Yıl: 2024 Cilt: 6 Sayı: 1 Sayfa Aralığı: 66 - 69 Metin Dili: İngilizce DOI: 10.36519/idcm.2024.295 İndeks Tarihi: 04-04-2024

Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR

Öz:
Objective: Improvements in SARS-CoV-2 diagnosis with easy, rapid and cost-effective approaches are required to control the COVID-19 pandemic. Antigen tests result in 5 to 30 minutes, providing an advantage over polymerase chain reaction (PCR) in duration. We tested the performance of the i-test COVID-19 rapid antigen test to real-time reverse transcriptase PCR in 200 symptomatic COVID-19 suspected patients. The cycle threshold (Ct) values of the patients were found to be between 21.6 and 34.4. The Ct value of 10 patients who tested positive in the PCR test was >30. We found that the sensitivity and specificity of the antigen test were 80.6 % and 93.7 %, respectively, for samples with a Ct value of <30, and overall agreement between antigen and PCR test was 91.6 % for these samples. i-test COVID-19 rapid antigen test can be used for screening in schools, factories, nursing homes, and everywhere where PCR test is unavailable.
Anahtar Kelime: COVID-19 PCR antigen

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık
  • 1 Otte Im Kampe E, Lehfeld AS, Buda S, Buchholz U, Haas W. Surveillance of COVID-19 school outbreaks, Germany, March to August 2020. Euro Surveill. 2020;25(38):2001645. [CrossRef]
  • 2 Pollock NR, Jacobs JR, Tran K, Cranston AE, Smith S, O’Kane CY, et al. Performance and implementation evaluation of the Abbott BinaxNOW rapid antigen test in a high-throughput drive-through community testing site in Massachusetts. J Clin Microbiol. 2021;59(5):e00083-21. [CrossRef]
  • 3 Antigen-detection in the diagnosis of SARS-CoV-2 infection using rapid immunoassays [Internet]. Geneva: World Health Organization (WHO). [cited September 28, 2022]. Available from: https://www.who.int/publications/i/item/antigen-detection-in-the-diagnosis-of-sars-cov-2infection-using-rapid-immunoassays
  • 4 Krüttgen A, Cornelissen CG, Dreher M, Hornef MW, Imöhl M, Kleines M. Comparison of the SARS-CoV-2 rapid antigen test to the real star SARS-CoV-2 RT PCR kit. J Virol Methods. 2021;288:114024. [CrossRef]
  • 5 Krüger LJ, Lindner AK, Gaeddert M, Tobian F, Klein J, Steinke S, et al. A multicenter clinical diagnostic accuracy study of SureStatus, an affordable, WHO emergency use-listed, rapid, point-of-care antigen-detecting diagnostic test for SARS- CoV-2. Microbiol Spectr. 2022;10(5):e0122922. [CrossRef]
  • 6 Tamura D, Yamagishi H, Morisawa Y, Mato T, Nunomiya S, Maehara Y, et al. Diagnostic accuracy of a novel SARS-CoV-2 rapid antigen test and usefulness of specimens collected from the anterior nasal cavity. Int J Infect Dis. 2022;124:199-205. [CrossRef]
  • 7 Kocagoz T, Can O, Yurttutan Uyar N, Aksoy E, Polat T, Cankaya D, et al. Simple concentration method enables the use of gargle and mouthwash instead of nasopharyngeal swab sampling for the diagnosis of COVID-19 by PCR. Eur J Clin Microbiol Infect Dis. 2021;40(12):2617-22. [CrossRef]
  • 8 Agca H, Akalin H, Saglik I, Hacimustafaoglu M, Celebi S, Ener B. Changing epidemiology of influenza and other respiratory viruses in the first year of COVID-19 pandemic. J Infect Public Health. 2021;14(9):1186-90. [CrossRef]
  • 9 Harun A, Beyza E. Viral and atypical bacterial respiratory infections in a university teaching hospital. Jpn J Infect Dis. 2019;72(5):318-22. [CrossRef]
APA YILDIZ M, AGCA H, KAYA O, SELÇUK İ, Akalın P, Akalın H (2024). Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. , 66 - 69. 10.36519/idcm.2024.295
Chicago YILDIZ Muhammet Çağrı,AGCA HARUN,KAYA Osman,SELÇUK İLKE,Akalın Pınar,Akalın Halis Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. (2024): 66 - 69. 10.36519/idcm.2024.295
MLA YILDIZ Muhammet Çağrı,AGCA HARUN,KAYA Osman,SELÇUK İLKE,Akalın Pınar,Akalın Halis Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. , 2024, ss.66 - 69. 10.36519/idcm.2024.295
AMA YILDIZ M,AGCA H,KAYA O,SELÇUK İ,Akalın P,Akalın H Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. . 2024; 66 - 69. 10.36519/idcm.2024.295
Vancouver YILDIZ M,AGCA H,KAYA O,SELÇUK İ,Akalın P,Akalın H Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. . 2024; 66 - 69. 10.36519/idcm.2024.295
IEEE YILDIZ M,AGCA H,KAYA O,SELÇUK İ,Akalın P,Akalın H "Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR." , ss.66 - 69, 2024. 10.36519/idcm.2024.295
ISNAD YILDIZ, Muhammet Çağrı vd. "Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR". (2024), 66-69. https://doi.org/10.36519/idcm.2024.295
APA YILDIZ M, AGCA H, KAYA O, SELÇUK İ, Akalın P, Akalın H (2024). Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. Infectious diseases and clinical microbiology (Online), 6(1), 66 - 69. 10.36519/idcm.2024.295
Chicago YILDIZ Muhammet Çağrı,AGCA HARUN,KAYA Osman,SELÇUK İLKE,Akalın Pınar,Akalın Halis Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. Infectious diseases and clinical microbiology (Online) 6, no.1 (2024): 66 - 69. 10.36519/idcm.2024.295
MLA YILDIZ Muhammet Çağrı,AGCA HARUN,KAYA Osman,SELÇUK İLKE,Akalın Pınar,Akalın Halis Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. Infectious diseases and clinical microbiology (Online), vol.6, no.1, 2024, ss.66 - 69. 10.36519/idcm.2024.295
AMA YILDIZ M,AGCA H,KAYA O,SELÇUK İ,Akalın P,Akalın H Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. Infectious diseases and clinical microbiology (Online). 2024; 6(1): 66 - 69. 10.36519/idcm.2024.295
Vancouver YILDIZ M,AGCA H,KAYA O,SELÇUK İ,Akalın P,Akalın H Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR. Infectious diseases and clinical microbiology (Online). 2024; 6(1): 66 - 69. 10.36519/idcm.2024.295
IEEE YILDIZ M,AGCA H,KAYA O,SELÇUK İ,Akalın P,Akalın H "Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR." Infectious diseases and clinical microbiology (Online), 6, ss.66 - 69, 2024. 10.36519/idcm.2024.295
ISNAD YILDIZ, Muhammet Çağrı vd. "Comparison of the i-test COVID-19 Rapid Antigen Test with Real-Time Reverse Transcriptase PCR". Infectious diseases and clinical microbiology (Online) 6/1 (2024), 66-69. https://doi.org/10.36519/idcm.2024.295