Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey

CARLI, KAMİL TAYFUN; Eyigor, Aysegul; Coven, Fethiye; KAHYA DEMİRBİLEK, SERPİL; TEMELLİ, Seran

Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey

SERPİL KAHYA DEMİRBİLEK, (Uludağ Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Bursa)

Fethiye ÇÖVEN, (Bornova Veteriner Kontrol ve Araştırma Enstitüsü, İzmir, Türkiye)

Seran TEMELLİ, (Uludağ Üniversitesi, Veteriner Fakültesi, Gıda Hijyeni ve Teknoloji Anabilim Dalı, Bursa, Türkiye)

Ayşegül EYİGÖR, (Uludağ Üniversitesi, Veteriner Fakültesi, Gıda Hijyeni ve Teknoloji Anabilim Dalı, Bursa, Türkiye)

Kamil Tayfun CARLI (Uludağ Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Bursa)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

10 4

Yıl: 2013 Cilt: 60 Sayı: 1 Sayfa Aralığı: 27 - 31 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey

Öz:

Bu çalışmada H120 suşu ile aşılanmış 2 broyler ve 8 damızlık sürüde infeksiyöz bronşit şüpheli problemlere rastlanmış 2 sürüye ait 60 broyler tavuktan (30 svap/sürü) ve 8 sürüye ait 160 damızlık tavuktan (20 svap/sürü) özellikle solunum bulguları ile nefrit lezyonları gösterenlerden toplam 220 trakeal svap infeksiyöz bronşit virusu (IBV)’nu reverse transcriptase polymerase chain reaction (RT-PCR) ile belirlemek ve IBV’yi izole etmek amacı ile alındı. IBV’nin trakeal svap örneklerinden direkt olarak RT-PCR ile deteksiyonu embryolu yumurtaların allantoik sıvılarından IBV izolasyonu ve bu örneklerden RT-PCR yapılması ile doğrulandı. Basic Local Alignment Search Tool (BLAST) sonuçları 8 Türk izolatının EU780077 (IS/1494/06 IBV suşu) ile %99 benzerlik gösterdiğini ortaya koydu. Broyler sürüden TR_IS1494_06_B12011 ve damızlık sürüden TR_1494_06_7201 olarak seçilen temsili iki izolatın dizilerinin filogenetik ve sıralama analizleri de IS/1494/06 IBV genotipine yakınlığı doğruladı. Sonuç olarak, Türkiye’de IBV H120 suşu ile aşılamaya rağmen IB’den korunamayan tavuklardan ilk defa izole edilmesi ve IS/1494/06 olarak genotiplendirilmesi bu IBV’nin Orta Doğu ülkelerinden taşınma olasılığını akla getirmektedir.

Anahtar Kelime: epidemiyoloji hastalık bulaşması nükleotit dizileri teşhis aşılama (hayvan) hastalık önleme klinik görünüm kümes hayvanları nefrit hastalık tetkiki bronşit epidemiyolojik araştırmalar hastalık yaygınlığı Türkiye filogeni etlik piliç nefes borusu genotip filogenetik yumurta tavuğu viral hastalıklar tavuk

Konular: Veterinerlik

Türkiye’de damızlık ve broyler sürülerde IS/1494/06 genotipi-ilişkili infeksiyöz bronşit virusunun varlığı

Öz:

In this study, infectious bronchitis (IB)-suspect problems were observed in 2 broiler and 8 breeder chicken flocks vaccinated with H120 strain, and a total of 220 tracheal swabs were taken from 60 broiler chickens of 2 flocks (30 swabs/flock), and 160 breeder chickens of 8 flocks (20 swabs/flock) to detect infectious bronchitis virus (IBV) by a reverse transcriptase polymerase chain reaction (RT-PCR) and to isolate IBV from chickens showing respiratory sings and nephritis lesions. Detection of 8 Turkish IBVs from the tracheal swab samples directly by RT-PCR was confirmed by both IBV isolation from allantoic fluids of embryonated eggs and by the same RT-PCRs from these samples. Basic Local Alignment Search Tool (BLAST) results revealed that all 8 sequences from our Turkish IBV isolates were related to that of EU780077 (IS/1494/06 IBV strain) with a 99% identity. Phylogenetic and alignment analyses of the sequences of two representative isolates as TR_IS1494_06_B12011 from one broiler flock and TR_1494_06_7201 from one breeder flock also confirmed this close relation to IS/1494/06 IBV genotype. As a result, Turkish IBVs have been isolated for the first time from chickens, which were not protected from IB despite IBV H120 strain vaccination, and genotyped as IS/1494/06 in Turkey suggesting the transfer of this IBV from the countries of Middle East.

Anahtar Kelime: disease surveys bronchitis epidemiological surveys disease prevalence Turkey phylogeny broilers trachea genotypes phylogenetics hens viral diseases fowls epidemiology disease transmission nucleotide sequences diagnosis vaccination disease prevention clinical aspects poultry nephritis

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Bochkov YA, Batchenko GV, Shcherbakova LO, Borisov AV, Drygin VV (2006): Molecular epizootiology of avian infectious bronchitis in Russia. Avian Pathol, 35, 379–393.
2. Boursnell MEG, Brown TDK, Foulds IJ, Green PF, Tomley FM, Binns MM (1987): Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol, 68, 57–77.
3. Cavanagh D, Davis PJ, Pappin DJ, Binns MM, Boursnell ME, Brown TD (1986): Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41. Virus Res, 4, 133–143.
4. Cavanagh D, Davis PJ, Cook J (1992): Infectious bronchitis virus: evidence for recombination within the Massachusetts serotype. Avian Pathology, 21, 401–408.
5. Cavanagh D (1997): Nidovirales: A new order comprising Coronoviridae and Arteriviridae. Arch Virol, 142,629– 633.
6. Cook JKA, Jackwood M, Jones RC (2012): The long view: 40 years of infectious bronchitis research. Avian Pathol, 41, 239-250.
7. De Wit JJ, Cook JKA, van der Heijden HMJF (2011): Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol, 40, 223-235.
8. Gelb J, Weisman Y, Ladman BS, Meir R (2005): S1 gene characteristics and efficacy of vaccination against infectious bronchitis virus field isolates from the United States and Israel (1996 to 2000). Avian Pathol, 34, 194– 203.
9. Ignjatovic J, Sapats S (2000): Avian infectious bronchitis virus. The OIE Scientific and Technical Review, 19, 493– 508.
10. Kingham BF, Keeler CL Jr, Nix WA, Ladman BS, Gelb J Jr (2000): Identification of avian infectious bronchitis virus by direct automated cycle sequencing of the S-1 gene. Avian Dis, 44,325–335.
11. Kottier SA, Cavanagh D, Britton P (1995): Experimental evidence of recombination in coronavirus infectious bronchitis virus. Virology, 213, 569–580.
12. Lee CW, Jackwood MW (2000): Evidence of genetic diversity generated by recombination among avian coronovirus IBV. Arch Virol, 145, 2135–2148.
13. Mahmood ZH, Sleman RR Uthman AU (2011): Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet Microbiol, 150, 21–27.
14. OIE Terrestrial Manual 2008. Chapter 2.3.2. (2008): Avian Infectious Bronchitis. pp. 443–455.
15. Pohuang T, Chansiripornchai N, Tawatsin A, Sasipreeyajan J (2009): Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. J Vet Sci 10, 219–223.

APA	KAHYA DEMİRBİLEK S, Coven F, TEMELLİ S, Eyigor A, CARLI K (2013). Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. , 27 - 31.
Chicago	KAHYA DEMİRBİLEK SERPİL,Coven Fethiye,TEMELLİ Seran,Eyigor Aysegul,CARLI KAMİL TAYFUN Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. (2013): 27 - 31.
MLA	KAHYA DEMİRBİLEK SERPİL,Coven Fethiye,TEMELLİ Seran,Eyigor Aysegul,CARLI KAMİL TAYFUN Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. , 2013, ss.27 - 31.
AMA	KAHYA DEMİRBİLEK S,Coven F,TEMELLİ S,Eyigor A,CARLI K Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. . 2013; 27 - 31.
Vancouver	KAHYA DEMİRBİLEK S,Coven F,TEMELLİ S,Eyigor A,CARLI K Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. . 2013; 27 - 31.
IEEE	KAHYA DEMİRBİLEK S,Coven F,TEMELLİ S,Eyigor A,CARLI K "Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey." , ss.27 - 31, 2013.
ISNAD	KAHYA DEMİRBİLEK, SERPİL vd. "Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey". (2013), 27-31.

APA	KAHYA DEMİRBİLEK S, Coven F, TEMELLİ S, Eyigor A, CARLI K (2013). Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 60(1), 27 - 31.
Chicago	KAHYA DEMİRBİLEK SERPİL,Coven Fethiye,TEMELLİ Seran,Eyigor Aysegul,CARLI KAMİL TAYFUN Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi 60, no.1 (2013): 27 - 31.
MLA	KAHYA DEMİRBİLEK SERPİL,Coven Fethiye,TEMELLİ Seran,Eyigor Aysegul,CARLI KAMİL TAYFUN Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.60, no.1, 2013, ss.27 - 31.
AMA	KAHYA DEMİRBİLEK S,Coven F,TEMELLİ S,Eyigor A,CARLI K Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2013; 60(1): 27 - 31.
Vancouver	KAHYA DEMİRBİLEK S,Coven F,TEMELLİ S,Eyigor A,CARLI K Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2013; 60(1): 27 - 31.
IEEE	KAHYA DEMİRBİLEK S,Coven F,TEMELLİ S,Eyigor A,CARLI K "Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 60, ss.27 - 31, 2013.
ISNAD	KAHYA DEMİRBİLEK, SERPİL vd. "Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 60/1 (2013), 27-31.