Yıl: 2024 Cilt: 48 Sayı: 1 Sayfa Aralığı: 15 - 20 Metin Dili: İngilizce DOI: 10.4274/tpd.galenos.2024.92408 İndeks Tarihi: 11-06-2024

Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?

Öz:
Objective: The aim of this study was to evaluate the pathogenicity of Acanthamoeba strains with T4, T5, T11, and T12 genotypes by comparing the osmotolerance and thermotolerance characteristics of Acanthamoeba strains isolated from genotype groups, within species with the same genotype, and from environmental and keratitis cases. Methods: In this study, after axenic cultures of 22 Acanthamoeba strains with T4 (Neff, A, B, D, E), T5, T11, and T12 genotypes isolated from clinical and environmental samples, thermotolerance (37 °C, 39 °C and 41 °C) and osmotolerance (0.5 M, 1 M) tests were performed. Results: All strains showed growth ability at 37 °C and 0.5 M osmolarity. While all five strains isolated from patients with Acanthamoeba keratitis showed growth ability at 37 °C and 0.5 M osmolarity, no growth was detected at 41 °C and 1 M osmolarity. When the tolerance characteristics of the strains with the same genotype were evaluated, the strains with the T5 and T4E genotypes showed the same characteristics. When Acanthamoeba strains with the T4 genotype were evaluated in general, 31.25% of the strains were found to grow at 39 °C and 6.25% at 41 °C. Of the T4Neff strains, only one strain did not show the ability to reproduce at 39 °C and showed a different feature from the other strains. While the strain with the T11 genotype grew at all temperatures, the strain with the T12 genotype did not grow at 41 °C. Conclusion: According to our research results, we believe that tolerance to 39 °C and 1 M mannitol is not an indicator of pathogenicity. More studies with Acanthamoeba strains are required to clarify this issue.
Anahtar Kelime: Acanthamoeba osmotolerance thermotolerance genotype pathogen

Acanthamoeba Türlerinin Termotolerant ve Osmotolerant Özellikleri Patojenite Göstergesi midir?

Öz:
Amaç: T4, T5, T11 ve T12 genotipine sahip Acanthamoeba suşlarında genotip grupları arasında, aynı genotipe sahip türler içerisinde ve çevresel ve keratit olgularından izole edilen Acanthamoeba suşlarının osmotolerans ve termotolerans özelliklerini karşılaştırarak patojenlikle ilişkisini değerlendirmektir. Yöntemler: Bu çalışmada, klinik ve çevresel örneklerden izole edilmiş T4 (Neff, A, B, D, E), T5, T11 ve T12 genotipine sahip sahip 22 tane Acanthamoeba suşunun aksenik kültürleri yapıldıktan sonra termotolerans (37 °C, 39 °C ve 41 °C) ve osmotolerans (0,5 M, 1 M) testleri yapılmıştır. Bulgular: Bütün suşlar 37 °C ve 0,5 M osmolaritede üreme yeteneği göstermiştir. Acanthamoeba keratitli hastalardan izole edilen beş suşun tamamı 37 °C’de ve 0,5 M osmolaritede üreme yeteneği gösterirken, 41 °C ve 1 M osmolaritede üreme saptanmamıştır. Aynı genotipe sahip suşların tolerans özellikleri değerlendirildiğinde T5 ve T4E genotipine sahip suşlar aynı özellikte bir durum sergilemiştir. T4 genotipine sahip Acanthamoeba suşları genel olarak değerlendirildiğinde 39 °C’de suşların %31,25, 41 °C’de %6,25 suşta üreme saptandı. T4Neff suşlarından sadece bir suş 39 °C’de üreme yeteneği göstermeyerek gruptan ayrı bir özellik göstermiştir. T11 genotipine suş bütün ısı derecelerinde ürerken, T12 genotipine sahip 41 °C’de ürememiştir. Sonuç: Araştırma sonuçlarımıza göre özellikle 39 °C ve 1 M mannitol toleransın patojenlik göstergesi olmadığı kanısındayız. Bu konunun açıklığa kavuşabilmesi için daha çok Acanthamoeba suşu ile çalışmaya ihtiyaç vardır.
Anahtar Kelime: Acanthamoeba osmotolerans termotolerans genotip patojen

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KAHRAMAN M, Akın Polat Z (2024). Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. , 15 - 20. 10.4274/tpd.galenos.2024.92408
Chicago KAHRAMAN Merve,Akın Polat Zübeyda Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. (2024): 15 - 20. 10.4274/tpd.galenos.2024.92408
MLA KAHRAMAN Merve,Akın Polat Zübeyda Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. , 2024, ss.15 - 20. 10.4274/tpd.galenos.2024.92408
AMA KAHRAMAN M,Akın Polat Z Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. . 2024; 15 - 20. 10.4274/tpd.galenos.2024.92408
Vancouver KAHRAMAN M,Akın Polat Z Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. . 2024; 15 - 20. 10.4274/tpd.galenos.2024.92408
IEEE KAHRAMAN M,Akın Polat Z "Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?." , ss.15 - 20, 2024. 10.4274/tpd.galenos.2024.92408
ISNAD KAHRAMAN, Merve - Akın Polat, Zübeyda. "Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?". (2024), 15-20. https://doi.org/10.4274/tpd.galenos.2024.92408
APA KAHRAMAN M, Akın Polat Z (2024). Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. Türkiye Parazitoloji Dergisi, 48(1), 15 - 20. 10.4274/tpd.galenos.2024.92408
Chicago KAHRAMAN Merve,Akın Polat Zübeyda Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. Türkiye Parazitoloji Dergisi 48, no.1 (2024): 15 - 20. 10.4274/tpd.galenos.2024.92408
MLA KAHRAMAN Merve,Akın Polat Zübeyda Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. Türkiye Parazitoloji Dergisi, vol.48, no.1, 2024, ss.15 - 20. 10.4274/tpd.galenos.2024.92408
AMA KAHRAMAN M,Akın Polat Z Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. Türkiye Parazitoloji Dergisi. 2024; 48(1): 15 - 20. 10.4274/tpd.galenos.2024.92408
Vancouver KAHRAMAN M,Akın Polat Z Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?. Türkiye Parazitoloji Dergisi. 2024; 48(1): 15 - 20. 10.4274/tpd.galenos.2024.92408
IEEE KAHRAMAN M,Akın Polat Z "Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?." Türkiye Parazitoloji Dergisi, 48, ss.15 - 20, 2024. 10.4274/tpd.galenos.2024.92408
ISNAD KAHRAMAN, Merve - Akın Polat, Zübeyda. "Are Thermotolerant and Osmotolerant Characteristics of Acanthamoeba Species an Indicator of Pathogenicity?". Türkiye Parazitoloji Dergisi 48/1 (2024), 15-20. https://doi.org/10.4274/tpd.galenos.2024.92408