Yıl: 2012 Cilt: 5 Sayı: 1 Sayfa Aralığı: 1 - 4 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Time dependent cytotoxic role of homalothecium sericeum extracts on glioma

Öz:
Bryofitler 400 yıldan fazla bir süredir Çin, Avrupa ve Kuzey Amerika’da bitkisel ilaç olarak kullanılmaktadır.Bryofitlerin prokaryot, mantar türleri üzerinde antibiyotik ve farklı kanser hücreleri üzerinde antikanser etkinlikgösterdiği konusunda veriler bulunmaktadır. Bu çalışmanın amacı, bir Bryofit olan Homalothecium sericeum’un sıçanglioma (C6) hücreleri üzerinde 48 saatlik sitotoksik etkinliğini araştırmaktır. İlk olarak, iki farklı özüt elde etmeyöntemiyle aseton ve A özütlerini elde ettik. C6 hücreleri 96 kuyucuklu kültür kaplarına ekilerek (2x104hücre/kuyucuk)24 saat inkübe edildi. İnkübasyon süresinin ardından kuyucuklara özütlerin 0.17, 1.7, 17, 85 ve 170 µg/mLkonsantrasyonları eklenerek 48 saat muamele edildi. Sitotoksisite, MTT [3-(4,5-dimetiltiazol-2yl)- 2,5- difeniltetrazolyum bromid] yöntemi ile belirlendi. 48 saat sonunda aseton özütünün 0.17, 1.7 ve 17 µg/mLkonsantrasyonları C6 hücre canlılığını değiştirmezken, 85 ve 170 µg/mL konsantrasyonları hücre canlılığını sırası ile%16 ve %36 oranında azalttı (p<0.001). A özütünün 0.17 µg/mL konsantrasyonu hücre canlılığını 48 saat sonundadeğiştirmezken, 1.7, 17 (p<0.01), 85 and 170 (p<0.001) µg/mL konsantrasyonları sırası ile % 6, 8, 24 ve 33 oranındaazaltmıştır. H. sericeum’un aseton ve A özütü glioma hücreleri üzerinde orta ve benzer bir şekilde doza bağımlısitotoksik etki göstermiştir. Bu özütlerin içeriği hakkında yapılacak daha kapsamlı çalışmalara ihtiyaç duyulmaktadır.
Anahtar Kelime: Homalothecium sericeum tıbbi bitkiler neoplazma gliom bitki özütü sıçan sitotoksisite

Konular: Biyoloji

Homalothecium sericeum özütlerinin zamana bağlı olarak glioma hücrelerinin çoğalması üzerindeki etkileri

Öz:
ryophytes have been used as medicinal plants for more than 400 years in China, Europe and North America.There is also evidence confirming the antibiotic and anticancer activity of Bryophytes against, prokaryotes, fungi anddifferent cancer cells. The purpose of the current study was to investigate cytotoxic property of Homalotheciumsericeum (hedw.) schimp., which is a bryophyte, extracts on rat glioma (C6) cells for 48 hrs, in vitro. We first collectedtwo different (acetone and A) extracts from H. sericeum by two different extraction processes. C6 cells were seeded in96 well plates (2x104cells/well) and incubated for 24 hrs. Following this incubation period, the medium was replacedwith only medium (control) or medium with extracts at concentrations of 0.17, 1.7, 17, 85 or 170 µg/mL for 48 hrs.Cytotoxicity was determined by using 3-(4,5-dimethylthiazol-2yl)- 2,5-diphenyltetrazolium bromide (MTT)colorimetric assay. Acetone extract of H. sericeum at 0.17, 1.7 and 17 µg/mL concentrations did not change the survivalrate of C6, but 85 and 170 µg/mL inhibited about 16 % and 36 % after 48 hr (p<0.001), respectively. Extract A atconcentration of 0.17 µg/mL did not also affect C6 viability, but 1.7, 17 (p<0.01), 85 and 170 (p<0.001) µg/mLdecreased C6 cell viability by 6, 8, 24 and 33 % for 48 hr, respectively. Acetone and A extracts of H. sericeum showeda moderate but similar dose dependent cytotoxicity on C6. Further studies are needed to clarify the content of theseextracts.
Anahtar Kelime: rats cytotoxicity Homalothecium sericeum medicinal plants neoplasms glioma plant extracts

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ÖZTOPCU VATAN A, kabadere s, UYAR R, SAVAROĞLU F, KUŞ G (2012). Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. , 1 - 4.
Chicago ÖZTOPCU VATAN AYŞE PINAR,kabadere selda,UYAR Ruhi,SAVAROĞLU Filiz,KUŞ Gökhan Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. (2012): 1 - 4.
MLA ÖZTOPCU VATAN AYŞE PINAR,kabadere selda,UYAR Ruhi,SAVAROĞLU Filiz,KUŞ Gökhan Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. , 2012, ss.1 - 4.
AMA ÖZTOPCU VATAN A,kabadere s,UYAR R,SAVAROĞLU F,KUŞ G Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. . 2012; 1 - 4.
Vancouver ÖZTOPCU VATAN A,kabadere s,UYAR R,SAVAROĞLU F,KUŞ G Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. . 2012; 1 - 4.
IEEE ÖZTOPCU VATAN A,kabadere s,UYAR R,SAVAROĞLU F,KUŞ G "Time dependent cytotoxic role of homalothecium sericeum extracts on glioma." , ss.1 - 4, 2012.
ISNAD ÖZTOPCU VATAN, AYŞE PINAR vd. "Time dependent cytotoxic role of homalothecium sericeum extracts on glioma". (2012), 1-4.
APA ÖZTOPCU VATAN A, kabadere s, UYAR R, SAVAROĞLU F, KUŞ G (2012). Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. Biological Diversity and Conservation, 5(1), 1 - 4.
Chicago ÖZTOPCU VATAN AYŞE PINAR,kabadere selda,UYAR Ruhi,SAVAROĞLU Filiz,KUŞ Gökhan Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. Biological Diversity and Conservation 5, no.1 (2012): 1 - 4.
MLA ÖZTOPCU VATAN AYŞE PINAR,kabadere selda,UYAR Ruhi,SAVAROĞLU Filiz,KUŞ Gökhan Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. Biological Diversity and Conservation, vol.5, no.1, 2012, ss.1 - 4.
AMA ÖZTOPCU VATAN A,kabadere s,UYAR R,SAVAROĞLU F,KUŞ G Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. Biological Diversity and Conservation. 2012; 5(1): 1 - 4.
Vancouver ÖZTOPCU VATAN A,kabadere s,UYAR R,SAVAROĞLU F,KUŞ G Time dependent cytotoxic role of homalothecium sericeum extracts on glioma. Biological Diversity and Conservation. 2012; 5(1): 1 - 4.
IEEE ÖZTOPCU VATAN A,kabadere s,UYAR R,SAVAROĞLU F,KUŞ G "Time dependent cytotoxic role of homalothecium sericeum extracts on glioma." Biological Diversity and Conservation, 5, ss.1 - 4, 2012.
ISNAD ÖZTOPCU VATAN, AYŞE PINAR vd. "Time dependent cytotoxic role of homalothecium sericeum extracts on glioma". Biological Diversity and Conservation 5/1 (2012), 1-4.