Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique

GÜNAYDIN, Serdar; ONUR, MEHMET ALİ; NİYAZ, Mehmet; GÜRPINAR, Özer Aylin

Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique

Mehmet NİYAZ, (Kırıkkale Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Kırıkkale, Türkiye)

Özer Aylin GÜRPINAR, (Kırıkkale Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Kırıkkale, Türkiye)

Serdar GÜNAYDIN, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Mehmet Ali ONUR (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Turkish Journal of Biology

8 0

Yıl: 2012 Cilt: 36 Sayı: 6 Sayfa Aralığı: 658 - 664 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique

Öz:

In this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD 13 and CD29 markers. The results showed that these cultured cells were positive for CD 13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class I, CD45, CD106, and MHC Class II for characterization of mesenchymal stem cells. The results of flow cytometry analysis showed that these cells were mesenchymal stem cells. Half of the cells were cryopreserved at all passages for future applications. It is thought that these mesenchymal stem cells can be used in therapy of cardiovascular diseases as an alternative technique in the near future.

Anahtar Kelime: cryopreservation immunofluorescence rats staining isolation adipose tissue

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	NİYAZ M, GÜRPINAR Ö, GÜNAYDIN S, ONUR M (2012). Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. , 658 - 664.
Chicago	NİYAZ Mehmet,GÜRPINAR Özer Aylin,GÜNAYDIN Serdar,ONUR MEHMET ALİ Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. (2012): 658 - 664.
MLA	NİYAZ Mehmet,GÜRPINAR Özer Aylin,GÜNAYDIN Serdar,ONUR MEHMET ALİ Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. , 2012, ss.658 - 664.
AMA	NİYAZ M,GÜRPINAR Ö,GÜNAYDIN S,ONUR M Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. . 2012; 658 - 664.
Vancouver	NİYAZ M,GÜRPINAR Ö,GÜNAYDIN S,ONUR M Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. . 2012; 658 - 664.
IEEE	NİYAZ M,GÜRPINAR Ö,GÜNAYDIN S,ONUR M "Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique." , ss.658 - 664, 2012.
ISNAD	NİYAZ, Mehmet vd. "Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique". (2012), 658-664.

APA	NİYAZ M, GÜRPINAR Ö, GÜNAYDIN S, ONUR M (2012). Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. Turkish Journal of Biology, 36(6), 658 - 664.
Chicago	NİYAZ Mehmet,GÜRPINAR Özer Aylin,GÜNAYDIN Serdar,ONUR MEHMET ALİ Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. Turkish Journal of Biology 36, no.6 (2012): 658 - 664.
MLA	NİYAZ Mehmet,GÜRPINAR Özer Aylin,GÜNAYDIN Serdar,ONUR MEHMET ALİ Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. Turkish Journal of Biology, vol.36, no.6, 2012, ss.658 - 664.
AMA	NİYAZ M,GÜRPINAR Ö,GÜNAYDIN S,ONUR M Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. Turkish Journal of Biology. 2012; 36(6): 658 - 664.
Vancouver	NİYAZ M,GÜRPINAR Ö,GÜNAYDIN S,ONUR M Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique. Turkish Journal of Biology. 2012; 36(6): 658 - 664.
IEEE	NİYAZ M,GÜRPINAR Ö,GÜNAYDIN S,ONUR M "Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique." Turkish Journal of Biology, 36, ss.658 - 664, 2012.
ISNAD	NİYAZ, Mehmet vd. "Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: A simple technique". Turkish Journal of Biology 36/6 (2012), 658-664.