Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion

Baltalarlı, Ahmet; SAÇKAN, Kadir Gökhan; ÖNEM, Gökhan; SAÇAR, Mustafa; AYBEK, Hülya; ADALI, Fahri; KOCAMAZ, ERDOĞAN

Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion

Gökhan ÖNEM, (Pamukkale Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Denizli, Türkiye)

Mustafa SAÇAR, (Pamukkale Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Denizli, Türkiye)

Hülya AYBEK, (Pamukkale Üniversitesi, Tıp Fakültesi, Biyokimya Bölümü, Denizli, Türkiye)

Erdoğan KOCAMAZ, (Pamukkale Üniversitesi, Tıp Fakültesi, Histoloji Embriyoloji Anabilim Dalı, Denizli, Türkiye)

Fahri ADALI, (Balıkesir Devlet Hastanesi, Kalp Damar Cerrahisi Anabilim Dalı, Balıkesir, Türkiye)

Kadir Gökhan SAÇKAN, (Pamukkale Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Denizli, Türkiye)

Ahmet BALTALARLI (Pamukkale Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Denizli, Türkiye)

Türk Göğüs Kalp Damar Cerrahisi Dergisi

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Yıl: 2012 Cilt: 20 Sayı: 3 Sayfa Aralığı: 577 - 583 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion

Öz:

Amaç: Bu çalışmada, abdominal aort cerrahisinde iskemi/ reperfüzyon (İ/R) sonrası uzak organ hasarı olarak akciğer üzerine silostazol, levosimendan ve bu ilaçların kombinasyonunun etkileri araştırıldı. Çalışma planı: Çalışma ortalama 219±26 g ağırlığında 35 erkek Wistar albino türü sıçan ile gerçekleştirildi. Sıçanlar her bir grupta yedi sıçan olacak şekilde randomize olarak beş gruba ayrıldı. Sıçanlara silostazol, levosimendan ve bu iki ilacın kombinasyonu ile tedavi uygulandıktan sonra, infrarenal aortik oklüzyon ile alt ekstremitelere 120 dakika iskemi ve sonrasında 60 dakika reperfüzyon uygulandı. Sıçanlar derin anestezi altında sakrifiye edildi ve akciğer dokuları çıkarıldı. Akciğer dokularında malondialdehid (MDA) düzeyleri, süperoksit dismutaz (SOD) aktiviteleri ve glutatyon (GSH) düzeyleri ölçüldü. Doku örnekleri ayrıca ışık mikroskobu ile histopatolojik olarak incelendi. Bulgular: Çalışmada İ/R’nin akciğer dokusunda MDA düzeylerini yükseltirken, SOD aktivitesini ve GSH düzeylerini azalttığı belirlendi (p<0.05). Silostazol, levosimendan ve bu ilaçların kombinasyonu ile MDA düzeyleri, SOD aktiviteleri, GSH düzeyleri ve akciğer hasarı skorunda iyileşme gözlendi (p<0.05). Bu ilaçların tek veya kombine kullanılması arasında anlamlı farklılık yoktu (p>0.05). Sonuç: Bu bulgular ışığında, vasküler cerrahi sırasında ekstremite iskemisi öncesi silostazol ve levosimendan kullanımı akciğer dokusunu İ/R hasarından korumakta faydalı olabilir. Bununla birlikte, bu iki ilacın kombine kullanımı, İ/R hasarına karşı koruyuculuğu artırmamaktadır.

Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi Cerrahi

Alt ekstremite iskemi reperfüzyonuna bağlı akciğer hasarında silostazol ve levosimendanın koruyucu etkisi

Öz:

Background: In this study, we aimed to investigate the effects of cilostazol and levosimendan, and the combination of these agents on the lung remote organ damage after ischemia/reperfusion (I/R) following abdominal aortic surgery. Methods: The experiments were performed on 35 male Wistar albino rats weighing mean 219±26 g. The rats were randomly assigned into five groups, including each of seven rats. Rats were pretreated with cilostazol and levosimendan, alone or in combination, and then lower extremities were subjected to I/R induced by a infrarenal aortic occlusion for duration of 120 minutes, followed by a-60 minute- reperfusion. The rats were sacrificed under deep anesthesia and the lung tissues were removed. Malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, and glutathione (GSH) levels were measured in the lung tissues. The tissue samples were further examined histopathologically under light microscopy. Results: It was found that I/R elevated MDA levels accompanied by a reduction in SOD activities and GSH levels (p<0.05). Cilostazol and levosimendan, and their combination restored MDA levels, SOD activity, GSH levels and lung injury scores (p<0.05). There was no significant difference among individual or combined treatment of these agents (p>0.05). Conclusion: In light of these findings, cilostazol and levosimendan may be useful for protecting the lung tissue from I/R injury, before limb ischemia in vascular surgery. However, the combined use of these agents does not further increase the protection from I/R injury.

Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÖNEM G, SAÇAR M, AYBEK H, KOCAMAZ E, ADALI F, SAÇKAN K, Baltalarlı A (2012). Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. , 577 - 583.
Chicago	ÖNEM Gökhan,SAÇAR Mustafa,AYBEK Hülya,KOCAMAZ ERDOĞAN,ADALI Fahri,SAÇKAN Kadir Gökhan,Baltalarlı Ahmet Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. (2012): 577 - 583.
MLA	ÖNEM Gökhan,SAÇAR Mustafa,AYBEK Hülya,KOCAMAZ ERDOĞAN,ADALI Fahri,SAÇKAN Kadir Gökhan,Baltalarlı Ahmet Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. , 2012, ss.577 - 583.
AMA	ÖNEM G,SAÇAR M,AYBEK H,KOCAMAZ E,ADALI F,SAÇKAN K,Baltalarlı A Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. . 2012; 577 - 583.
Vancouver	ÖNEM G,SAÇAR M,AYBEK H,KOCAMAZ E,ADALI F,SAÇKAN K,Baltalarlı A Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. . 2012; 577 - 583.
IEEE	ÖNEM G,SAÇAR M,AYBEK H,KOCAMAZ E,ADALI F,SAÇKAN K,Baltalarlı A "Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion." , ss.577 - 583, 2012.
ISNAD	ÖNEM, Gökhan vd. "Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion". (2012), 577-583.

APA	ÖNEM G, SAÇAR M, AYBEK H, KOCAMAZ E, ADALI F, SAÇKAN K, Baltalarlı A (2012). Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. Türk Göğüs Kalp Damar Cerrahisi Dergisi, 20(3), 577 - 583.
Chicago	ÖNEM Gökhan,SAÇAR Mustafa,AYBEK Hülya,KOCAMAZ ERDOĞAN,ADALI Fahri,SAÇKAN Kadir Gökhan,Baltalarlı Ahmet Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. Türk Göğüs Kalp Damar Cerrahisi Dergisi 20, no.3 (2012): 577 - 583.
MLA	ÖNEM Gökhan,SAÇAR Mustafa,AYBEK Hülya,KOCAMAZ ERDOĞAN,ADALI Fahri,SAÇKAN Kadir Gökhan,Baltalarlı Ahmet Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. Türk Göğüs Kalp Damar Cerrahisi Dergisi, vol.20, no.3, 2012, ss.577 - 583.
AMA	ÖNEM G,SAÇAR M,AYBEK H,KOCAMAZ E,ADALI F,SAÇKAN K,Baltalarlı A Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2012; 20(3): 577 - 583.
Vancouver	ÖNEM G,SAÇAR M,AYBEK H,KOCAMAZ E,ADALI F,SAÇKAN K,Baltalarlı A Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2012; 20(3): 577 - 583.
IEEE	ÖNEM G,SAÇAR M,AYBEK H,KOCAMAZ E,ADALI F,SAÇKAN K,Baltalarlı A "Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion." Türk Göğüs Kalp Damar Cerrahisi Dergisi, 20, ss.577 - 583, 2012.
ISNAD	ÖNEM, Gökhan vd. "Protective effects of cilostazol and levosimendan on lung injury induced by lower limb ischemia-reperfusion". Türk Göğüs Kalp Damar Cerrahisi Dergisi 20/3 (2012), 577-583.