Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress

AKONDI, Raju B.; Challa, Siva Reddy; AKULA, Annapurna

Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress

Raju B. AKONDI, (College of Pharmaceutical Sciences, Division of Pharmacology, Andhra University, Visakhapatnam, India)

Annapurna AKULA, (College of Pharmaceutical Sciences, Division of Pharmacology, Andhra University, Visakhapatnam, India)

Siva Reddy CHALLA (College of Pharmaceutical Sciences, Division of Pharmacology, Andhra University, Visakhapatnam, India)

European Journal of General Medicine

2 0

Yıl: 2011 Cilt: 8 Sayı: 1 Sayfa Aralığı: 57 - 64 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress

Öz:

Amaç: Bu çalışmanın amacı ratlarda gentamisinin neden olduğu testiküler oksidatif stress üzerine bioflavonoid bileşiklerinden olan rutin ve naringinin koruyucu etkisini araştırmaktır. Metod: Toplam 42 adet wistar albino ratlar 6’lı gruplar halinde 7 gruba ayrıldı. Gentamisin 5 mg/kg 1. günden 10. güne kadar intraperitoneal olarak uygulandı. Rutin ve naringin 5 mg/kg ve 10 mg/kg dozlarında gentamisin ile birlikte verilmek üzere toplam 35 gün verildi. Kontrol grubu olarak 35 gün boyunca gentamisin, 0.1% sod CMC ve su alan ratlar çalışmada kullanıldı. Çalışmanın 36. günü tüm ratlara orşiektomi yapılarak sperm parametreleri, histolojik değişiklikler ve biokimyasal parametreler değerlendirildi.Bulgular: 5 mg/kg ve 10 mg/kg rutin ve naringin ile tedavi edilen ratlarda (grup 4,5,6,7) kontrol grubuna göre doz bağımlı olarak anlamlı ölçüde serum MDA seviyesinde azalma ve SOD ve katalaz gibi antioksidan enzim düzeylerinde artış tespit edildi. Rutin ve nargirin tedavisi altında sperm sayısı, motilitesi ve viabilitesi korunmuş olarak bulundu. Grup 2’den elde edilen hücre örneklerinde histolojik olarak germinal hücre hasarı mevcuttu. İlaçla tedavi edilen gruplarda testiküler dokuda iyileşme saptandı. Naringin ile karşılaştırıldında rutin tüm parameterler üzerine daha iyi etkilere sahipti. Her iki bioflavonoid gentamisin nedenli testiküler stresste etkili bulundu. Sonuç: Rutin ve naringin tedavisi ratlarda gentamisin nedenli oksidatif stressi zaltmada koruyucu ve etkin bir tedavi sağlamıştır.

Anahtar Kelime:

Konular: Cerrahi

Gentamisinin meydana getirdiği testiküler oksidatif streste rutin ve naringinin koruyucu etkisi

Öz:

Aim: The aim of this study was to investigate the protective effects of bioflavonoid compounds i.e. rutin and naringin on gentamycin induced testicular oxidative stress in rats. Method: A total of 42 male wistar albino rats were divided in to 7 groups, each contains 6 rats. Gentamycin, 5 mg/kg, I.P was administered to the rats from day 1 to day 10. Rutin and naringin were given in different doses (5mg/kg, 10/kg) for a period of 35 days from day 1 to day 35 to the gentamycin treated animals. A sham control group animals with water, control group animals with gentamycin and vehicle control group animals with 0.1% sod CMC were also maintained until completion of 35 days. On 36th day bilateral orchiectomies were performed for all the animals. The testicular tissue was evaluated for various sperm parameters, biochemical estimations and histological changes. Result: Animals treated with 5mg/kg and 10mg/kg of rutin and naringin (Groups 4, 5, 6, 7) have shown significant and dose dependent reduction in MDA levels and increase in levels of antioxidant enzymes, SOD and Catalase when compared to control group animals (Group 2). Sperm count, motility, viability were also protected and normalized with rutin and naringin. Specimens from group 2 had a histological injury with disordered germinal cells. Drug treated groups shown improved testicular architecture. Rutin was more effective in all the parameters when compared to Naringin. Both the bioflavonoids were effective in reducing the gentamycin induced testicular oxidative stress. Conclusion: Rutin and Naringin pretreatment have shown protective and beneficial effect on gentamycin induced oxidative stress in rats.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AKONDI R, AKULA A, Challa S (2011). Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. , 57 - 64.
Chicago	AKONDI Raju B.,AKULA Annapurna,Challa Siva Reddy Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. (2011): 57 - 64.
MLA	AKONDI Raju B.,AKULA Annapurna,Challa Siva Reddy Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. , 2011, ss.57 - 64.
AMA	AKONDI R,AKULA A,Challa S Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. . 2011; 57 - 64.
Vancouver	AKONDI R,AKULA A,Challa S Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. . 2011; 57 - 64.
IEEE	AKONDI R,AKULA A,Challa S "Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress." , ss.57 - 64, 2011.
ISNAD	AKONDI, Raju B. vd. "Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress". (2011), 57-64.

APA	AKONDI R, AKULA A, Challa S (2011). Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. European Journal of General Medicine, 8(1), 57 - 64.
Chicago	AKONDI Raju B.,AKULA Annapurna,Challa Siva Reddy Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. European Journal of General Medicine 8, no.1 (2011): 57 - 64.
MLA	AKONDI Raju B.,AKULA Annapurna,Challa Siva Reddy Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. European Journal of General Medicine, vol.8, no.1, 2011, ss.57 - 64.
AMA	AKONDI R,AKULA A,Challa S Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. European Journal of General Medicine. 2011; 8(1): 57 - 64.
Vancouver	AKONDI R,AKULA A,Challa S Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress. European Journal of General Medicine. 2011; 8(1): 57 - 64.
IEEE	AKONDI R,AKULA A,Challa S "Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress." European Journal of General Medicine, 8, ss.57 - 64, 2011.
ISNAD	AKONDI, Raju B. vd. "Protective effects of rutin and naringin on gentamycin induced testicular oxidative stress". European Journal of General Medicine 8/1 (2011), 57-64.