The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients

AŞGIN, NERGİS; TEVFİK, Mehmet Refik; AĞEL, Esra; DURMAZ, Bengül

The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients

Esra AĞEL, (İnönü Üniversitesi, Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Malatya, Türkiye)

Bengül DURMAZ, (Girilmedi)

Mehmet Refik TEVFİK, (Girilmedi)

Nergis AŞGIN (Girilmedi)

Turkish Journal of Medical Sciences

1 0

Yıl: 2000 Cilt: 30 Sayı: 2 Sayfa Aralığı: 143 - 146 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients

Öz:

In order to determine the isolation rates of Helicobacter pylori in dyspeptic patients, 167 biopsy specimens were examined using different methods, including culturing, microscopy and the rapid urease test. Resistance to commonly used antimicrobials was investigated using the disk diffusion method. Of the 167 specimens, 82(49.1%) were found to be positive for Helicobacter pylori in culture and 72(43 %) were positive in the urease biopsy test. Using Gram staining, Helicobacter pylori were observed in 43 (25.7 %) of the specimens. The resistance rate of the isolates was 53 % for metronidazol, 8% for both clarithromycin and ciprofloxacin, and 4% for tetracyline. The rate of resistance for amoxycillin was not recorded. Our results indicate that a susceptibility test is necessary before initiating therapy for Helicobacter pyloriinfections, otherwise treatment may be ineffective.

Anahtar Kelime: Dyspepsia Helicobacter pylori Drug Resistance, Microbial Bacterial Typing Techniques Biopsy Antibiotics Culture Media Microbial Sensitivity Tests Antibiotic susceptibility Helicobacter Infections Antibiotic resistance Isolation Bacteriological Techniques Bacterial Infections Gastric Mucosa

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

1. Blaster, M. J. Helicobacter pylori: Its role in disease. Clin Infect Dis 15: 386- 393, 1992.
2. Tayler, D. N and Blaser M. J. The epidemiology of Helicobacter pylori infection. Epidemol Rev 13: 42 –59, 1991.
3. Graham DY. Evolution of concepts regarding H. pylori: from a cause of gastritis to a public health problem (Editorial). Am J Gastroenterol 89: 469-472, 1994.
4. Bardhan PK. Epidemiological features of H. pylori infection in developing countries. Clin Infect Dis 25: 973-8, 1997.
5. Ito Y, Azuma T, Ito S, Miyaji H, Hirai M, Yamazaki Y, Sato F, Kato T, Kohli Y, Kuriyama M. Analysis and typing of the vag A gene from cag A positive strains of H. pylori isolated in Japan. J Clin Microbiol 35: 1710-1714, 1997.
6. Lage, AP, Godfroid E, Faunconnier A, Burette A, Butzler JP, Bollen A, Glupezynsky Y. Diagnosis of Helicobacter pylori infection by PCR: comparison with other invasive techniques and detection of cag-A gene in gastric biopsy specimens. Clin. Microb 33: 2752-2756, 1995.
7. Graham DY, Garacia PJM. Epidemiology and transmission. 9th International Workshop on Gastroduodenal Pathology and H. pylori. Budapest, Hungary, 1998 (http: // www. Helicobacter. Org/ehpsgworhshop- buda-work 9. htm ).
8. H pylori: When to treat (Editorial). Ann Int Med 126: 315-316, 1997.
9. Jerris R.C. Helicobacter. Manual of Clinical Microbiology (Eds. P. Murray, E. Baron, M. Ptaller, F. Tenover and R.Yolken). ASM Press. Washington DC, 1995. pp: 492-498
10. Piccolomini R, Bonaventura G, Neri M, Girollamo A, Catamo G, Pizzigallo E. Usefulness of Leifson staining method in diagnosis of H. pylori infection. J Clin Microbiol 37: 199-201, 1999.
11. Megraud F. Advantages and disadvantages of current diagnostic tests for the detection of H. pylori. Scand J Gastroenterol 215: 57-62, 1996.
12. Piccolomini R, Bonaventura G, Catamo G, Carbone F, Neri M. Optimal combination of media for primary isolation of H. pylori from gastric biopsy specimens. J Clinical Microbiol 35: 1541-1544, 1997.
13. Elmer WK. Color Atlas of Diagnostic Microbiology. Lippincott. Philadelphia, New York, 1997, pp : 337-338.
14. Glupczyski Y. Culture of H. pylori from gastric biopsies and antimicrobial susceptibility testing Helicobacter pylori techniques for clinical diagnosis and basic research (Eds. A. Lee and F. Megraud) Saunders Comp. London, 1996, pp : 16-27.
15. Henriksen TH, Lia, A. A simple method for determining metronidazole resistance of H. pylori. Clin Microb 35: 1424-1426, 1997.
16. Rosendal R, Kuipers EJ, Pena AS. Recovery of H. pylori from gastric biopsy specimens is not dependent on the transport medium used. J Cin Microbiol 33: 2798-2800,1995.
17. Glupczyski Y. Diagnosis of H. pylori infection. 9th International Workshop on Gastroduodenal Pathology and H. pylori. Budapest, Hungary, 2-5 September 1998 (http: // www. Helicobacter. Org/ehpsg- worhshopbuda- work 9. htm ). 18. Granstrom M, Tindberg Y, Blennow M. Seroepidemiology of H. pylori infection in a cohort of children monitored from 6 months to 11 years of age. J Clin Microbiol 35: 468-470, 1997.
19. Taviloglu K, Türkoglu S, Küçüker M, Akyüz A, Bügra D. Büyükuncu Y. Ang Ö. H. pylori tanısında serolojik yöntemin histolojik ve mikrobiyolojik yöntemler ile karsılastırılması. T Mikrobiyol Cem Derg 24: 179-183, 1994.
20. Büke AÇ, Aydın A, Tünger A, Günar F, Ersöz, G,Saydam C, Günhan C. H. pylori’nin amoksisilin, klaritromisin ve metronidazol’e karsı duyarlılıgının disk diffüzyon yöntemi ile arastırılması. Tr J Infect 11: 267-269, 1997.
21. Kalkan A, Bulut V, Bahçecioglu AH, Demirdag K, Aral M. Elazıg yöresinde H. pylori infeksiyon seroprevalansı. Türk Mikrobiol Cem Derg 27:28- 32,1997
22. Aydın F, Köseahmet F, Katırcıoglu, Albayrak L, Çalık A, Bingöl R. Antral mide biyopsi örnekerinde H. pylori’nin belirlenmesinde kullanılan bazı yöntemlerin duyarlılık ve özgüllükleri. Türk Mikrobiyol Cem Derg 24:176- 178, 1994
23. Aladag A, Akgün Y, Sahintürk V, Gürer F, Sarıçam T: H. pylori saptanmasında modifiye brown-brenn Gram boyama yönteminin yeri. Mikrobiyol. Bült 31:1- 11,1996.
24. Günel E, Fındık D, Çaglayan F, Topgaç Z. H. pylori seropositivity in children with recurrent abdominal pain. Tr J Med Sc 28:669-671,1998.
25. Peitz Unusch A, Tillenburg B, Stolte M, Borsch G, Labenz J. High cure rate of H. pylori infection by one-week therapy with omeprazole, metronidazole and clarithromycin despite a negative impact by metronidazole resistance. Dr. Torelli’s Helicobacter News (http:/www.oka.urban.or.jp/home/yosh iro/atnews02.html).
26. Conray MC, Korwin JD, Lozniewski A, Weber M. Resistance of H. pylori to clarithromycin and metronidazole in eastern France. Dr. Torelli’s Helicobacter News (http:/ www.oka. urban.or.jp/home/yoshiro/atnews02.ht ml).
27. Piccolomini R, Bonaventura G, Catamo G, Carbone F, Neri M. Comparative evaluation of the E test, agar dilution and broth microdilution for testing susceptibility of H. pylori strains to 20 antimicrobial agents. J Clin Microbiol 35: 1842-1846,1997.
28. Stamm WE, Stapleton AE. Approach to the patient with urinary tract infection. Infectious Diseases (Eds. ST. Gorbach, JG Barlett, and NR. Blacklow) Saunders Comp. Philadelphia, 1998, pp : 443-454.

APA	AĞEL E, DURMAZ B, TEVFİK M, AŞGIN N (2000). The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. , 143 - 146.
Chicago	AĞEL Esra,DURMAZ Bengül,TEVFİK Mehmet Refik,AŞGIN NERGİS The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. (2000): 143 - 146.
MLA	AĞEL Esra,DURMAZ Bengül,TEVFİK Mehmet Refik,AŞGIN NERGİS The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. , 2000, ss.143 - 146.
AMA	AĞEL E,DURMAZ B,TEVFİK M,AŞGIN N The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. . 2000; 143 - 146.
Vancouver	AĞEL E,DURMAZ B,TEVFİK M,AŞGIN N The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. . 2000; 143 - 146.
IEEE	AĞEL E,DURMAZ B,TEVFİK M,AŞGIN N "The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients." , ss.143 - 146, 2000.
ISNAD	AĞEL, Esra vd. "The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients". (2000), 143-146.

APA	AĞEL E, DURMAZ B, TEVFİK M, AŞGIN N (2000). The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. Turkish Journal of Medical Sciences, 30(2), 143 - 146.
Chicago	AĞEL Esra,DURMAZ Bengül,TEVFİK Mehmet Refik,AŞGIN NERGİS The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. Turkish Journal of Medical Sciences 30, no.2 (2000): 143 - 146.
MLA	AĞEL Esra,DURMAZ Bengül,TEVFİK Mehmet Refik,AŞGIN NERGİS The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. Turkish Journal of Medical Sciences, vol.30, no.2, 2000, ss.143 - 146.
AMA	AĞEL E,DURMAZ B,TEVFİK M,AŞGIN N The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. Turkish Journal of Medical Sciences. 2000; 30(2): 143 - 146.
Vancouver	AĞEL E,DURMAZ B,TEVFİK M,AŞGIN N The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients. Turkish Journal of Medical Sciences. 2000; 30(2): 143 - 146.
IEEE	AĞEL E,DURMAZ B,TEVFİK M,AŞGIN N "The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients." Turkish Journal of Medical Sciences, 30, ss.143 - 146, 2000.
ISNAD	AĞEL, Esra vd. "The isolation rate and antibiotic resistant pattern of Helicobacter pylori in dyspeptic patients". Turkish Journal of Medical Sciences 30/2 (2000), 143-146.