Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae

Kalender, Yusuf; Kalender, Suna; UZUNHİSARCIKLI, Meltem; ASLANTÜRK, AYŞE

Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae

AYŞE ASLANTÜRK, (Gazi Üniversitesi, Sağlık Hizmetleri Meslek Yüksek Okulu, Gölbaşı, Ankara, Türkiye)

Suna KALENDER, (Gazi Üniversitesi, Gazi Eğitim Fakültesi, Fen Bilgisi Eğitimi Anabilim Dalı, Ankara, Türkiye)

Meltem UZUNHİSARCIKLI, (Gazi Üniversitesi, Sağlık Hizmetleri Meslek Yüksek Okulu, Gölbaşı, Ankara, Türkiye)

Yusuf KALENDER (Gazi Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Ankara Türkiye)

Journal of the Entomological Research Society

2 0

Yıl: 2011 Cilt: 13 Sayı: 3 Sayfa Aralığı: 27 - 38 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae

Öz:

Lymantria dispar larvae are very hazardous to forests and they also cause allergic reactions in humans. In the present study, distinct concentrations of methidathion were applied to L. dispar larvae via oak leaves which were prepared by the dipping method. The data obtained was statistically appraised using probit analysis and a $LC _{50/48h}$ value for L. dispar larvae found to be 25,480 ppm. Antioxidant defense components protect insects by scavenging reactive oxygen species, leading to oxidative stress. The present study was investigated the effects of $LC _{50/48h}$ value of methidathion, on the oxidative stress indicator, malondialdehyde (MDA), and antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)] activities in midgut tissues. There were statistically significant increases in the MDA level and SOD, CAT, GPx activities in the $LC _{50/48h}$ concentration of methidathion-treated larvae compared to the control larvae. These results indicated that methidathion which is an organophosphate pesticide, causes an increase in oxidative stress and we infered that increased oxidative stress induces antioxidant defence mechanisms.

Anahtar Kelime: midgut organothiophosphate insecticides enzyme activity glutathione peroxidase antioxidants catalase methidathion superoxide dismutase oxidative stress larvae Lymantria dispar defence mechanisms

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Aebi, H., 1984. Catalase, in vitro. Methods in Enzymology. 105: 121-126.
Ahmad, S., Beilsen, M. A., Pardini, R. S.,1989. Glutathione peroxidase activity in insects: a reassessment, Archives of Insect Biochemistry and Physiology, 12: 31-39.
Ahmad S., Pardini, R. S., 1990. Mechanisms for regulating oxygen toxicity in phytophagous insects, Free Radical Biology and Medicine, 8: 401-413.
Ahmad S., Duval, D. L., Weinhold, L.C., Pardini, R.S., 1991. Cabbage looper antioxidant enzymes: tissue specificity, Insect Biochemistry, 21: 563-572.
Altuntas I., Delibas, N., Sutcu, R., 2002. The effects of organophosphate insecticide methidathion on lipid peroxidation and antioxidant enzymes in rat erythrocytes: role of Vitamins E and C. Human Experimental Toxicology, 21: 681-685.
Bagchi D., Bagchi, M., Hassoun, E. A., Stohs, S. J., 1995. In vitro and in vivo generation of reactive oxygen species. DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicology, 104: 129-140.
Bakr, R. F., Kamel, A., M., Sheba, S. A., Abdel-Haleem, D. R., 2010. A mathematical model for estimating the LC50 (or LD50) among an insect life cycle. Egyptian Academic Journal of Biological Sciences, 3(2): 75-81.
Bukowska, B., 2004. 2, 4,5-T and 2,4,5-TCP induce oxidative damage in human erytrocytes: The role of glutathione. Cell Biology International, 28: 557-563.
Buyukguzel, E., Kalender, Y., 2009. Exposure streptomycin alters oxidative and antioxidative response in larval midgut tissues of Galleria mellonella. Pesticide Biochemistry, 94: 112-118.
Buyukguzel, E., 2009. Evidence of oxidative and antioxidative responses by Galleria mellonella larvae to malathion. Journal of Economic Entomology, 102(1): 152-159.
Buyukguzel, K., 2006. Malathion induced oxidative stres in parasitoid wasp: Effect on adult emergence, longevity, fecundity, oxidative and antioxidative response of the Pimpla turionellae. Journal of Economic Entomology, 100: 1533-1541.
Cahill, M., Byrne, F. J., Gorman, K., Denholm, I., Devonshire, A. L., 1995. Pyrethroid and organophosphate resistance in tobacco whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Bulletin of Entomological Research, 85: 181-187.
Dalton T. P., Shertzer, H. G., Puga, A., 1999. Regulation of gene expression by reactive oxygen. Annual Review of Pharmacology and Toxicology, 39: 67-101.
Del Rio, D., Stewart, A. J., Pellegrini, N., 2005. A review of recent studies on malondialdeyhde as toxic molecule ad biological marker of oxidative stress. Nutrition, Metabolism and Cardiovascular Disease, 15: 316-328.
Diaz, J.H., 2005. The evolving global epidemiology, syndromic classification, management, and prevention of caterpillar envenoming. American Journal of Tropicall Medicine and Hygiene, 72(3): 347-357.
Dubovskii, I. M., Olifrenko, O. A., Glupov, V. V., 2005. Level and activities of antioxidants in intestine of larvae Galleria mellonella L. (Lepidoptera, Pyralidae) at peroral infestation by bacteria Bacillus thuringiensis ssp. galleriae, Journal of Evolutionary Biochemistry and Physiology, 41(1): 20-25.
Dubovskiy, I. M., Martemyanow, V. V., Vorontsova, Y. L., Rantala, M. J., Gryzanova, E.V., Glupov, V.V., 2008. Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut of Galleria mellonella L. larvae (Lepidoptera, Pyralidae). Comparative Biochemistry and Physiology, Part C, 148: 1-5.
Durak, D., Uzun, F. G., Kalender, S., Ogutcu, A., Uzunhisarcikli, M., Kalender, Y., 2009. Malathion-induced oxidative stres in human erythrocytes and protective effect of vitamins C and E in vitro. Environmental Toxicology, 24(3): 235-242.
Felton, G. W., Summers, C. B., 1995. Antioxidant systems in insects. Archives of Insect Biochemistry and Physiology, 29: 187-197.
Finney, D. J., 1971. Probit analysis. 3rd Edition. Cambridge University Press, Cambridge, UK. 333.
Fragoso, D. B., Guedes, R. N., Picanço, M. C., Zambolim, L., 2002. Insecticide use and organophosphate resistance in the coffee leaf miner Leucoptera coffeella (Lepidoptera: Lyonetiidae). Bulletin of Entomological Research, 92(3): 203-212.
Fridovich, I., 1978. The biology of oxygen radicals. Science, 201: 875-879.
Goertz, D., Pilarska, D., Kereselidze, M., Solter, L. F., Linde, A., 2004. Studies on the impact of two Nosema isolates from Bulgaria on the gypsy moth (Lymantria dispar L.). Journal of Invertebrate Pathology, 87: 105-113.
Gultekin, F., Ozturk, M., Akdogan, M., 2000. The effects of organophosphate insecticide chlorpyrifos-ethyl on lipid peroxidation and antioxidant enzymes (in vitro). Archives of Toxicology, 74: 533-538.
Gupta, S. C., Mishra, M., Sharma, A., Deepak Balaji, T. G. R., Kumar, R., Mishra, R. K., Chowdhuri, D. K., 2010. Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species. Ecotoxicology and Environmental Safety, 73 (6):1415-1423.
Hayes, W. J., Laws, E. R. (Eds), 1991. Handbook of pesticide toxicology. Academic Press, San Diego, New York, USA.
John, S., Kale, M., Rathore, N., Bhatnagar, D., 2001. Protective effect of vitamin E in dimethoate and malathion induced oxidative stress in rat erythrocytes. Journal of Nutritional Biochemistry, 12: 500-504.
Kalender, S., Kalender, Y., Ogutcu, A., Uzunhisarcikli, M., Durak, D., Acikgoz, F., 2004. Endosulfan-induced cardiotoxicity and free radical metabolism in rats:the protective effect of vitamin E. Toxicology, 202(3): 227-235.
Kalender, S., Ogutcu, A., Uzunhisarcikli, M., Acıkgoz, F., Durak, D., Ulusoy, Y., Kalender, Y., 2005. Diazinon-induced hepatotoxicity and protective effect of vitamin E on some biological indices and ultrastructural changes. Toxicology, 211(3): 197-206.
Kalender, S., Kalender, Y., Durak, D., Ogutcu, A., Uzunhisarcikli, M., Cevrimli, B. S., Yıldırım, M., 2007. Methyl parathion induced nephrotoxicity in male rats and protective role of vitamins C and E. Pesticides Biochemistry and Physiology, 88(2): 213-218.
Kalender, Y., Uzunhisarcikli, M., Ogutcu, A., Suludere, Z., Kalender, S., 2005. Effects of endosulfan on Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae) larvae. Folia Biologica (Krakow), 53: 3-4.
Kalender, Y., Uzunhisarcikli, M., Ogutcu, A., Acikgoz, F., Kalender, S., 2006. Effects of diazinon on pseudocholinesterase activity and haematological indices in rats: the protective role of vitamin E. Environmental Toxicology and Pharmacology, 22(1): 46-51.
Kamata, H., Hirata, H., 1999. Redox regulation of cellular signalling. Cell Signalling 11: 1-4.
Krishnan, N., Kodrik., D., 2006. Antioxidant enzymes in Spodoptera littoralis (Boisduval): Are they enhanced to protect gut tissues during oxidative stress? Journal of Insect Physiology, 52: 11–20.
Liebhold, A., Elkinton, J., Wiliams, D., Muzika, R. M., 2000. What causes outbreaks of the gypsy moth in North America? Population Ecology, 42: 257-266.
Lopez Martinez, G., Elnitsky, M. A., Benoit, J. B., LEE Jr, R. E., Denlinger, D. L., 2008. High resistance to oxidative damage in the Antarctic midge Belgica antarctica, and developmentally linked expression of genes encoding superoxide dismutase, catalase and heat shock proteins. Insect Biochemistry and Molecular Biology, 38: 796-804.
Lowry, O., Rosenbraugh, N., Farr, L., Randall, R., 1951. Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 183: 265-275.
Marklund, S., Marklund, G., 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase, European Journal of Biochemistry. 47:469-474.
Ogutcu, A., Suludere, Z., Uzunhisarcikli, M., Kalender, Y., 2005. Effects of Bacillus thuringiensis kurstaki on Malpighian tubule cells of Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae) larvae. Folia Biologica Krakow, 53(1-2): 7-11.
Ogutcu, A., Uzunhisarcikli, M., Kalender, S., Durak, D., Bayrakdar, F., Kalender, Y., 2006. The effects of organophosphate insecticide diazinon on malondialdehyde levels and myocardial cells in rat heart tissue and protective role of vitamin E. Pesticides Biochemistry and Physiology, 86(2): 93-98.
Ogutcu A., Suludere, Z., Kalender, Y., 2008. Dichlorvos-induced hepatotoxicity in rats and the protective effects of vitamins C and E. Enviromental Toxicology and Pharmacology. 26: 355-361.
Ohkawa, H., Ohishi, N., Yagi, K., 1979. Assay of lipid peroxidation in animal tissues by thiobarbituric acid reaction, Analytical Biochemistry, 95: 351-358.
Orr, W. C., Sohal, R. S., 1994. Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster. Science, 263: 1128-1130.
Paglia, D. E., Valentine, W. N., 1967. Studies on the quantitative and qualitative characterization of erythrocytes glutathione peroxides. Journal of Laboratory and Clinical Medicine, 70: 158-169.
Peric-Mataruga, V., Blagojevic, D., Spasic, M. B., Ivanovic-Hladni, M., 1997. Effect of the host plant on the oxidative defence in the midgut of Lymantria dispar L. caterpillars of different population origins. Journal of Insect Physiology, 43: 101-106.
Rael, L. T., Thomas, G. W., Craun, M. L., Curtis, C. G., Bar-or R., Bar-or, D., 2004. Lipid peroxidation and the thiobarbituric acid assay: standardization of the assay when using saturated and unsaturated fatty acids. Journal of Biochemistry and Molecular Biology, 37: 749-752.
Rausell, C., Martinez-Ramirez, A. C., Garcia Roblez, I., Real, M. D., 1999. The toxicity and physiological effects of Bacillus thuringiensis toxins and formulations on Thaumetopoea pityocampa, the pine processionary caterpillar. Pesticides Biochemistry and Physiology, 65: 44-54.
Shoba, V., Elanchezhiyan, C., Selvisabhanayakam, Hemalatha, S.,Jagadeesan, G., 2010. Evaluation of LC50 value of the phytopesticide nimbecidine on the adult male insect Sphaerodema rusticum (Heteroptera: Belostomatidae). International Journal of Recent Scientific Research. 8: 177-180.
US EPA. 1999. LC50 software program, version 1.5. Center for Exposure Assesment Modeling (CEAM). Distribution Center.
Uzun, F. G., Kalender, S., Durak, D., Demir, F., Kalender, Y., 2009. Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food and Chemical Toxicology, 47(8): 1903-1908.
Uzunhisarcikli, M., Kalender, Y., Dirican, E. K., Kalender, S., Ogutcu, A., Buyukkomurcu, F., 2007. Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E. Pesticide Biochemistry and Physiology, 87(2): 115-122.
Wu, H., Liu, J., Zhang, R., Zhang, J., Guo, Y., Ma, E., 2011. Biochemical effects of acute phoxim administration on antioxidant system and acethylcholinesterase in Oxya chinensis (Thunberg) (Orthoptera: Acrididae). Pesticide Biochemistry and Physiology, 100: 23-26.
Yamamoto, K., Teshiba, S., Shigeoka, Y., Aso, Y., Banno, Y., Fujiki, T., Katakura, Y., 2011. Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth. Insect Molecular Biology, 20 (3), 379–386.
Yamamoto, K., Teshiba, S., Shigeoka, Y., Aso, Y., Banno, Y., Fujiki, T., Katakura, Y., 2011. Characterization of an omega-class glutathione S-transferase in the stress response of the silkmoth. Insect Molecular Biology, 20 (3), 379–386.
Yang, M. L., Zhang, J. Z., Zhu, K. Y., Xuan, T., Liu, X. J., Guo, Y. P., Ma, E. B., 2009. Mechanisms of organophosphate resistance in a field population of oriental migratory locust, Locusta migratoria manilensis (Meyen). Archives of Insect Biochemistry and Physiology, 71: 3-15.

APA	ASLANTÜRK A, Kalender S, UZUNHİSARCIKLI M, Kalender Y (2011). Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. , 27 - 38.
Chicago	ASLANTÜRK AYŞE,Kalender Suna,UZUNHİSARCIKLI Meltem,Kalender Yusuf Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. (2011): 27 - 38.
MLA	ASLANTÜRK AYŞE,Kalender Suna,UZUNHİSARCIKLI Meltem,Kalender Yusuf Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. , 2011, ss.27 - 38.
AMA	ASLANTÜRK A,Kalender S,UZUNHİSARCIKLI M,Kalender Y Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. . 2011; 27 - 38.
Vancouver	ASLANTÜRK A,Kalender S,UZUNHİSARCIKLI M,Kalender Y Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. . 2011; 27 - 38.
IEEE	ASLANTÜRK A,Kalender S,UZUNHİSARCIKLI M,Kalender Y "Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae." , ss.27 - 38, 2011.
ISNAD	ASLANTÜRK, AYŞE vd. "Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae". (2011), 27-38.

APA	ASLANTÜRK A, Kalender S, UZUNHİSARCIKLI M, Kalender Y (2011). Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. Journal of the Entomological Research Society, 13(3), 27 - 38.
Chicago	ASLANTÜRK AYŞE,Kalender Suna,UZUNHİSARCIKLI Meltem,Kalender Yusuf Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. Journal of the Entomological Research Society 13, no.3 (2011): 27 - 38.
MLA	ASLANTÜRK AYŞE,Kalender Suna,UZUNHİSARCIKLI Meltem,Kalender Yusuf Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. Journal of the Entomological Research Society, vol.13, no.3, 2011, ss.27 - 38.
AMA	ASLANTÜRK A,Kalender S,UZUNHİSARCIKLI M,Kalender Y Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. Journal of the Entomological Research Society. 2011; 13(3): 27 - 38.
Vancouver	ASLANTÜRK A,Kalender S,UZUNHİSARCIKLI M,Kalender Y Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. Journal of the Entomological Research Society. 2011; 13(3): 27 - 38.
IEEE	ASLANTÜRK A,Kalender S,UZUNHİSARCIKLI M,Kalender Y "Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae." Journal of the Entomological Research Society, 13, ss.27 - 38, 2011.
ISNAD	ASLANTÜRK, AYŞE vd. "Effects of methidathion on Antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae". Journal of the Entomological Research Society 13/3 (2011), 27-38.