Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model
Yıl: 2023 Cilt: 10 Sayı: 2 Sayfa Aralığı: 103 - 108 Metin Dili: İngilizce DOI: 10.5606/e-cvsi.2023.1481 İndeks Tarihi: 06-03-2024
Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model
Öz: Objectives: This study aims to investigate the effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. Materials and methods: A total of 16 New Zealand male rabbits weighing 2 to 3 kg were used in this study. The rabbits were divided into two groups with eight in each group as Group A and Group B. A vertical neck incision was made in an appropriate position for all group rabbits and the right carotid artery was dissected. The same artery was transected and anastomosis using 8/0 polypropylene was performed with a continuous anastomosis technique. Group A was assigned as the control group and no medication was given. Cilostazol was administrated to Group B at a dose of 25 mg/kg twice a day per oral for 21 days. At the end of Day 21, the anastomosis segments of the right carotid artery and contralateral carotid artery of all rabbits were sent to the histology laboratory for analysis. The lumen diameter, lumen area, intimal area, medial area, and intima/media area ratio were estimated. Results: In the serial sections, the mean lumen diameter of Group B was found to be significantly higher than Group A (p=0.001). The lumen area of Group B was significantly higher than Group A (p=0.001). The section series were evaluated and the area of the intima of Group B was significantly lower than Group A (p=0.001). The medial area of Group B was significantly larger than Group A (p=0.001). The intima/media area ratio was significantly higher in Group A (p=0.001). Conclusion: Cilostazol may be useful for preventing intimal hyperplasia and smooth muscle cell proliferation after vascular surgery.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Clowes A. Pathologic intimal hyperplasia as a response to vascular injury and reconstruction. In: Rutherford RB, editor. Vascular Surgery. Philadelphia: W. B. Saunders; 1995. p. 285-93.
- 2. Pauletto P, Sartore S, Pessina AC. Smooth-muscle-cell proliferation and differentiation in neointima formation and vascular restenosis. Clin Sci (Lond) 1994;87:467-79. doi: 10.1042/cs0870467.
- 3. Wang B, Zhang M, Takayama T, Shi X, Roenneburg DA, Kent KC, et al. BET bromodomain blockade mitigates intimal hyperplasia in rat carotid arteries. EBioMedicine 2015;2:1650-61. doi: 10.1016/j.ebiom.2015.09.045.
- 4. Kim JE, Sung JY, Woo CH, Kang YJ, Lee KY, Kim HS, et al. Cilostazol inhibits vascular smooth muscle cell proliferation and reactive oxygen species production through activation of AMP-activated protein kinase induced by heme oxygenase-1. Korean J Physiol Pharmacol 2011;15:203-10. doi: 10.4196/kjpp.2011.15.4.203.
- 5. Cardiovascular system - Peripheral vasodilators and related drugs - Cilostazol. British National Formulary,2008;56: Available at: http://www.bnf.org/bnf/bnf/current/119724. htm?q=%22cilostazol%22_hit [Accessed: October 27, 2022]
- 6. Center for Drug Evaluation and Research. Approval of Cilostazol. US Food and Drug Administration 1999. Available at: http://www.fda.gov /cder/news/cilostazol/ approval.htm [Accessed: October 27, 2022]
- 7. Kumar M, Bhattacharya V. Cilostazol: A new drug in the treatment intermittent claudication. Recent Pat Cardiovasc Drug Discov 2007;2:181-5. doi: 10.2174/157489007782418991.
- 8. Grouse JR 3rd, Allan MC, Elam MB. Clinical manifestation of atherosclerotic peripheral arterial disease and the role of cilostazol in treatment of intermittent claudication. J Clin Pharmacol 2002;42:1291-8. doi: 10.1177/0091270002042012002.
- 9. Hiatt WR, Money SR, Brass EP. Long-term safety of cilostazol in patients with peripheral artery disease: The CASTLE study (Cilostazol: A Study in Long-term Effects). J Vasc Surg 2008;47:330-6. doi: 10.1016/j.jvs.2007.10.009.
- 10. Curcio A, Torella D, Indolfi C. Mechanisms of smooth muscle cell proliferation and endothelial regeneration after vascular injury and stenting: Approach to therapy. Circ J 2011;75:1287-96. doi: 10.1253/circj.cj-11-0366.
- 11. Peyot ML, Gadeau AP, Dandré F, Belloc I, Dupuch F, Desgranges C. Extracellular adenosine induces apoptosis of human arterial smooth muscle cells via A(2b)-purinoceptor. Circ Res 2000;86:76-85. doi: 10.1161/01.res.86.1.76.
- 12. Dubey RK, Gillespie DG, Osaka K, Suzuki F, Jackson EK. Adenosine inhibits growth of rat aortic smooth muscle cells. Possible role of A2b receptor. Hypertension 1996;27:786-93. doi: 10.1161/01.hyp.27.3.786.
- 13. Weintraub WS. The vascular effects of cilostazol. Can J Cardiol 2006;22 Suppl B:56B-60B. doi: 10.1016/s0828- 282x(06)70987-4.
- 14. Biondi-Zoccai GG, Lotrionte M, Anselmino M, Moretti C, Agostoni P, Testa L, et al. Systematic review and meta- analysis of randomized clinical trials appraising the impact of cilostazol after percutaneous coronary intervention. Am Heart J 2008;155:1081-9. doi: 10.1016/j.ahj.2007.12.024.
- 15. Hiatt WR. Medical treatment of peripheral arterial disease and claudication. N Engl J Med 2001;344:1608-21. doi: 10.1056/NEJM200105243442108.
- 16. Inoue Y, Kimura Y, Hidaka H. Role of platelets in vascular intimal hyperplasia. Jpn J Thromb Hemost 1993;4:297.
- 17. Yamamoto K, Onoda K, Sawada Y, Fujinaga K, Imanaka- Yoshida K, Yoshida T, et al. Locally applied cilostazol suppresses neointimal hyperplasia and medial thickening in a vein graft model. Ann Thorac Cardiovasc Surg 2007;13:322-30.
- 18. Kudo FA, Kondo Y, Muto A, Miyazaki K, Dardik A, Nishibe M, et al. Cilostazol suppresses neointimal hyperplasia in canine vein grafts. Surg Today 2009;39:128-32. doi: 10.1007/ s00595-008-3819-2.
- 19. Kherallah RY, Khawaja M, Olson M, Angiolillo D, Birnbaum Y. Cilostazol: A review of basic mechanisms and clinical uses. Cardiovasc Drugs Ther 2022;36:777-92. doi: 10.1007/ s10557-021-07187-x.
| APA | KARAGÖZ U, Bilen Ç, Erdal A (2023). Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. , 103 - 108. 10.5606/e-cvsi.2023.1481 |
| Chicago | KARAGÖZ UĞUR,Bilen Çağatay,Erdal Abidin Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. (2023): 103 - 108. 10.5606/e-cvsi.2023.1481 |
| MLA | KARAGÖZ UĞUR,Bilen Çağatay,Erdal Abidin Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. , 2023, ss.103 - 108. 10.5606/e-cvsi.2023.1481 |
| AMA | KARAGÖZ U,Bilen Ç,Erdal A Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. . 2023; 103 - 108. 10.5606/e-cvsi.2023.1481 |
| Vancouver | KARAGÖZ U,Bilen Ç,Erdal A Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. . 2023; 103 - 108. 10.5606/e-cvsi.2023.1481 |
| IEEE | KARAGÖZ U,Bilen Ç,Erdal A "Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model." , ss.103 - 108, 2023. 10.5606/e-cvsi.2023.1481 |
| ISNAD | KARAGÖZ, UĞUR vd. "Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model". (2023), 103-108. https://doi.org/10.5606/e-cvsi.2023.1481 |
| APA | KARAGÖZ U, Bilen Ç, Erdal A (2023). Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. Cardiovascular surgery and interventions, 10(2), 103 - 108. 10.5606/e-cvsi.2023.1481 |
| Chicago | KARAGÖZ UĞUR,Bilen Çağatay,Erdal Abidin Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. Cardiovascular surgery and interventions 10, no.2 (2023): 103 - 108. 10.5606/e-cvsi.2023.1481 |
| MLA | KARAGÖZ UĞUR,Bilen Çağatay,Erdal Abidin Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. Cardiovascular surgery and interventions, vol.10, no.2, 2023, ss.103 - 108. 10.5606/e-cvsi.2023.1481 |
| AMA | KARAGÖZ U,Bilen Ç,Erdal A Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. Cardiovascular surgery and interventions. 2023; 10(2): 103 - 108. 10.5606/e-cvsi.2023.1481 |
| Vancouver | KARAGÖZ U,Bilen Ç,Erdal A Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model. Cardiovascular surgery and interventions. 2023; 10(2): 103 - 108. 10.5606/e-cvsi.2023.1481 |
| IEEE | KARAGÖZ U,Bilen Ç,Erdal A "Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model." Cardiovascular surgery and interventions, 10, ss.103 - 108, 2023. 10.5606/e-cvsi.2023.1481 |
| ISNAD | KARAGÖZ, UĞUR vd. "Inhibitory effect of cilostazol on intimal hyperplasia and smooth muscle cell proliferation in a rabbit carotid artery anastomosis model". Cardiovascular surgery and interventions 10/2 (2023), 103-108. https://doi.org/10.5606/e-cvsi.2023.1481 |
