, Topal F et al. Effects of different pulmonary surfactants in the prevention of postoperative intraabdominal adhesion formation. Journal of Pediatric Surgery 2012; 47 (8): 1560-1565. https://doi. org/10.1016/j.jpedsurg.2012.01.080
  • 21. Jansen M, Treutner KH, Jansen PL, Otto J, Schmitz B et al. Phospholipids reduce the intraperitoneal adhesion of colonic tumor cells in rats and adhesion on extracellular matrix in vitro. International Journal of Colorectal Disease 2004; 19 (6): 525- 532. https://doi.org/10.1007/s00384-004-0611-7
  • 22. Aysan E, Bektas H, Ersoz F, Sarı S, Kaygusuz A et al. New and simple approach for preventing postoperative peritoneal adhesions: Do not touch the peritoneum without viscous liquid-a multivariate analysis. Obstetrics and Gynecology International 2012; 2012: 368924. https://doi.org/10.1155/2012/368924
  • 23. Urman B, Gomel V. Effect of hyaluronic acid on postoperative intraperitoneal adhesion formation and reformation in the rat model. Fertility and Sterility 1991; 56 (3): 568-570. https://doi. org/10.1016/s0015-0282(16)54559-2
  • 24. Jeong S, Schultz GS, Gibson DJ. Testing the influence of surfactant-based wound dressings on proteinase activity. International Wound Journal 2017; 14 (5): 786-790. https://doi. org/10.1111/iwj.12697
  • 25. Palumbo FP, Harding KG, Abbritti F, Bradbury S, Cech JD et al. New surfactant-based dressing product to improve wound closure rates of nonhealing wounds: a European multicenter study including 1036 patients. Wounds 2016; 28 (7): 233-240. PMID: 27428718
  • 26. Maciver AH, McCall M, James Shapiro AM. Intra-abdominal adhesions: cellular mechanisms and strategies for prevention. International Journal of Surgery 2011; 9 (8): 589-594. https:// doi.org/10.1016/j.ijsu.2011.08.008
  • 27. Stremmel W, Staffer S, Stuhrmann N, Gan-Schreier H, Gauss et al. Phospholipase A 2 of microbiota as pathogenetic determinant to induce inflammatory states in ulcerative colitis: therapeutic implications of phospholipase A2 inhibitors. Inflammatory Intestinal Diseases 2018; 2 (3): 180-187. https:// doi.org/10.1159/000486858
  • 28. Stremmel W, Hanemann A, Braun A, Stoffels S, Karner M et al. Delayed release phosphatidylcholine as new therapeutic drug for ulcerative colitis--a review of three clinical trials. Expert Opinion on Investigational Drugs 2010; 19 (12): 1623-1630. https://doi.org/10.1517/13543784.2010.535514
  • 29. Ar’Rajab A, Ahrén B, Rozga J, Bengmark S. Phosphatidylcholine prevents postoperative peritoneal adhesions: an experimental study in the rat. The Journal of Surgical Research 1991; 50 (3): 212-215. https://doi.org/10.1016/0022-4804(91)90180-t
  • 30. Snoj M, Ar’Rajab A, Ahrén B, Bengmark S. Effect of phosphatidylcholine on postoperative adhesions after small bowel anastomosis in the rat. British Journal of Surgery 1992; 79 (5): 427-429. https://doi.org/10.1002/bjs.1800790518
  • 31. Okabayashi K, Ashrafian H, Zacharakis E, Hasegawa H, Kitagawa Y et al. Adhesions after abdominal surgery: a systematic review of the incidence, distribution and severity. Japanese Journal of Surgery 2014; 44 (3): 405-420. https://doi. org/10.1007/s00595-013-0591-8
  • 32. Müller SA, Treutner KH, Tietze L, Anurov M, Titkova S et al. Efficacy of adhesion prevention and impact on wound healing of intraperitoneal phospholipids. Journal of Surgical Research 2001; 96 (1): 68-74. https://doi.org/10.1006/jsre.2000.6031
  • 33. Müller SA, Treutner KH, Tietze L, Anurov M, Titkova S et al. Influence of intraperitoneal phospholipid dosage on adhesion formation and wound healing at different intervals after surgery. Langenbeck’s Archives of Surgery 2001; 386 (4): 278- 284. https://doi.org/10.1007/s004230100212
  • 34. Müller SA, Treutner KH, Anurov M, Titkova S, Oettinger AP et al. Experimental evaluation of phospholipids and icodextrin in re-formation of peritoneal adhesions. British Journal of Surgery 2003; 90 (12): 1604-1607. https://doi.org/10.1002/bjs.4316
  • 35. Tsaousi G, Stavrou G, Fotiadis K, Kotzampassi K, Kolios G. Implementation of phospholipids as pharmacological modalities for postoperative adhesions prevention. European Journal of Pharmacology 2019; 842: 189-196. https://doi. org/10.1016/j.ejphar.2018.10.054
  • 36. Snoj M. Intra-abdominal adhesion formation is initiated by phospholipase A2. Medical Hypotheses 1993; 41 (6): 525-528. https://doi.org/10.1016/0306-9877(93)90109-4
  • 37. Chen Y, Hills BA, Hills YC. Unsaturated phosphatidylcholine and its application in surgical adhesion. Australian and New Zealand Journal of Surgery 2005; 75 (12): 1111-1114. https:// doi.org/10.1111/j.1445-2197.2005.03619.x
  • 38. Seo SH, Choi GJ, Lee OH, Kang H. Effect of methylene blue on experimental postoperative adhesion: a systematic review and meta-analysis. Public Library of Science One 2022; 17 (5): e0268178. https://doi.org/10.1371/journal.pone.0268178
  • 39. Mayes SM, Davis J, Scott J, Aguilar V, Zawko SA et al. Polysaccharide-based films for the prevention of unwanted postoperative adhesions at biological interfaces. Acta Biomaterialia 2020; 106: 92-101. https://doi.org/10.1016/j. actbio.2020.02.027
  • 40. Ruiz-Esparza GU, Wang X, Zhang X, Jimenez-Vazquez S, Diaz-Gomez L et al. Nanoengineered shear-thinning hydrogel barrier for preventing postoperative abdominal adhesions. Nano-Micro Letters 2021; 13 (1): 212. https://doi.org/10.1007/ s40820-021-00712-5
  • 41. Cai J, Guo J, Wang S. Application of polymer hydrogels in the prevention of postoperative adhesion: a review. Gels 2023; 9 (2): 98. https://doi.org/10.3390/gels9020098
APA Pala Ş, KULOĞLU T, Atilgan R, OZKAN Z, Hançer S (2023). What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. , 1817 - 1824. 10.55730/1300-0144.5752
Chicago Pala Şehmus,KULOĞLU TUNCAY,Atilgan Remzi,OZKAN ZEHRA SEMA,Hançer Serhat What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. (2023): 1817 - 1824. 10.55730/1300-0144.5752
MLA Pala Şehmus,KULOĞLU TUNCAY,Atilgan Remzi,OZKAN ZEHRA SEMA,Hançer Serhat What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. , 2023, ss.1817 - 1824. 10.55730/1300-0144.5752
AMA Pala Ş,KULOĞLU T,Atilgan R,OZKAN Z,Hançer S What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. . 2023; 1817 - 1824. 10.55730/1300-0144.5752
Vancouver Pala Ş,KULOĞLU T,Atilgan R,OZKAN Z,Hançer S What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. . 2023; 1817 - 1824. 10.55730/1300-0144.5752
IEEE Pala Ş,KULOĞLU T,Atilgan R,OZKAN Z,Hançer S "What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study." , ss.1817 - 1824, 2023. 10.55730/1300-0144.5752
ISNAD Pala, Şehmus vd. "What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study". (2023), 1817-1824. https://doi.org/10.55730/1300-0144.5752
APA Pala Ş, KULOĞLU T, Atilgan R, OZKAN Z, Hançer S (2023). What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. Turkish Journal of Medical Sciences, 53(6), 1817 - 1824. 10.55730/1300-0144.5752
Chicago Pala Şehmus,KULOĞLU TUNCAY,Atilgan Remzi,OZKAN ZEHRA SEMA,Hançer Serhat What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. Turkish Journal of Medical Sciences 53, no.6 (2023): 1817 - 1824. 10.55730/1300-0144.5752
MLA Pala Şehmus,KULOĞLU TUNCAY,Atilgan Remzi,OZKAN ZEHRA SEMA,Hançer Serhat What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. Turkish Journal of Medical Sciences, vol.53, no.6, 2023, ss.1817 - 1824. 10.55730/1300-0144.5752
AMA Pala Ş,KULOĞLU T,Atilgan R,OZKAN Z,Hançer S What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. Turkish Journal of Medical Sciences. 2023; 53(6): 1817 - 1824. 10.55730/1300-0144.5752
Vancouver Pala Ş,KULOĞLU T,Atilgan R,OZKAN Z,Hançer S What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study. Turkish Journal of Medical Sciences. 2023; 53(6): 1817 - 1824. 10.55730/1300-0144.5752
IEEE Pala Ş,KULOĞLU T,Atilgan R,OZKAN Z,Hançer S "What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study." Turkish Journal of Medical Sciences, 53, ss.1817 - 1824, 2023. 10.55730/1300-0144.5752
ISNAD Pala, Şehmus vd. "What is the impact of intraperitoneal surfactant administration against postoperative intraabdominal adhesion formation? an experimental study". Turkish Journal of Medical Sciences 53/6 (2023), 1817-1824. https://doi.org/10.55730/1300-0144.5752