Yıl: 2013 Cilt: 37 Sayı: 1 Sayfa Aralığı: 153 - 159 Metin Dili: İngilizce

Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors

Öz:
The goal of this work was to study the stimulating effects of mannan and pectin on the biosynthesis of hypericins in in vitro seedlings of Hypericum adenotrichum Spach. Seedlings of H. adenotrichum were grown on a modified MS medium containing mannan (10, 50, and 100 mg/L) or pectin (10, 50, and 100 mg/L) for 15 and 30 days. Seedlings of H. adenotrichum were extracted with methanol. The methanol extracts of the seedlings were analysed by HPLC to investigate the changes in hypericin and pseudohypericin levels. The best results were obtained by exposure to 50 mg/L mannan or pectin for 15 days. Mannan stimulated pseudohypericin production up to 2.8-fold and hypericin production up to 1.7-fold. Pectin stimulated pseudohypericin production up to 4.8-fold and hypericin production up to 2.7-fold. The increases in hypericins in response to mannan and pectin demonstrate that these elicitors can be evaluated for production of secondary metabolites in Hypericum species.
Anahtar Kelime: Hypericum elicitors mannans biosynthesis pectins Hypericum adenotrichum

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA YAMANER Ö, ERDAĞ B, GÖKBULUT C (2013). Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors. Turkish Journal of Botany, 37(1), 153 - 159.
Chicago YAMANER Ömer,ERDAĞ BENGİ,GÖKBULUT CENGİZ Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors. Turkish Journal of Botany 37, no.1 (2013): 153 - 159.
MLA YAMANER Ömer,ERDAĞ BENGİ,GÖKBULUT CENGİZ Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors. Turkish Journal of Botany, vol.37, no.1, 2013, ss.153 - 159.
AMA YAMANER Ö,ERDAĞ B,GÖKBULUT C Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors. Turkish Journal of Botany. 2013; 37(1): 153 - 159.
Vancouver YAMANER Ö,ERDAĞ B,GÖKBULUT C Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors. Turkish Journal of Botany. 2013; 37(1): 153 - 159.
IEEE YAMANER Ö,ERDAĞ B,GÖKBULUT C "Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors." Turkish Journal of Botany, 37, ss.153 - 159, 2013.
ISNAD YAMANER, Ömer vd. "Stimulation of the production of hypericins in in vitro seedlings of Hypericum adenotrichum by some biotic elicitors". Turkish Journal of Botany 37/1 (2013), 153-159.