Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats

SAGDIC, OSMAN; ÇAKIR, Elif; ARSLANBAŞ, Ümit; SARAYMEN, BERKAY; Cetin, Aysun; canöz, özlem

Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats

Aysun ÇETİN, (Erciyes Üniversitesi, Tıp Fakültesi, Biyokimya ve Klinik Biyokimya Anabilim Dalı, Kayseri, Türkiye)

Ümit ARSLANBAŞ, (Erciyes Üniversitesi, Tıp Fakültesi, Biyokimya ve Klinik Biyokimya Anabilim Dalı, Kayseri, Türkiye)

Berkay SARAYMEN, (Erciyes Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Kayseri, Türkiye)

Özlem CANÖZ, (Erciyes Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Kayseri, Türkiye)

Ahmet ÖZTÜRK, (Erciyes Üniversitesi, Tıp Fakültesi, Biyoistatistik Anabilim Dalı, Kayseri, Türkiye)

Osman SAĞDIÇ (Erciyes Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Anabilim Dalı, Kayseri, Türkiye)

Uluslararası Hematoloji-Onkoloji Dergisi

13 6

Yıl: 2011 Cilt: 21 Sayı: 3 Sayfa Aralığı: 133 - 140 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats

Öz:

Sisplatin, kanser tedavisinde yaygın olarak kullanılan fakat tedavi dozlarında dahi karaciğer hasarı gibi istenmeyen yan etkile- re sebep olabilen bir ilaçtır. Sisplatinin karaciğer dokusunda yaptığı hasarın altında yatan mekanizma büyük oranda serbest oksijen radikallerine bağlıdır. Bu çalıﬂmada sıçanlarda sisplatin tedavisine bağlı oluﬂan karaciğer hasarında üzüm çekirdeği özütünün (ÜÇÖ) ve esansiyel kekik yağının (EKY) muhtemel koruyucu etkilerinin araﬂtırılması amaçlanmıﬂtır. Doksanaltı adet Wistar albino cinsi erkek sıçan; her biri oniki hayvandan oluﬂan sekiz gruba bölünerek (Kontrol, ÜÇÖ, EKY, ÜÇÖ+EKY, Sisp- latin, ÜÇÖ+Sisplatin, EKY+Sisplatin, ÜÇÖ+EKY+Sisplatin), on gün süreyle takip edildi. Sisplatin, kanser tedavisinde yaygın olarak kullanılan fakat tedavi dozlarında dahi karaciğer hasarı gibi istenmeyen yan etkile- re sebep olabilen bir ilaçtır. Sisplatinin karaciğer dokusunda yaptığı hasarın altında yatan mekanizma büyük oranda serbest oksijen radikallerine bağlıdır. Bu çalıﬂmada sıçanlarda sisplatin tedavisine bağlı oluﬂan karaciğer hasarında üzüm çekirdeği özütünün (ÜÇÖ) ve esansiyel kekik yağının (EKY) muhtemel koruyucu etkilerinin araﬂtırılması amaçlanmıﬂtır. Doksanaltı adet Wistar albino cinsi erkek sıçan; her biri oniki hayvandan oluﬂan sekiz gruba bölünerek (Kontrol, ÜÇÖ, EKY, ÜÇÖ+EKY, Sisp- latin, ÜÇÖ+Sisplatin, EKY+Sisplatin, ÜÇÖ+EKY+Sisplatin), on gün süreyle takip edildi.

Anahtar Kelime:

Konular: Onkoloji

Öz:

Cisplatin is a widely used anticancer drug but, it can produce undesirable effects such as hepatotoxicity even in therapeutic doses. The underlying mechanism in hepatotoxicity has been attributed to free oxygen radicals. The present study was de- signed to determine the possible protective effects of grape seed extract (GSE) and Origanum onites essential oil (OOEO) on liver toxicity induced by cisplatin. Ninetysix–male Wistar albino rats were divided into eight groups, twelve in each (Cont- rol, GSE, OOEO, GSE+OOEO, Cisplatin, GSE+ Cisplatin, OOEO+ Cisplatin, GSE+OOEO+ Cisplatin) and followed up for 10 days. Cisplatin and OOEO were injected intraperitoneally. GSE was administered with gavage. The histopathological exami- nation of liver tissues was performed by light microscope. Superoxide dismutase (SOD), glutathione peroxidase (GSH–Px) activities and malondialdehyde (MDA) levels were determined in liver tissues. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured in serum. Cisplatin-induced considerable hepatocyte damage under micros- copic examination, and an increase in MDA levels as well as a decrease in the activities of antioxidant enzymes, including SOD, GSH–Px in liver tissues and an increase in serum ALT and AST (p< 0.001). The addition of GSE and/or OOEO signi- ficantly prevented microscopic tissue damage, reversed oxidative stress parameters and biochemical values compared to the cisplatin group (p< 0.001). In conclusion GSE and OOEO may be used in adjuvant therapy to prevent cisplatin–induced hepatotoxicity, but further studies using various doses, different time intervals, and a larger number of animals need to be carried out.

Anahtar Kelime:

Konular: Onkoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Cetin A, ARSLANBAŞ Ü, SARAYMEN B, canöz ö, ÇAKIR E, SAGDIC O (2011). Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. , 133 - 140.
Chicago	Cetin Aysun,ARSLANBAŞ Ümit,SARAYMEN BERKAY,canöz özlem,ÇAKIR Elif,SAGDIC OSMAN Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. (2011): 133 - 140.
MLA	Cetin Aysun,ARSLANBAŞ Ümit,SARAYMEN BERKAY,canöz özlem,ÇAKIR Elif,SAGDIC OSMAN Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. , 2011, ss.133 - 140.
AMA	Cetin A,ARSLANBAŞ Ü,SARAYMEN B,canöz ö,ÇAKIR E,SAGDIC O Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. . 2011; 133 - 140.
Vancouver	Cetin A,ARSLANBAŞ Ü,SARAYMEN B,canöz ö,ÇAKIR E,SAGDIC O Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. . 2011; 133 - 140.
IEEE	Cetin A,ARSLANBAŞ Ü,SARAYMEN B,canöz ö,ÇAKIR E,SAGDIC O "Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats." , ss.133 - 140, 2011.
ISNAD	Cetin, Aysun vd. "Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats". (2011), 133-140.

APA	Cetin A, ARSLANBAŞ Ü, SARAYMEN B, canöz ö, ÇAKIR E, SAGDIC O (2011). Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. Uluslararası Hematoloji-Onkoloji Dergisi, 21(3), 133 - 140.
Chicago	Cetin Aysun,ARSLANBAŞ Ümit,SARAYMEN BERKAY,canöz özlem,ÇAKIR Elif,SAGDIC OSMAN Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. Uluslararası Hematoloji-Onkoloji Dergisi 21, no.3 (2011): 133 - 140.
MLA	Cetin Aysun,ARSLANBAŞ Ümit,SARAYMEN BERKAY,canöz özlem,ÇAKIR Elif,SAGDIC OSMAN Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. Uluslararası Hematoloji-Onkoloji Dergisi, vol.21, no.3, 2011, ss.133 - 140.
AMA	Cetin A,ARSLANBAŞ Ü,SARAYMEN B,canöz ö,ÇAKIR E,SAGDIC O Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. Uluslararası Hematoloji-Onkoloji Dergisi. 2011; 21(3): 133 - 140.
Vancouver	Cetin A,ARSLANBAŞ Ü,SARAYMEN B,canöz ö,ÇAKIR E,SAGDIC O Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats. Uluslararası Hematoloji-Onkoloji Dergisi. 2011; 21(3): 133 - 140.
IEEE	Cetin A,ARSLANBAŞ Ü,SARAYMEN B,canöz ö,ÇAKIR E,SAGDIC O "Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats." Uluslararası Hematoloji-Onkoloji Dergisi, 21, ss.133 - 140, 2011.
ISNAD	Cetin, Aysun vd. "Effects of grape seed extract and origanum onites essential oil on cisplatin-ınduced hepatotoxicity in rats". Uluslararası Hematoloji-Onkoloji Dergisi 21/3 (2011), 133-140.