Yıl: 2011 Cilt: 11 Sayı: 4 Sayfa Aralığı: 507 - 513 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

A Survey of viral hemorrhagic septicemia (VHS) in Turkey

Öz:
Türkiye'de viral hemorajik septisemi virüsünün (VHSV) varlığı son yıllarda rapor edilmiştir. Bu çalışmanın amacı 2007- 2009 yılları arasında, Karadeniz bölgesinde, tatlı su ve deniz balıklarında VHSV'nin varlığının araştırılmasıdır. Tarama bölgesinden 2009 yılının sonuna kadar 13 farklı deniz ve tatlı su balığı türünden toplam 5967 adet balık örneklenmiş, balık örneklerinden beş tanesi hem dokudan hem de CPE gösteren hücre kültürü süpernatantından, VHSV'nin glikoprotein geninin 587 bç'lik korunmuş bir bölgesine spesifik primerler kullanılarak yapılan PCR reaksiyonuyla pozitif bulunmuştur. Pozitif bulunan tüm örnekler yalnızca kalkan balıklarından alınmıştır. Bu beş izolatın nükleotit dizilimleri arasında %96 ila %98 homoloji olduğu görülmektedir. Yapılan filogenetik analizde tüm izolatların genogrup Ie içerisinde yer aldıkları tespit edilmiştir.
Anahtar Kelime: epidemiyoloji hastalık bulaşması nükleotit dizileri karışık enfeksiyon viral proteinler tatlısu balıkları Akdeniz hastalık tetkiki glikoprotein epidemiyolojik araştırmalar hastalık yaygınlığı Karadeniz Türkiye filogeni genler deniz balıkları polimeraz zincir reaksiyonu

Konular: Balıkçılık Zooloji

Türkiye'de viral hemorajik septisemi (VHS) Taraması

Öz:
Existence of viral hemorrhagic septicemia virus (VHSV) has been recently reported in Turkey. A total of 5967 marine and freshwater fish were sampled to detect VHSV between 2007 and 2009 in the Black Sea region of Turkey. Of the 5967 fish, five fish tested positive by PCR reactions of tissue filtrates and supernatants of cell cultures showing CPE indicated by a specific 587 bp fragments from the glycoprotein (G) gene of VHSV. All the VHSV positive fish were turbots and none of the other fish species was VHSV positive. The nucleotide sequences among five isolates had shown 96% to 99% homology. In the phylogenetic study, all the new isolates were genogroup 1e. In this study, it was also discovered that an IPNV-VHSV mixed infected wild turbot.
Anahtar Kelime: disease surveys glycoproteins epidemiological surveys disease prevalence Black Sea Turkey phylogeny genes marine fishes polymerase chain reaction epidemiology disease transmission nucleotide sequences mixed infections viral proteins freshwater fishes Mediterranean Sea

Konular: Balıkçılık Zooloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA IŞIDAN H, BOLAT Y (2011). A Survey of viral hemorrhagic septicemia (VHS) in Turkey. , 507 - 513.
Chicago IŞIDAN Hakan,BOLAT Yusuf A Survey of viral hemorrhagic septicemia (VHS) in Turkey. (2011): 507 - 513.
MLA IŞIDAN Hakan,BOLAT Yusuf A Survey of viral hemorrhagic septicemia (VHS) in Turkey. , 2011, ss.507 - 513.
AMA IŞIDAN H,BOLAT Y A Survey of viral hemorrhagic septicemia (VHS) in Turkey. . 2011; 507 - 513.
Vancouver IŞIDAN H,BOLAT Y A Survey of viral hemorrhagic septicemia (VHS) in Turkey. . 2011; 507 - 513.
IEEE IŞIDAN H,BOLAT Y "A Survey of viral hemorrhagic septicemia (VHS) in Turkey." , ss.507 - 513, 2011.
ISNAD IŞIDAN, Hakan - BOLAT, Yusuf. "A Survey of viral hemorrhagic septicemia (VHS) in Turkey". (2011), 507-513.
APA IŞIDAN H, BOLAT Y (2011). A Survey of viral hemorrhagic septicemia (VHS) in Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 11(4), 507 - 513.
Chicago IŞIDAN Hakan,BOLAT Yusuf A Survey of viral hemorrhagic septicemia (VHS) in Turkey. Turkish Journal of Fisheries and Aquatic Sciences 11, no.4 (2011): 507 - 513.
MLA IŞIDAN Hakan,BOLAT Yusuf A Survey of viral hemorrhagic septicemia (VHS) in Turkey. Turkish Journal of Fisheries and Aquatic Sciences, vol.11, no.4, 2011, ss.507 - 513.
AMA IŞIDAN H,BOLAT Y A Survey of viral hemorrhagic septicemia (VHS) in Turkey. Turkish Journal of Fisheries and Aquatic Sciences. 2011; 11(4): 507 - 513.
Vancouver IŞIDAN H,BOLAT Y A Survey of viral hemorrhagic septicemia (VHS) in Turkey. Turkish Journal of Fisheries and Aquatic Sciences. 2011; 11(4): 507 - 513.
IEEE IŞIDAN H,BOLAT Y "A Survey of viral hemorrhagic septicemia (VHS) in Turkey." Turkish Journal of Fisheries and Aquatic Sciences, 11, ss.507 - 513, 2011.
ISNAD IŞIDAN, Hakan - BOLAT, Yusuf. "A Survey of viral hemorrhagic septicemia (VHS) in Turkey". Turkish Journal of Fisheries and Aquatic Sciences 11/4 (2011), 507-513.