Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

ADELOWO, Olawale Olufemi; FAGADE, Obasola Ezekiel

Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

Olawale Olufemi ADELOWO, (Department, of Pure and Applied Biology Ladoke Akintola Univercity of Technology PMB 4000 Ogbomoso, Nigeria)

Obasola Ezekiel FAGADE (Environmental Microbiology and Biotechnology Laboratory, Department of Botany and Microbiology, University of Ibadan, Ibadan, Nigeria)

Turkish Journal of Biology

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Yıl: 2012 Cilt: 36 Sayı: 1 Sayfa Aralığı: 37 - 45 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria

Öz:

Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. Th e present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 μg/mL of tetracycline, their identities were determined by PCR amplifi cation, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. Th e molecular basis of resistance among the isolates was also investigated by the PCR amplifi cation of class 1 and 2 integrons, tetA, tetB, tetC, tetD, tetE, tetG, tetL, tetW, tetM, tet37, $bla _{TEM},$ aadA1, strA, strB, sul2, sul3 and a 550bp gene fragment encoding a multidrug resistance efflux transporter of the resistance nodulation division (RND) family. All the isolates were resistant to the tested antimicrobials with MIC ranging from <16 μg/mL to 512 μg/mL. class 2 integrons tetB, tetC, tetD, tetE, tetG, tetL, tetW, tetM, tet37, aadA1, and sul3 could not be detected in any of the isolates. However, 2 isolates were positive for class 1 integrons, 1 each for blaTEM and tetA, 3 for sul2, and 2 for strA and strB. Seven of the isolates were positive for the RND efflux pump. Results of the present study showed that polluted aquatic ecosystems may serve as a reservoir of transferable antibiotic resistance.

Anahtar Kelime: antibiotics bacteria molecular genetics ribosomal RNA water pollution isolation rivers livestock farming antibiotic sensivity antibiotic residues identification Nigeria

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ADELOWO O, FAGADE O (2012). Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. , 37 - 45.
Chicago	ADELOWO Olawale Olufemi,FAGADE Obasola Ezekiel Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. (2012): 37 - 45.
MLA	ADELOWO Olawale Olufemi,FAGADE Obasola Ezekiel Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. , 2012, ss.37 - 45.
AMA	ADELOWO O,FAGADE O Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. . 2012; 37 - 45.
Vancouver	ADELOWO O,FAGADE O Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. . 2012; 37 - 45.
IEEE	ADELOWO O,FAGADE O "Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria." , ss.37 - 45, 2012.
ISNAD	ADELOWO, Olawale Olufemi - FAGADE, Obasola Ezekiel. "Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria". (2012), 37-45.

APA	ADELOWO O, FAGADE O (2012). Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. Turkish Journal of Biology, 36(1), 37 - 45.
Chicago	ADELOWO Olawale Olufemi,FAGADE Obasola Ezekiel Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. Turkish Journal of Biology 36, no.1 (2012): 37 - 45.
MLA	ADELOWO Olawale Olufemi,FAGADE Obasola Ezekiel Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. Turkish Journal of Biology, vol.36, no.1, 2012, ss.37 - 45.
AMA	ADELOWO O,FAGADE O Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. Turkish Journal of Biology. 2012; 36(1): 37 - 45.
Vancouver	ADELOWO O,FAGADE O Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria. Turkish Journal of Biology. 2012; 36(1): 37 - 45.
IEEE	ADELOWO O,FAGADE O "Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria." Turkish Journal of Biology, 36, ss.37 - 45, 2012.
ISNAD	ADELOWO, Olawale Olufemi - FAGADE, Obasola Ezekiel. "Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria". Turkish Journal of Biology 36/1 (2012), 37-45.