TY  - JOUR
TI  - Antioxidant activity of in vitro propagated Stevia rebaudiana Bertoni plants of different origins
AB  - An efficient in vitro protocol for propagation of Stevia rebaudiana Bertoni is described. Multiple shoots were induced in vitro from shoot tip and nodal segments on Murashige and Skoog medium containing 6-benzylaminopurine, zeatin, or thidiazuron alone and in combination with naphthalene acetic acid or indole-3-acetic acid. A high frequency of shoot induction as well as maximum number of shoots per shoot tip explant was observed on Murashige and Skoog medium supplemented with 6-benzylaminopurine (1.0 mg L 1) alone and combined with indole-3-acetic acid (0.1 mg L 1 ). For root induction, in vitro shoots were transferred to rooting media containing naphthalene acetic acid, indole-3-acetic acid, or indole-3-butyric acid. The highest rooting frequency and the highest number of roots was observed in half-strength Murashige and Skoog medium supplemented with 0.1 mg L 1 indole-3-butyric acid. The rooted in vitro plants were successfully acclimatized in a growth chamber and transferred to the field. Leaf extracts of plants propagated in vitro and adapted to field conditions are characterized by high levels of water-soluble antioxidant capacity (expressed as equivalents of ascorbic acid), phenols, and flavonoids, and therefore by high total antioxidant potential, expressed as DPPH radical scavenging activity
AU  - PETROVA, Maria
AU  - DIMITROVA, Lyudmila
AU  - STANCHEVA, Ira
AU  - GENEVA, Maria
AU  - ZAYOVA, Ely
PY  - 2013
JO  - Turkish Journal of Biology
VL  - 37
IS  - 1
SN  - 1300-0152
SP  - 106
EP  - 113
DB  - TRDizin
UR  - http://search/yayin/detay/140769
ER  -