OBJECTIVE: To assess the efficiency of dequalinium chloride for treatment of vaginal infections of varying etiology. STUDY DESIGN: 100 women applying to gynecology outpatient clinic with vaginal discharge complaints and accompanying symptoms were enrolled prospectively into the study. All of the patients had a clini- cal and a microbiological diagnosis before the treatment. Patients were treated only with 10 mg de- qualinium chloride vaginal tablet once a day for 6 days. 10 days after the treatment, patients were re- examined and control cultures were taken for microbiologic examination. Clinical diagnosis, microbio- logic results and symptoms of patients before and after the treatment were compared. McNemar test and descriptive statistical analysis tests were applied for statistical analysis and significance level was accepted as p<0.05. RESULTS: 91 patients had a clinical vaginitis diagnosis before the treatment, 51 of them were cured with the dequalinium treatment (p<0.0001). Sixty patients had a microbiological diagnosis before the treatment, 46 of them were cured with the dequalinium treatment (p<0.001). Also, patients symptoms (bad odor, itching, burning, dysparonia) and vaginal discharge complaints were improved significantly after the treatment (p<0.001). CONCLUSION: Dequalinium chloride with its broad antimicrobial spectrum constitutes a valuable alternative to other vaginal anti-infective therapies.