The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C

KULAKSIZ, Recai; ÇEBİ, Çiğdem; Akcay, Ergun

The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C

Recai KULAKSIZ, (Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, University of Kafkas, Kars, Turkey)

Çiğdem ÇEBİ, (Department of Reproduction and Artifi cial Insemination, Faculty of Veterinary Medicine, University of Ankara, Turkey)

ERGÜN AKÇAY (Department of Reproduction and Artifi cial Insemination, Faculty of Veterinary Medicine, University of Ankara, Turkey)

Turkish Journal of Veterinary and Animal Sciences

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Yıl: 2012 Cilt: 36 Sayı: 2 Sayfa Aralığı: 177 - 182 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C

Öz:

Bu çalışma, koç spermasının yağsız süt tozu (M), sodyum sitrat (SC), Tris (T) ve Bioxcell® (B) sulandırıcıları ile sulandırılıp, +4 °C de kısa süreli saklanması ve dondurulması sonrası spermatozoa motilitesi ve anormalitesi üzerine etkisinin saptanması amacıyla yapıldı. Ejakulatlar 3 Akkaraman koçundan sezon dışı dönemde suni vajen yardımıyla haft ada 2 kez alındı. Alınan ejakulatlar birleştirildikten sonra 4 eşit hacme bölünmüş ve yağsız süt tozu (M), sodyum sitrat (SC), Tris (T) ve Bioxcell® (B) sulandırıcıları ile sulandırılmıştır. Kısa süreli saklamanın 24 ve 48. saatlerinde elde edilen en yüksek spermatozoa motilitesi yağsız süt tozunda elde edildi. Saklamanın 24. saatinde, anormal spermatozoa oranı bakımından, yağsız süt tozu sulandırıcısı ile Tris ve sodyum sitrat sulandırıcı içeren gruplar karşılaştırıldığında istatistiksel olarak aradaki farklılıklar önemli bulunurken (P < 0,05), Bioxcell® ile yağsız süt tozu arasındaki farklılıklar istatistiksel açıdan önemsiz bulunmuştur. Çözdürme sonrası spermatozoa motilitesi ve total anormal spermatozoa oranı üzerine sulandırıcılar arasındaki farklılıklar önemli bulunmuştur. Süt tozu sulandırıcısında, total anormal spermatozoa oranı diğer sulandırıcılara göre önemli derecede daha düşüktür. Sonuç olarak, çözdürme ve kısa süreli saklama sonrası bazı spermatolojik parametreleri değerlendirildiğinde yağsız süt tozu sulandırıcısının diğer sulandırıcılardan daha iyi sonuçlar verdiği saptandı.

Anahtar Kelime: motilite spermatozoa dondurulmuş semen spermatosit ejakülat derecesi sodyum sitrat koç semen koruma suni tohumlama semen sulandırıcısı anormallikler koyun semen sulandırıcı katkıları morfoloji yağsız süt

Konular: Ziraat Mühendisliği

Koç spermasının kısa süreli saklanması ve dondurulmasında farklı sulandırıcıların motilite ve morfoloji üzerine etkisi

Öz:

Th is study was conducted to evaluate the eff ects on sperm motility and abnormality in ram semen extended with skimmed milk (M), sodium citrate (SC), Tris (T), and Bioxcell® (B) aft er storage in liquid form at 4 °C and in frozen form. Ejaculates were collected from 3 Akkaraman rams by artifi cial vagina twice a week during the non-breeding season. Aft er pooling, each pooled ejaculate was split into 4 equal aliquots and diluted with skimmed milk (M), sodium citrate (SC), Tris (T), and Bioxcell® (B) extenders. Sperm motility was signifi cantly higher (P < 0.05) in M compared with B, SC, and T on the fi rst and second days (48 h) of storage. With regard to the percentage of total abnormal spermatozoa, the results in M were diff erent from (P < 0.05) from those of SC and T on the fi rst day (24 h) of storage but no diff erent from B. Diff erences among extenders were found to be signifi cant post-thawing for spermatozoa motility and the percentage of total abnormal spermatozoa. Th e total abnormality of semen diluted with M was signifi cantly lower than that observed in the other extenders. Consequently, it was found that skimmed milk was better than the other extenders in terms of the sperm parameters evaluated during liquid storage and post-thaw.

Anahtar Kelime: abnormalities sheep semen diluent additives morphology skim milk motility spermatozoa frozen semen spermatocytes ejaculate volume sodium citrate rams semen preservation artificial insemination semen diluents

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KULAKSIZ R, ÇEBİ Ç, Akcay E (2012). The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. , 177 - 182.
Chicago	KULAKSIZ Recai,ÇEBİ Çiğdem,Akcay Ergun The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. (2012): 177 - 182.
MLA	KULAKSIZ Recai,ÇEBİ Çiğdem,Akcay Ergun The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. , 2012, ss.177 - 182.
AMA	KULAKSIZ R,ÇEBİ Ç,Akcay E The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. . 2012; 177 - 182.
Vancouver	KULAKSIZ R,ÇEBİ Ç,Akcay E The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. . 2012; 177 - 182.
IEEE	KULAKSIZ R,ÇEBİ Ç,Akcay E "The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C." , ss.177 - 182, 2012.
ISNAD	KULAKSIZ, Recai vd. "The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C". (2012), 177-182.

APA	KULAKSIZ R, ÇEBİ Ç, Akcay E (2012). The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Turkish Journal of Veterinary and Animal Sciences, 36(2), 177 - 182.
Chicago	KULAKSIZ Recai,ÇEBİ Çiğdem,Akcay Ergun The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Turkish Journal of Veterinary and Animal Sciences 36, no.2 (2012): 177 - 182.
MLA	KULAKSIZ Recai,ÇEBİ Çiğdem,Akcay Ergun The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Turkish Journal of Veterinary and Animal Sciences, vol.36, no.2, 2012, ss.177 - 182.
AMA	KULAKSIZ R,ÇEBİ Ç,Akcay E The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Turkish Journal of Veterinary and Animal Sciences. 2012; 36(2): 177 - 182.
Vancouver	KULAKSIZ R,ÇEBİ Ç,Akcay E The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Turkish Journal of Veterinary and Animal Sciences. 2012; 36(2): 177 - 182.
IEEE	KULAKSIZ R,ÇEBİ Ç,Akcay E "The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C." Turkish Journal of Veterinary and Animal Sciences, 36, ss.177 - 182, 2012.
ISNAD	KULAKSIZ, Recai vd. "The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C". Turkish Journal of Veterinary and Animal Sciences 36/2 (2012), 177-182.