Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey

BALKAYA, İbrahim; ÜTÜK, Armağan Erdem; PİŞKİN, Fatma Çiğdem

Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey

Armağan Erdem ÜTÜK, (Etlik Veteriner Kontrol Merkez Araştırma Enstitüsü, Parazitoloji ve Arı Hastalıkları Laboratuvarı, Ankara, TÜRKİYE)

Fatma Çiğdem PİŞKİN, (Etlik Veteriner Kontrol Merkez Araştırma Enstitüsü, Parazitoloji ve Arı Hastalıkları Laboratuvarı, Ankara, TÜRKİYE)

İbrahim BALKAYA (Atatürk Üniversitesi, Veteriner Fakültesi, Parazitoloji Anabilim Dalı, Erzurum, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2012 Cilt: 18 Sayı: 2 Sayfa Aralığı: 303 - 307 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey

Öz:

Bu çalışma, Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (Trachurus trachurus) tespit edilen altı parazitin tür tanısınıyapmak amacıyla planlanmıştır. Nematod olarak belirlenen altı parazitten DNA ekstraksiyonu yapılmıştır. Tür tanısı, rDNA’nın ITSbölgelerinin (ITS1, 5.8 alt ünite rRNA ve ITS2) PCR ve PCR-RFLP analizleri ve mitokondrial sitokrom c oksidaz alt ünite 1 (mt-CO1) ve mt- CO2 genlerinin kısmi olarak sekanslanması ile yapılmıştır. PCR ve PCR-RFLP sonuçlarına göre tüm parazitlerin Anisakis pegreffii olduğubelirlenmiştir. Rastgele seçilen bir parazitin mt-CO1 geninin kısmi sekans sonucu A. simplex, iki parazitin mt-CO2 geninin kısmi sekanssonuçları ise A. pegreffii ile uyuşmuştur. Bu çalışma ile A. pegreffii Türkiye’de ilk kez moleküler olarak tespit edilmiştir.

Anahtar Kelime: teşhis Erzurum Trachurus trachurus Türkiye Anisakis anisakiyaz

Konular: Veterinerlik

Türkiye’nin Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (trachurus trachurus) anisakis pegreffii’nin moleküler tanısı

Öz:

This study was planned to identify six parasites detected in horse mackerels (Trachurus trachurus) sold for human consumptionin Erzurum province. DNA extraction was performed on six parasites diagnosed as nematode. Species identification was doneby PCR and PCR-RFLP analysis of ITS (ITS-1, 5.8S subunit rRNA gene and ITS-2) region of ribosomal DNA (rDNA) and partialsequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) and mt-CO2 genes. According to the PCR and PCR-RFLPresults, all parasites were identified as Anisakis pegreffii. Partial sequence of mt-CO1 gene of one randomly selected parasitewas corresponding with A. simplex and mt-CO2 genes of two parasites were corresponding with A. pegreffii. With this study, A.pegreffii was molecularly detected for the first time in Turkey.

Anahtar Kelime: Turkey Anisakis anisakiasis diagnosis Erzurum Trachurus trachurus

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÜTÜK A, PİŞKİN F, BALKAYA İ (2012). Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. , 303 - 307.
Chicago	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,BALKAYA İbrahim Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. (2012): 303 - 307.
MLA	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,BALKAYA İbrahim Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. , 2012, ss.303 - 307.
AMA	ÜTÜK A,PİŞKİN F,BALKAYA İ Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. . 2012; 303 - 307.
Vancouver	ÜTÜK A,PİŞKİN F,BALKAYA İ Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. . 2012; 303 - 307.
IEEE	ÜTÜK A,PİŞKİN F,BALKAYA İ "Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey." , ss.303 - 307, 2012.
ISNAD	ÜTÜK, Armağan Erdem vd. "Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey". (2012), 303-307.

APA	ÜTÜK A, PİŞKİN F, BALKAYA İ (2012). Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18(2), 303 - 307.
Chicago	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,BALKAYA İbrahim Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 18, no.2 (2012): 303 - 307.
MLA	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,BALKAYA İbrahim Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.18, no.2, 2012, ss.303 - 307.
AMA	ÜTÜK A,PİŞKİN F,BALKAYA İ Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2012; 18(2): 303 - 307.
Vancouver	ÜTÜK A,PİŞKİN F,BALKAYA İ Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2012; 18(2): 303 - 307.
IEEE	ÜTÜK A,PİŞKİN F,BALKAYA İ "Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18, ss.303 - 307, 2012.
ISNAD	ÜTÜK, Armağan Erdem vd. "Molecular detection of anisakis pegreffii in horse mackerels (trachurus trachurus) sold for human consumption in Erzurum province of Turkey". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 18/2 (2012), 303-307.