Yıl: 2012 Cilt: 36 Sayı: 4 Sayfa Aralığı: 394 - 404 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain

Öz:
The effect of orange peel concentration, HCl concentration, incubation time and temperature, and inoculum size on the spore count and activity of polygalacturonase (PG) enzyme produced from Aspergillus sojae M3 by solidstate fermentation was screened using 2k factorial design. Orange peel and HCl concentrations and incubation time were significant factors affecting the responses. Optimum conditions favoring both PG and spore production from Aspergillus sojae M3 were determined as 2% orange peel and 50 mM HCl concentrations at 22 °C and 4.3 days of incubation. An overlay plot was constructed for use as a practical chart for production of high enzyme activity (>35.0 U/g substrate) and spore count (9.0 × 108 to 2.0 × 109 spore/mL) by superimposing the contours of PG activity and spore count responses. The accuracy and reliability of the constructed models on the responses was validated with the maximum calculated error rate between the predicted and actual activities at 14.1% and 22.4%, respectively.
Anahtar Kelime: orange peel Aspergillus sojae response surface methodology mutants enzyme activity fermentation products polygalacturonase peel fermentation enzymes spores

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. G ummadi S, Manoj N, Kumar DS. Structural and biochemical properties of pectinases. In: Polaina J, MacCabe AP. eds. Industrial Enzymes. Springer; 2007: pp. 99-115.
  • 2. S ingh S, Ramakrishna M, Appu Rao A. Optimisation of downstream processing parameters for the recovery of pectinase from the fermented bran of Aspergillus carbonarius. Process Biochem 35: 411-417, 1999.
  • 3. J ayani RS, Saxena S, Gupta R. Microbial pectinolytic enzymes: a review. Process Biochem 40: 2931-2944, 2005.
  • 4. Das B, Chakraborty A, Ghosh S et al. Studies on the eff ect of pH and carbon sources on enzyme activities of some pectinolytic bacteria isolated from jute retting water. Turk J Biol 35: 671- 678, 2011.
  • 5. Rajan A, Nair AJ. A comparative study on alkaline lipase production by a newly isolated Aspergillus fumigatus MTCC 9657 in submerged and solid-state fermentation using economically and industrially feasible substrate. Turk J Biol 35: 569-574, 2011.
  • 6. P andey A. Solid-state fermentation. Biochem Eng J 13: 81-84, 2003.
  • 7. R aghavarao K, Ranganathan T, Karanth N. Some engineering aspects of solid-state fermentation. Biochem Eng J 13: 127-135, 2003.
  • 8. K rishna C. Solid-state fermentation systems-an overview. Crit Rev Biotechnol 25: 1-30, 2005.
  • 9. B otella C, Ory I, Webb C et al. Hydrolytic enzyme production by Aspergillus awamori on grape pomace. Biochem Eng J 26: 100-106, 2005.
  • 10. P hutela U, Dhuna V, Sandhu S et al. Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels. Braz J Microbiol 36: 63-69, 2005.
  • 11. Z heng Z, Shetty K. Solid state production of polygalacturonase by Lentinus edodes using fruit processing wastes. Process Biochem 35: 825-830, 2000.
  • 12. C astilho L, Medronho R, Alves T. Production and extraction of pectinases obtained by solid state fermentation of agroindustrial residues with Aspergillus niger. Bioresour Technol 71: 45-50, 2000.
  • 13. P atil S, Dayanand A. Optimization of process for the production of fungal pectinases from deseeded sunfl ower head in submerged and solid-state conditions. Bioresour Technol 97: 2340-2344, 2006.
  • 14. B landino A, Iqbalsyah T, Pandiella S et al. Polygalacturonase production by Aspergillus awamori on wheat in solid-state fermentation. Appl Microbiol Biotechnol 58: 164-169, 2002.
  • 15. E l-Sheekh M, Ismail A, El-Abd M et al. Eff ective technological pectinases by Aspergillus carneus NRC1 utilizing the Egyptian orange juice industry scraps. Int Biodeterior Biodegrad 63: 12- 18, 2009.
  • 16. H ang Y, Woodams E. Production of fungal polygalacturonase from apple pomace. Lebensmittel-Wissenschaft und- Technologie 27: 194-196, 1994.
  • 17. F reitas P, Martin N, Silva D et al. Production and partial characterization of polygalacturonases produced by thermophilic Monascus sp N8 and by thermotolerant Aspergillus sp N12 on solid-state fermentation. Braz J Microbiol 37: 302-306, 2006.
  • 18. I smail A. Utilization of orange peels for the production of multienzyme complexes by some fungal strains. Process Biochem 31: 645-650, 1996.
  • 19. United States Department of Agriculture. Production, Supply and Distribution Online Database. Oranges, Fresh: Production, Supply and Distribution in Selected Countries. http://www. usda.gov, 2010.
  • 20. Braddock RJ. Handbook of Citrus By-Products and Processing Technology. John Wiley & Sons. New York; 1999.
  • 21. R amachandran S, Larroche C, Pandey A. Production of spores. In: Pandey A, Larroche C, Soccol CR. eds. Current Developments in Solid-State Fermentation. Springer; 2008: pp. 230-252.
  • 22. D e Nicolás-Santiago S, Regalado-González C, García- Almendárez B et al. Physiological, morphological, and mannanase production studies on Aspergillus niger uam-gs1 mutants. Electron J Biotechnol 9: 51-60, 2006.
  • 23. Gögus N, Tarı C, Oncü S et al. Relationship between morphology, rheology and polygalacturonase production by Aspergillus sojae ATCC 20235 in submerged cultures. Biochem Eng J 32: 171-178, 2006.
  • 24. P anda T, Naidu G, Sinha J. Multiresponse analysis of microbiological parameters affecting the production of pectolytic enzymes by Aspergillus niger: a statistical view. Process Biochem 35: 187-195, 1999.
  • 25. M ontgomery D. Design and Analysis of Experiments. John Wiley & Sons. New York; 2001.
  • 26. S hivakumar P, Nand K. Anaerobic degradation of pectin by mixed consortia and optimization of fermentation parameters for higher pectinase activity. Lett Appl Microbiol 20: 117-119, 1995.
  • 27. B hargav S, Panda B, Ali M et al. Solid-state fermentation: an overview. Chem Biochem Eng Q 22: 49-70, 2008.
  • 28. U stok FI, Tari C, Gogus N. Solid-state production of polygalacturonase by Aspergillus sojae ATCC 20235. J Biotechnol 127: 322-334, 2007.
  • 29. M artin N, Souza S, Silva R et al. Pectinase production by fungal strains in solid-state fermentation using agro-industrial bioproduct. Braz Arch Biol Tech 47: 813-819, 2004.
  • 30. S ilva D, Martins E, Silva R et al. Pectinase production by Penicillium viridicatum RFC3 by solid state fermentation using agricultural wastes and agro-industrial by-products. Braz J Microbiol 33: 318-324, 2002.
  • 31. Taşkın E, Eltem R. The enhancement of polygalacturonase and polymethylgalacturonase production on solid-state conditions by Aspergillus foetidus. Food Biotechnol 22: 203-217, 2008.
  • 32. Patil S, Dayanand A. Exploration of regional agrowastes for the production of pectinase by Aspergillus niger. Food Technol Biotechnol 44: 289-292, 2006.
  • 33. N ava I, Gaime-Perraud I, Huerta-Ochoa S et al. Penicillium commune spore production in solid-state fermentation of coffee pulp at laboratory scale and in a helical ribbons rotating reactor. J Chem Technol Biotechnol 81: 1760-1766, 2006.
  • 34. L arroche C, Gros J. Strategies for spore production by Penicillium roquefortii using solid state fermentation techniques. Process Biochem 24: 97-103, 1989.
APA DEMİR H, GÖĞÜŞ BAĞIŞ N, TARI C, HEERD D, LAHORE M (2012). Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. , 394 - 404.
Chicago DEMİR Hande,GÖĞÜŞ BAĞIŞ NİHAN,TARI Canan,HEERD Doreen,LAHORE Marcelo Fernandez Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. (2012): 394 - 404.
MLA DEMİR Hande,GÖĞÜŞ BAĞIŞ NİHAN,TARI Canan,HEERD Doreen,LAHORE Marcelo Fernandez Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. , 2012, ss.394 - 404.
AMA DEMİR H,GÖĞÜŞ BAĞIŞ N,TARI C,HEERD D,LAHORE M Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. . 2012; 394 - 404.
Vancouver DEMİR H,GÖĞÜŞ BAĞIŞ N,TARI C,HEERD D,LAHORE M Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. . 2012; 394 - 404.
IEEE DEMİR H,GÖĞÜŞ BAĞIŞ N,TARI C,HEERD D,LAHORE M "Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain." , ss.394 - 404, 2012.
ISNAD DEMİR, Hande vd. "Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain". (2012), 394-404.
APA DEMİR H, GÖĞÜŞ BAĞIŞ N, TARI C, HEERD D, LAHORE M (2012). Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. Turkish Journal of Biology, 36(4), 394 - 404.
Chicago DEMİR Hande,GÖĞÜŞ BAĞIŞ NİHAN,TARI Canan,HEERD Doreen,LAHORE Marcelo Fernandez Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. Turkish Journal of Biology 36, no.4 (2012): 394 - 404.
MLA DEMİR Hande,GÖĞÜŞ BAĞIŞ NİHAN,TARI Canan,HEERD Doreen,LAHORE Marcelo Fernandez Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. Turkish Journal of Biology, vol.36, no.4, 2012, ss.394 - 404.
AMA DEMİR H,GÖĞÜŞ BAĞIŞ N,TARI C,HEERD D,LAHORE M Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. Turkish Journal of Biology. 2012; 36(4): 394 - 404.
Vancouver DEMİR H,GÖĞÜŞ BAĞIŞ N,TARI C,HEERD D,LAHORE M Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain. Turkish Journal of Biology. 2012; 36(4): 394 - 404.
IEEE DEMİR H,GÖĞÜŞ BAĞIŞ N,TARI C,HEERD D,LAHORE M "Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain." Turkish Journal of Biology, 36, ss.394 - 404, 2012.
ISNAD DEMİR, Hande vd. "Optimization of the process parameters for the utilization of orange peel to produce polygalacturonase by solid-state fermentation from an Aspergillus sojae mutant strain". Turkish Journal of Biology 36/4 (2012), 394-404.