Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats

GENÇ, Habibe; SAYGILI, Seha; SEYMEN, HAKKI OKTAY; Ayan, Fadıl; Erdamar, Sibel; Uzun, Hafize; SALİHOĞLU, Ziya; Aytac, Erman; SEYMEN, Pınar; Sozer, Volkan; ALTUĞ, Tuncay; Karaca, Cetin; BAYRAK, İbrahim

Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats

Fadıl AYAN, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, İstanbul, Türkiye)

Erman AYTAÇ, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, İstanbul, Türkiye)

Seha SAYGILI, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Fizyoloji Anabilim Dalı, İstanbul, Türkiye)

Habibe GENÇ, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Biyokimya Anabilim Dalı, İstanbul)

Çetin KARACA, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Deney Hayvanları Araştırma Laboratuvarı, İstanbul, Türkiye)

İbrahim BAYRAK, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, Geriatri Bilim Dalı, İstanbul, Türkiye)

Hafize UZUN, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Biyokimya Anabilim Dalı, İstanbul)

Ziya SALİHOĞLU, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Anestezi Anabilim Dalı, İstanbul, Türkiye)

Sibel ERDAMAR, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul, Türkiye)

Volkan SÖZER, (Yıldız Teknik Üniversitesi, Biyokimya Bölümü, İstanbul, Türkiye)

Tuncay ALTUĞ, (Bilim Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji ve Genetik Anabilim Dalı, İstanbul, Türkiye)

Pınar SEYMEN, (Sağlık Bakanlığı, Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, İç Hastalıkları Kliniği, İstanbul, Türkiye)

Hakkı Oktay SEYMEN, (İstanbul Üniversitesi, Cerrahpaşa Tıp Fakültesi, Fizyoloji Anabilim Dalı, İstanbul, Türkiye)

Diğer Yazarlar

Turkish Journal of Gastroenterology

2 2

Yıl: 2011 Cilt: 22 Sayı: 4 Sayfa Aralığı: 408 - 413 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats

Öz:

Amaç: Parsiyel hepatektomi karaciğerin kitlesel lezyonlarının tedavisinde uygulanmaktadır. Likopen bir karotenoittir ve birçok fizyolojik mekanizmanın içinde yer alır. Bu çalışmada, sıçanlarda parsiyel hepatektomi sonrası likopen uygulamasının oksidan ve antioksidan parametreler, kalan karaciğer histolojisi ve plazma nitrik oksit düzeyi üzerine etkileri değerlendirildi. Yöntem: Otuz adet Wistar albino yetişkin erkek sıçan rastlantısal olarak üç eşit gruba ayıldı: Sham grubu, parsiyel hepatektomi grubu, likopen verilen parsiyel hepatektomi grubu. Likopen (4 mg/kg) zeytin yağında çözüldü ve hepatektomi öncesi altı hafta ve hepatektomi sonrası bir hafta gavaj ile transoral olarak (0,1 ml) verildi. Doku ve kan örnekleri hepatektomiden bir hafta sonra alındı. Bulgular: Likopen verilen parsiyel hepatektomi grubunun plazma malondialdehit (p<0.001) ve nitrik oksit (p<0.05) seviyeleri parsiyel hepatektomi grubundan anlamlı derecede yüksekti. Likopen verilen parsiyel hepatektomi grubunun eritrosit içi glutatyon (p<0.001), plazma (p<0.001) ve karaciğer dokusu Cu-Zn (p<0.05) süper oksit dismutaz seviyeleri parsiyel hepatektomi grubundan anlamlı derecede düşüktü. Sonuç: Parsiyel hepatektomi sonrası likopen uygulaması oksidatif stresi arttırarak zararlı olabilir.

Anahtar Kelime:

Konular: Cerrahi

Sıçanlarda parsiyel hepatektomi sonrası likopen uygulamasının oksidatif stres ve kalan karaciğer histolojisi üzerine etkileri

Öz:

Background/aims: Partial hepatectomy is performed for the treatment of mass lesions in the liver. Lycopene, which is a carotenoid, is present in various physiologic processes. In this study, the effects of lycopene administration in partially hepatectomized rats were evaluated by assessing various oxidant/antioxidant parameters, remnant liver histology and plasma nitric oxide levels. Methods: Thirty Wistar albino adult male rats were randomly divided into three equal groups as: Sham, Partial Hepatectomy and Lycopene-Administered + Partial Hepatectomy groups. Lycopene (4 mg/kg), which was dissolved in olive oil, was given to the rats per orally (via gavage tube) (0.1 ml) every day for 6 weeks before partial hepatectomy and for one week after partial hepatectomy. Tissue and blood samples were collected one week after partial hepatectomy. Results: Plasma malondialdehyde (p<0.001) and nitric oxide (p<0.05) levels in the lycopene-administered + partial hepatectomy group were significantly higher than in the partial hepatectomy group. Intraerythrocytic glutathione (p<0.001), plasma (p<0.001) and liver tissue Cu-Zn (p<0.05) superoxide dismutase levels of the lycopene-administered + partial hepatectomy group were significantly lower than in the partial hepatectomy group. Conclusions: Lycopene administration could be harmful by increasing oxidative stress after partial hepatectomy.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Bedirli A, Kerem M, Pasaoglu H, et al. Effects of ischemic preconditioning on regenerative capacity of hepatocyte in the ischemically damaged rat livers. J Surg Res 2005; 125: 42-8.
2. Michalopouos GK, DeFrances MC. Liver regeneration. Science 1997; 276: 60-6.
3. Webber EM, FitzGerald MJ, Brown PI, et al. Transforming growth factor-alpha expression during liver regeneration after partial hepatectomy and toxic injury, and potential interactions between transforming growth factor-alpha and hepatocyte growth factor. Hepatology 1993; 18: 142231.
4. Gavino VC, Dillard CJ, Tappel AL. Effect of dietary vitamin E and Santoquin on regenerating rat liver. Life Sci 1985; 36: 1771-7.
5. Gerster H. The potential role of lycopene for human health. J Am Coll Nutr 1997; 16: 109-26.
6. Waynforth HB, Flecknell PA. Hepatectomy in experimental and surgical technique in the rat. 2nd ed. London: Academic Press Ltd, 1992; 241-8.
7. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963; 51: 882-8.
8. Fairbanks V, Klee GG. Biochemical aspects of hematology. In: Tietz NW, ed. Textbook of clinical chemistry. Philadelphia: W.B. Saunders Company, 1986; 1532-4.
9. Sun Y, Oberly LW, Ying LA. Simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497–500.
10. Angel MF, Ramasastry SS, Swartz WM, et al. The critical relationship between free radicals and degrees of ischemia: evidence for tissue intolerance of marginal perfusion. Plast Reconstr Surg 1988; 81: 233-9.
11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenol reagent. J Biol Chem 1951; 193: 265-75.
12. Green LC, Wagner DA, Glogowski J, et al. Analysis of nitrate, nitrite and (15 N) nitrate in biological fluids. Anal Biochem 1982; 126: 131-8.
13. Karavias DD, Tepetes K, Karatzas T, et al. Liver resection for metastatic non-colorectal non-neuroendocrine hepatic neoplasms. Eur J Surg Oncol 2002; 28: 135-9.
14. Weitz J, Blumgart LH, Fong Y, et al. Partial hepatectomy for metastases from noncolorectal, nonneuroendocrine carcinoma. Ann Surg 2005; 241: 269-76.
15. Hemming AW, Sielaff TD, Gallinger S, et al. Hepatic resection of noncolorectal nonneuroendocrine metastases. Liver Transpl 2000; 6: 97-101.
16. Helling TS. Liver failure following partial hepatectomy. HPB (Oxford) 2006; 8: 165-74.
17. Guerrieri F, Vendemiale G, Grattagliano I, et al. Mitochondrial oxidative alterations following partial hepatectomy. Free Radic Biol Med 1999; 26: 34-41.
18. Carnovale CE, Scapini C, Alvarez ML, et al. Nitric oxide release and enhancement of lipid peroxidation in the regenerating rat liver. J Hepatol 2000; 32: 798-804.
19. Alexandris IH, Assimakopoulos SF, Vagianos CE, et al. Oxidative state in intestine and liver after partial hepatectomy in rats. Effect of bombesin and neurotensin. Clin Biochem 2004; 37: 350-6.
20. Hernandez-Munoz R, Sanchez-Sevilla L, Martinez-Gomez A, Dent MA. Changes in mitochondrial adenine nucleotides and in permeability transition in two models of rat liver regeneration. Hepatology 2003; 37: 842-51.
21. Cheeseman KH, Collins M, Maddix S, et al. Lipid peroxidation in regenerating rat liver. FEBS Lett 1986; 209: 191-6.
22. Gerster H. The potential role of lycopene for human health. J Am Coll Nutr 1997; 16: 109-26.
23. Jamshidzadeh A, Baghban M, Azarpira N, et al. Effects of tomato extract on oxidative stress induced toxicity in different organs of rats. Food Chem Toxicol 2008; Epub ahead of print.
24. Moreira EA, Fagundes RL, Filho DW, et al. Effects of diet energy level and tomato powder consumption on antioxidant status in rats. Clin Nutr 2005; 24: 1038-46.
25. Slater TF, Cheeseman KH, Benedetto C, et al. Studies on the hyperplasia (“regeneration”) of the rat liver following partial hepatectomy. Changes in lipid peroxidation and general biochemical aspects. Biochem J 1990; 265: 51-9.
26. Liu CC, Huang CC, Lin WT, et al. Lycopene supplementation attenuated xanthine oxidase and myeloperoxidase activities in skeletal muscle tissues of rats after exhaustive exercise. Br J Nutr 2005; 94: 595-601.
27. Breinholt V, Lauridsen ST, Daneshvar B, Jakobsen J. Dose-response effects of lycopene on selected drug-metabolizing and antioxidant enzymes in the rat. Cancer Lett 2000; 154: 201-10.
28. Yilmaz S, Atessahin A, Sahna E, et al. Protective effect of lycopene on adriamycin-induced cardiotoxicity and nephrotoxicity. Toxicology 2006; 218: 164-71.
29. Paiva SA, Novo R, Matsubara BB, et al. Beta-carotene attenuates the paradoxical effect of tobacco smoke on the mortality of rats after experimental myocardial infarction. J Nutr 2005; 135: 2109-13.
30. Kubes P, McCafferty DM. Nitric oxide and intestinal inflammation. Am J Med 2000; 109: 150-8.
31. Cantré D, Schuett H, Hildebrandt A, et al. Nitric oxide reduces organ injury and enhances regeneration of reducedsize livers by increasing hepatic arterial flow. Br J Surg 2008; 95: 785-92.
32. Schoen JM, Wang HH, Minuk GY, Lautt WW. Shear stress-induced nitric oxide release triggers the liver regeneration cascade. Nitric Oxide 2001; 5: 453-64.
33. Li J, Billiar TR. The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia reperfusion. Am J Physiol 1999; 276: 1069-73.
34. Hunt NC, Goldin RD. Nitric oxide production by monocytes in alcoholic liver disease. J Hepatol 1992; 14: 146-50.
35. Werner J, Fernandez-Del Castillo C, Rivera JA, et al. On the protective mechanisms of nitric oxide in acute pancreatitis. Gut 1998; 43: 401-7.
36. Gitenay D, Lyan B, Rambeau M, et al. Comparison of lycopene and tomato effects on biomarkers of oxidative stress in vitamin E deficient rats. Eur J Nutr 2007; 46: 468-75.
37. Ben-Dor A, Steiner M, Gheber L, et al. Carotenoids activate the antioxidant response element transcription system. Mol Cancer Ther 2005; 4: 177-86.
38. Nara E, Hayashi H, Kotake M, et al. Acyclic carotenoids and their oxidation mixtures inhibit the growth of HL-60 human promyelocytic leukemia cells. Nutr Cancer 2001; 39: 273-83.
39. Basu A, Imrhan V. Tomatoes versus lycopene in oxidative stress and carcinogenesis: conclusions from clinical trials. Eur J Clin Nutr 2007; 61: 295- 303.

APA	Ayan F, Aytac E, SAYGILI S, GENÇ H, Karaca C, BAYRAK İ, Uzun H, SALİHOĞLU Z, Erdamar S, Sozer V, ALTUĞ T, SEYMEN P, SEYMEN H (2011). Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. , 408 - 413.
Chicago	Ayan Fadıl,Aytac Erman,SAYGILI Seha,GENÇ Habibe,Karaca Cetin,BAYRAK İbrahim,Uzun Hafize,SALİHOĞLU Ziya,Erdamar Sibel,Sozer Volkan,ALTUĞ Tuncay,SEYMEN Pınar,SEYMEN HAKKI OKTAY Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. (2011): 408 - 413.
MLA	Ayan Fadıl,Aytac Erman,SAYGILI Seha,GENÇ Habibe,Karaca Cetin,BAYRAK İbrahim,Uzun Hafize,SALİHOĞLU Ziya,Erdamar Sibel,Sozer Volkan,ALTUĞ Tuncay,SEYMEN Pınar,SEYMEN HAKKI OKTAY Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. , 2011, ss.408 - 413.
AMA	Ayan F,Aytac E,SAYGILI S,GENÇ H,Karaca C,BAYRAK İ,Uzun H,SALİHOĞLU Z,Erdamar S,Sozer V,ALTUĞ T,SEYMEN P,SEYMEN H Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. . 2011; 408 - 413.
Vancouver	Ayan F,Aytac E,SAYGILI S,GENÇ H,Karaca C,BAYRAK İ,Uzun H,SALİHOĞLU Z,Erdamar S,Sozer V,ALTUĞ T,SEYMEN P,SEYMEN H Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. . 2011; 408 - 413.
IEEE	Ayan F,Aytac E,SAYGILI S,GENÇ H,Karaca C,BAYRAK İ,Uzun H,SALİHOĞLU Z,Erdamar S,Sozer V,ALTUĞ T,SEYMEN P,SEYMEN H "Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats." , ss.408 - 413, 2011.
ISNAD	Ayan, Fadıl vd. "Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats". (2011), 408-413.

APA	Ayan F, Aytac E, SAYGILI S, GENÇ H, Karaca C, BAYRAK İ, Uzun H, SALİHOĞLU Z, Erdamar S, Sozer V, ALTUĞ T, SEYMEN P, SEYMEN H (2011). Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. Turkish Journal of Gastroenterology, 22(4), 408 - 413.
Chicago	Ayan Fadıl,Aytac Erman,SAYGILI Seha,GENÇ Habibe,Karaca Cetin,BAYRAK İbrahim,Uzun Hafize,SALİHOĞLU Ziya,Erdamar Sibel,Sozer Volkan,ALTUĞ Tuncay,SEYMEN Pınar,SEYMEN HAKKI OKTAY Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. Turkish Journal of Gastroenterology 22, no.4 (2011): 408 - 413.
MLA	Ayan Fadıl,Aytac Erman,SAYGILI Seha,GENÇ Habibe,Karaca Cetin,BAYRAK İbrahim,Uzun Hafize,SALİHOĞLU Ziya,Erdamar Sibel,Sozer Volkan,ALTUĞ Tuncay,SEYMEN Pınar,SEYMEN HAKKI OKTAY Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. Turkish Journal of Gastroenterology, vol.22, no.4, 2011, ss.408 - 413.
AMA	Ayan F,Aytac E,SAYGILI S,GENÇ H,Karaca C,BAYRAK İ,Uzun H,SALİHOĞLU Z,Erdamar S,Sozer V,ALTUĞ T,SEYMEN P,SEYMEN H Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. Turkish Journal of Gastroenterology. 2011; 22(4): 408 - 413.
Vancouver	Ayan F,Aytac E,SAYGILI S,GENÇ H,Karaca C,BAYRAK İ,Uzun H,SALİHOĞLU Z,Erdamar S,Sozer V,ALTUĞ T,SEYMEN P,SEYMEN H Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats. Turkish Journal of Gastroenterology. 2011; 22(4): 408 - 413.
IEEE	Ayan F,Aytac E,SAYGILI S,GENÇ H,Karaca C,BAYRAK İ,Uzun H,SALİHOĞLU Z,Erdamar S,Sozer V,ALTUĞ T,SEYMEN P,SEYMEN H "Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats." Turkish Journal of Gastroenterology, 22, ss.408 - 413, 2011.
ISNAD	Ayan, Fadıl vd. "Effects of lycopene on oxidative stress and remnant liver histology after partial hepatectomy in rats". Turkish Journal of Gastroenterology 22/4 (2011), 408-413.