Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2)

GEDİK TUNCA, Sedef; CAMCI YALIM, İrem; AVİCAN, Ümmehan; DORUK, TUGRUL

Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2)

İrem CAMCI YALIM, (Gebze Yüksek Teknoloji Enstitüsü, Moleküler Biyoloji ve Genetik Bölümü, Kocaeli, Türkiye)

Tuğrul DORUK, (Gebze Yüksek Teknoloji Enstitüsü, Moleküler Biyoloji ve Genetik Bölümü, Kocaeli, Türkiye)

Ümmehan AVİCAN, (Gebze Yüksek Teknoloji Enstitüsü, Moleküler Biyoloji ve Genetik Bölümü, Kocaeli, Türkiye)

Sedef GEDİK TUNCA (Gebze Yüksek Teknoloji Enstitüsü, Moleküler Biyoloji ve Genetik Bölümü, Kocaeli, Türkiye)

Turkish Journal of Biology

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Yıl: 2012 Cilt: 36 Sayı: 4 Sayfa Aralığı: 373 - 380 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2)

Öz:

Polyphosphate, which is synthesized by the enzyme polyP kinase (PPK), is an important energy and phosphate storage polymer that plays a crucial role in the regulation of adaptive responses of cells to physical and chemical stresses. In this work, the polyphosphate kinase gene (ppk) of Streptomyces coelicolor was deleted, and the eff ect of this mutation on actinorhodin and undecylprodigiosin biosynthesis was investigated. Deletion of the ppk gene had a stimulatory eff ect on actinorhodin production; the mutant strain produced about 5 times more antibiotic compared to the wild type strain at 120 h of growth. There was no difference in undecylprodigiosin production between the mutant and wild-type strains. In the presence of a selective antibiotic, the mutant strain could grow only on rich medium and could not sporulate effectively. Moreover, while the wild type strain was resistant, the mutant strain was sensitive to H2O2 in the conditions tested. Mutant characters were complemented by the ppk gene, which is cloned on a high copy number plasmid.

Anahtar Kelime: antibiotics bacteria polyphosphate kinase mutants polyphosphates genes production hydrogen peroxide Streptomyces coelicolor

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	CAMCI YALIM İ, DORUK T, AVİCAN Ü, GEDİK TUNCA S (2012). Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). , 373 - 380.
Chicago	CAMCI YALIM İrem,DORUK TUGRUL,AVİCAN Ümmehan,GEDİK TUNCA Sedef Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). (2012): 373 - 380.
MLA	CAMCI YALIM İrem,DORUK TUGRUL,AVİCAN Ümmehan,GEDİK TUNCA Sedef Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). , 2012, ss.373 - 380.
AMA	CAMCI YALIM İ,DORUK T,AVİCAN Ü,GEDİK TUNCA S Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). . 2012; 373 - 380.
Vancouver	CAMCI YALIM İ,DORUK T,AVİCAN Ü,GEDİK TUNCA S Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). . 2012; 373 - 380.
IEEE	CAMCI YALIM İ,DORUK T,AVİCAN Ü,GEDİK TUNCA S "Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2)." , ss.373 - 380, 2012.
ISNAD	CAMCI YALIM, İrem vd. "Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2)". (2012), 373-380.

APA	CAMCI YALIM İ, DORUK T, AVİCAN Ü, GEDİK TUNCA S (2012). Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). Turkish Journal of Biology, 36(4), 373 - 380.
Chicago	CAMCI YALIM İrem,DORUK TUGRUL,AVİCAN Ümmehan,GEDİK TUNCA Sedef Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). Turkish Journal of Biology 36, no.4 (2012): 373 - 380.
MLA	CAMCI YALIM İrem,DORUK TUGRUL,AVİCAN Ümmehan,GEDİK TUNCA Sedef Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). Turkish Journal of Biology, vol.36, no.4, 2012, ss.373 - 380.
AMA	CAMCI YALIM İ,DORUK T,AVİCAN Ü,GEDİK TUNCA S Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). Turkish Journal of Biology. 2012; 36(4): 373 - 380.
Vancouver	CAMCI YALIM İ,DORUK T,AVİCAN Ü,GEDİK TUNCA S Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2). Turkish Journal of Biology. 2012; 36(4): 373 - 380.
IEEE	CAMCI YALIM İ,DORUK T,AVİCAN Ü,GEDİK TUNCA S "Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2)." Turkish Journal of Biology, 36, ss.373 - 380, 2012.
ISNAD	CAMCI YALIM, İrem vd. "Deletion of polyphosphate kinase gene (ppk) has a stimulatory effect on actinorhodin production by Streptomyces coelicolor A3(2)". Turkish Journal of Biology 36/4 (2012), 373-380.