Background and design: The cause of rosacea is unknown. Various etiologic factors, vascular dysfunction, genetic predisposition, gastrointestinal intolerance, Demodex folliculorum infestation, immunologic and psychological factors may be involved in the pathogenesis of rosacea. In our prospective study, the presence of specific antibodies against food and house dust mite had been investigated in the rosacea patients. Materials and Methods: Seventy-three patients with rosacea and 65 control cases were enrolled into the study. Total IgE and specific IgE antibodies directed to some food and house dust mite antigens were detected by chemiluminescent enzyme immunometric assay and immunoenzymometric assay in both groups. Results were evaluated statistically by means of Student's t test, chi -square test, Yate's corrected chi-square test and Fisher's exact chi-square test Results: Specific IgE antibodies against egg yolk, gluten, rye, white bean, chocolate, almond, strawberry, peach, banana and Dermatophagoides pteronyssinus, Storage mite lepdoglyphis des were found significantly higher than controls. We conclude that various foods and house dust mites may play a role by means of humoral immunity in the pathogenesis of rosacea. But we think that these results have to be confirmed by the studies including more patients.