Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia

KURÇENLİ, Saadet; ATALAY, Sacide; SINGER, Ralfi; kayadibi, huseyin; YEKREK, Mehmet Murat

Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia

Sacide ATALAY, (SB Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, Klinik Biyokimya Kliniği, İstanbul, Türkiye)

Ralfi SINGER, (SB Zeynep Kamil Kadın ve Doğum Hastanesi, Klinik Biyokimya Kliniği, İstanbul, Türkiye)

Hüseyin KAYADİBİ, (İskenderun Askeri Hastanesi, Klinik Biyokmya Kliniği, İskenderun, Türkiye)

Mehmet Murat YEKREK, (SB Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, Klinik Biyokimya Kliniği, İstanbul, Türkiye)

Saadet KURÇENLİ (SB Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, Klinik Biyokimya Kliniği, İstanbul, Türkiye)

Kafkas Tıp Bilimleri Dergisi

3 0

Yıl: 2011 Cilt: 1 Sayı: 2 Sayfa Aralığı: 47 - 52 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia

Öz:

AMAÇ: Serumda LDL-C ve sdLDL-C ölçümü için basit ve doğru bir metot gereklidir. Sentinel ve Vitros direkt LDL-C ölçüm metodlarının analitik performanslarını değerlendirmeyi, bu iki direkt ölçüm metodunun LDL-C değerlerini birbirleriyle ve Friedewald formülü ile hesaplanan LDL-C değerleri ile karşılaştırmayı ve hiperlipidemili hastaların sınıflandırılmasında hangi lipit parametresinin daha uygun olacağını belirlemeyi amaçladık.YÖNTEMLER: Direkt metotların analitik performansları 60 serum örneğinde değerlendirildi. İki farklı direkt LDL-C ölçüm metodu ve Friedewald formülü ile farklı 122 serum örneğinde LDL-C hesaplandı. Bunların dışındaki 118 serum örneğinde Sentinel sdLDL-C, Vitros sdLDL-C ve diğer değerlendirilen lipit parametreleri ölçüldü. BULGULAR: Sentinel direkt LDL-C, Vitros direkt LDL-C ve Friedewald formülü ile hesaplanan ortalama LDL-C konsantrasyonları sırasıyla 152±44 mg/dL, 146±45 mg/dL ve 141±41 mg/dL’ydi, (P<0.001). Friedewald LDL-C ile Sentinel dLDL-C ve Vitros dLDL-C arasında oldukça yüksek korelasyonlar gözlendi (sırasıyla, r=0,934 ve r=0,936). Direkt metodların çalışma içi tekrarlanabilirlikleri <%1,42 iken, toplam tekrarlanabilirlikleri Sentinel dLDL-C için <%1,73, Vitros dLDL-C için <%4,8 idi. Sentinel ve Vitros dLDL-C ölçüm metotlarının sistematik hataları sırasıyla %11 ve %17,5 idi. Hiperlipidemi grupları Friedewald LDL-C baz alınarak oluşturulmasına rağmen, en düşük Friedewald LDL-C hipertrigliseridemik grupta, en düşük sdLDL-C ise normolipidemik grupta gözlendi.SONUÇ: Vitros dLDL-C için toplam tekrarlanabilirlik >%4 olduğu için bu metodun NCEP ATP III performans kriterlerini karşılayamadığı söylenebilir. Friedewald formülü ile hesaplanan LDL-C değerleri ile karşılaştırıldığında direk ölçüm metodlarındaki LDL-C değerleri anlamlı şekilde yüksekti. Hiperlipidemi hastalarının değerlendirilmesinde Sentinel direkt LDL-C ya da sdLDL-C’ün tercih edilmesi daha faydalı olabilir.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Sentinel ve vitros direkt LDL-C ölçüm yöntemlerinin analitik performansları ve hiperlipidemi sınıflaması

Öz:

OBJECTIVE: A simple and accurate method is necessary to measure the LDL-C and sdLDL-C in serum. We aimed to evaluate the analytical performances of Sentinel and Vitros direct LDL-C (dLDL-C) assay methods, to compare LDL-C values of these direct methods with each other and with those values calculated by Friedewald formula, and to determine which lipid parameters could be more proper for classification of patients with hyperlipidemia. METHODS: Analytical performances of direct methods were evaluated in 60 serum samples. LDL-C was determined in different 122 sera via two different direct methods and Friedewald formula. Sentinel sdLDL-C, Vitros sdLDL-C and other evaluated lipid parameters were estimated in additional 118 serum samples. RESULTS: Mean LDL-C concentrations for Sentinel dLDL-C, Vitros dLDL-C and Friedewald formula were 152±44 mg/dL, 146±45 mg/dL, 141±41 mg/dL, respectively, (P<0.001). Highly significant associations were observed between Friedewald LDL-C and both Sentinel and Vitros dLDL-C (r=0.934, r=0.936, respectively). Although within-run imprecisions for direct methods were lower than 1.42%, total imprecisions for Sentinel and Vitros dLDL-C were lower than 1.73% and 4.8%, respectively. Sentinel and Vitros dLDL-C assay methods had 11% and 17.5% systematic error, respectively. While the lowest Friedewald LDL-C concentrations were observed in hypertriglyceridemic group, the lowest sdLDL-C values were obtained in normolipidemic group, although hyperlipidemia groups were based on Friedewald LDL-C. CONCLUSION: Vitros dLDL-C did not seem to be able to meet performance criterion of NCEP ATP III for LDL-C, because total imprecision for it was more than 4%. Direct assay methods significantly overestimated LDL-C values compared with Friedewald formula. Preference of the Sentinel direct LDL-C or sdLDL-C could be more useful for evaluation of patients with hyperlipidemia.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Kingsburry KJ, Bondy G. Understanding the essentials of lipid metabolism. Prog Cardiovasc Nurs 2003; 18: 13-8.
2. MRC/BHF Heart Protection Study of cholesterol lowering with simvastatin in 20,536 high-risk individuals: a randomised placebo-controlled trial. Lancet 2002; 360: 7-22.
3. Executive summary of the third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation and treatment of high blood cholesterol in adults (Adult Treatment Panel III). JAMA 2001; 285: 2486-97.
4. Kannel WB. Range of serum cholesterol values in the population developing coronary artery disease. Am J Cardiol 1995; 76: 69C-77C.
5. Christenson RH. National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines for utilization of biochemical markers in acute coronary syndromes and heart failure. Clin Chem 2007; 53: 545-6.
6. St-Pierre AC, Ruel IL, Cantin B, et al. Comparison of various electrophoretic characteristics of LDL particles and their relationship to the risk of ischemic heart disease. Circulation 2001; 104: 2295-9.
7. Hirano T, Ito Y, Saegusa H, et al. A novel and simple method for quantifi cation of small, dense LDL. J Lipid Res 2003; 44: 2193-201.
8. Koba S, Hirano T, Yoshino G, et al. Remarkably high prevalence of small dense low-density lipoprotein in Japanese men with coronary artery disease, irrespective of the presence of diabetes. Atherosclerosis 2002; 160: 249-56.
9. Mohan V, Deepa R, Velmurugan K, et al. Association of small dense LDL with coronary artery disease and diabetes in urban Asian Indians- the Chennai Urban Rural Epidemiology Study (CURES-8). J Assoc Physcians India 2005; 53: 95-100.
10. Packard CJ. Triacylglycerol-rich lipoproteins and the generation of small, dense low-density lipoprotein. Biochem Soc Trans 2003; 31: 1066-9.
11. Friedewald WT, Levy RI, Frederickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502.

APA	ATALAY S, SINGER R, kayadibi h, YEKREK M, KURÇENLİ S (2011). Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. , 47 - 52.
Chicago	ATALAY Sacide,SINGER Ralfi,kayadibi huseyin,YEKREK Mehmet Murat,KURÇENLİ Saadet Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. (2011): 47 - 52.
MLA	ATALAY Sacide,SINGER Ralfi,kayadibi huseyin,YEKREK Mehmet Murat,KURÇENLİ Saadet Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. , 2011, ss.47 - 52.
AMA	ATALAY S,SINGER R,kayadibi h,YEKREK M,KURÇENLİ S Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. . 2011; 47 - 52.
Vancouver	ATALAY S,SINGER R,kayadibi h,YEKREK M,KURÇENLİ S Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. . 2011; 47 - 52.
IEEE	ATALAY S,SINGER R,kayadibi h,YEKREK M,KURÇENLİ S "Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia." , ss.47 - 52, 2011.
ISNAD	ATALAY, Sacide vd. "Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia". (2011), 47-52.

APA	ATALAY S, SINGER R, kayadibi h, YEKREK M, KURÇENLİ S (2011). Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. Kafkas Tıp Bilimleri Dergisi, 1(2), 47 - 52.
Chicago	ATALAY Sacide,SINGER Ralfi,kayadibi huseyin,YEKREK Mehmet Murat,KURÇENLİ Saadet Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. Kafkas Tıp Bilimleri Dergisi 1, no.2 (2011): 47 - 52.
MLA	ATALAY Sacide,SINGER Ralfi,kayadibi huseyin,YEKREK Mehmet Murat,KURÇENLİ Saadet Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. Kafkas Tıp Bilimleri Dergisi, vol.1, no.2, 2011, ss.47 - 52.
AMA	ATALAY S,SINGER R,kayadibi h,YEKREK M,KURÇENLİ S Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. Kafkas Tıp Bilimleri Dergisi. 2011; 1(2): 47 - 52.
Vancouver	ATALAY S,SINGER R,kayadibi h,YEKREK M,KURÇENLİ S Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia. Kafkas Tıp Bilimleri Dergisi. 2011; 1(2): 47 - 52.
IEEE	ATALAY S,SINGER R,kayadibi h,YEKREK M,KURÇENLİ S "Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia." Kafkas Tıp Bilimleri Dergisi, 1, ss.47 - 52, 2011.
ISNAD	ATALAY, Sacide vd. "Analytical performances of sentinel and vitros direct LDL-C assay methods, and classification of hyperlipidemia". Kafkas Tıp Bilimleri Dergisi 1/2 (2011), 47-52.