Yıl: 2013 Cilt: 37 Sayı: 1 Sayfa Aralığı: 106 - 113 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins

Öz:
An efficient in vitro protocol for propagation of Stevia rebaudiana Bertoni is described. Multiple shoots were induced in vitro from shoot tip and nodal segments on Murashige and Skoog medium containing 6-benzylaminopurine, zeatin, or thidiazuron alone and in combination with naphthalene acetic acid or indole-3-acetic acid. A high frequency of shoot induction as well as maximum number of shoots per shoot tip explant was observed on Murashige and Skoog medium supplemented with 6-benzylaminopurine (1.0 mg L 1) alone and combined with indole-3-acetic acid (0.1 mg L 1 ). For root induction, in vitro shoots were transferred to rooting media containing naphthalene acetic acid, indole-3-acetic acid, or indole-3-butyric acid. The highest rooting frequency and the highest number of roots was observed in half-strength Murashige and Skoog medium supplemented with 0.1 mg L 1 indole-3-butyric acid. The rooted in vitro plants were successfully acclimatized in a growth chamber and transferred to the field. Leaf extracts of plants propagated in vitro and adapted to field conditions are characterized by high levels of water-soluble antioxidant capacity (expressed as equivalents of ascorbic acid), phenols, and flavonoids, and therefore by high total antioxidant potential, expressed as DPPH radical scavenging activity
Anahtar Kelime: acclimatization shoots medicinal plants node micropropagation Stevia rebaudiana

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1.Duke J. Stevia rebaudiana. In: Duke J. ed. CRC Handbook of Alternative Cash Crops. CRC Press; 1993: pp. 422–424.
  • 2.Goettemoeller J, Ching A. Seed germination in Stevia rebaudiana. In: Janick J. ed. Perspectives on New Crops and New Uses. ASHS Press; 1999: pp. 510–511.
  • 3.Bhosle S. Commercial cultivation of Stevia rebaudiana. Agrobios Newslett 3: 43–45, 2004.
  • 4.Gujral S. Stevia: 0% calorie, 100% sweet, 100% nature. Sci Tech Entrepreneur 12: 1–7, 2004
  • 5.Kim IS, Yang M, Lee OH et al. The antioxidant activity and the bioactive compound content of Stevia rebaudiana water extracts. LWT Food Sci Tech 44: 1328–1332, 2011.
  • 6.Komissarenko NF, Derkach AI, Kovalyov IP at al. Diterpene glycosides and phenylpropanoids of Stevia rebaudiana Bertoni. Rast Research 1: 53–64, 1994.
  • 7.Hollman PCH, Hertog MGL, Katan MB. Analysis and health effects of flavonoids. Food Chem 57: 43–46, 1996.
  • 8.Siddhuraju P, Becker K. The antioxidant and free radical scavenging activities of processed cowpea ( Vigna unguiculata (L.) Walp.) seed extracts. Food Chem 101: 10–19, 2007.
  • 9.Tadhani MB, Patel VH, Subhash R. In vitro antioxidant activity of Stevia rebaudiana leaves and callus. J Food Comp Anal 20: 323–329, 2007.
  • 10.Megeji NW, Kumar JK, Singh V et al. Introducing Stevia rebaudiana, a natural zero-calorie sweetener. Curr Cell Sci 88: 801–804, 2005.
  • 11.Brandle J. Genetic control of rebaudioside A and C concentration in leaves of the sweet herb Stevia rebaudiana. Can J Plant Sci 79: 85–92, 1999.
  • 12.Kinghorn AD. Biologically active compounds from plants with reputed medicinal and sweetening properties. J Nat Prod 50: 1009–1024, 1999.
  • 13.Felippe GM, Lucas NMC. Estudo da viabilidabe dos frutos de Stevia rebaudiana. Hoehnea 1: 95–105, 1971.
  • 14.Sakaguchi M, Kan T. Japanese researches on Stevia rebaudiana. Ci Cult 34: 235–248, 1982.
  • 15.Carneiro JWP, Muniz AS, Guedes TA. Greenhouse bedding plant production of Stevia rebaudiana (Bert) Bertoni. Can J Plant Sci 77: 473–474, 1997.
  • 16.Sivaram L, Mukundan U. In vitro culture studies on Stevia rebaudiana. In Vitro Cell Dev Biol Plant 39: 520–523, 2003.
  • 17.Slavova Y, Nenkova D, Ivanova I. Study on the influence of the substance of benzymidazol upon Stevia rebaudiana Bertoni, cultivated in vitro. Bulg J Agric Sci 9: 225–228, 2003.
  • 18.Mitra A, Pal A. In vitro regeneration of Stevia rebaudiana (Bert) from nodal explants. J Plant Biochem Biotech 16: 59–62, 2007.
  • 19.Ahmed MB, Salahin M, Karim R et al. An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana) in Bangladesh. Am-Eu J Sci Res 2: 121–125, 2007.
  • 20.Debnath M. Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. J Med Plants Res 2: 45–51, 2008.
  • 21.Anbazhagan M, Kalpana M, Rajendran R et al. In vitro production of Stevia rebaudiana Bertoni. Emir J Food Agric 22: 216–222, 2010.
  • 22.Satpathy S, Das M. In vitro shoot multiplication in Stevia rebaudiana Bert., a medicinally important plant. Gen Appl Plant Physiol 36: 167–175, 2010.
  • 23.Shatnawi AM, Shibli RA, Abu-Romman SM et al. Clonal propagation and cryogenic storage of the medicinal plant Stevia rebaudiana. Span J Agric Res 9: 213–220, 2011.
  • 24.Cenkci S, Temel M, Kargıoğlu M et al. Propagation of endangered Thermopsis turcica Tan, Vural & Küçüködük using conventional and in vitro techniques. Turk J Biol 33: 327–333, 2009.
  • 25.Nakamura S, Tamura Y. Variation in the main glycosides of stevia. Jpn J Trop Agric 29: 109–116, 1985.
  • 26.Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plantarum 15: 473–479, 1962.
  • 27.Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269: 337–341, 1999.
  • 28.Tepe B, Sokmen M, Akpulat HA et al. Screening of the antioxidant potentials of six Salvia species from Turkey. Food Chem 95: 200–204, 2006.
  • 29.Pfeffer H, Dannel F, Römheld V. Are there connections between phenol metabolism, ascorbate metabolism and membrane integrity in leaves of boron-deficient sunflower plants? Physiol Plantarum 104: 479–485, 1998.
  • 30.Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64: 555–559, 1999.
  • 31.Rafiq M, Dahot MU, Mangrio SM et al. In vitro clonal propagation and biochemical analysis of field established Stevia rebaudiana Bertoni. Pak J Bot 39: 2467–474, 2007.
  • 32.Hossain MA, Shamim AHM, Jahan KTA et al. Micropropagation of stevia. Int J Sustain Crop Prod 3: 1–9, 2008.
  • 33.Uçar Türker A, Yücesan B, Gürel E. Adventitious shoot regeneration from stem internode explants of Verbena officinalis L., a medicinal plant. Turk J Biol 34: 297–304, 2010.
  • 34.Moncaleán P, Rodrígues A, Fernández B. In vitro response of Actinidia deliciosa explants to different BA incubation periods. Plant Cell Tissue Organ Cult 67: 257–266, 2001.
  • 35.Foyer CH, Souriau N, Perret S et al. Overexpression of glutathione reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees. Plant Physiol 109: 1047–1057, 1995.
  • 36.Benavente-García O, Castillo J, Lorente J et al. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food Chem 68: 457–462, 2000.
  • 37.Shukla S, Mehta A, Bajpai VK et al. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem Toxicol 47: 2338–2343, 2009.
  • 38.Kahkonen MP, Hopia AI, Vuorela HJ. Antioxidant activity of plant extracts containing phenolic compounds. J Agr Food Chem 47: 3954–3962, 1999
APA ZAYOVA E, STANCHEVA I, GENEVA M, PETROVA M, DIMITROVA L (2013). Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. , 106 - 113.
Chicago ZAYOVA Ely,STANCHEVA Ira,GENEVA Maria,PETROVA Maria,DIMITROVA Lyudmila Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. (2013): 106 - 113.
MLA ZAYOVA Ely,STANCHEVA Ira,GENEVA Maria,PETROVA Maria,DIMITROVA Lyudmila Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. , 2013, ss.106 - 113.
AMA ZAYOVA E,STANCHEVA I,GENEVA M,PETROVA M,DIMITROVA L Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. . 2013; 106 - 113.
Vancouver ZAYOVA E,STANCHEVA I,GENEVA M,PETROVA M,DIMITROVA L Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. . 2013; 106 - 113.
IEEE ZAYOVA E,STANCHEVA I,GENEVA M,PETROVA M,DIMITROVA L "Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins." , ss.106 - 113, 2013.
ISNAD ZAYOVA, Ely vd. "Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins". (2013), 106-113.
APA ZAYOVA E, STANCHEVA I, GENEVA M, PETROVA M, DIMITROVA L (2013). Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turkish Journal of Biology, 37(1), 106 - 113.
Chicago ZAYOVA Ely,STANCHEVA Ira,GENEVA Maria,PETROVA Maria,DIMITROVA Lyudmila Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turkish Journal of Biology 37, no.1 (2013): 106 - 113.
MLA ZAYOVA Ely,STANCHEVA Ira,GENEVA Maria,PETROVA Maria,DIMITROVA Lyudmila Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turkish Journal of Biology, vol.37, no.1, 2013, ss.106 - 113.
AMA ZAYOVA E,STANCHEVA I,GENEVA M,PETROVA M,DIMITROVA L Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turkish Journal of Biology. 2013; 37(1): 106 - 113.
Vancouver ZAYOVA E,STANCHEVA I,GENEVA M,PETROVA M,DIMITROVA L Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins. Turkish Journal of Biology. 2013; 37(1): 106 - 113.
IEEE ZAYOVA E,STANCHEVA I,GENEVA M,PETROVA M,DIMITROVA L "Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins." Turkish Journal of Biology, 37, ss.106 - 113, 2013.
ISNAD ZAYOVA, Ely vd. "Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins". Turkish Journal of Biology 37/1 (2013), 106-113.