Evaluation of serological diagnostic tests for human Brucellosis in an endemic area

OLDACAY, Mehmet; ARABACI, Filiz

Evaluation of serological diagnostic tests for human Brucellosis in an endemic area

Filiz ARABACI, (Sağlık Bakanlığı, Çanakkale Devlet Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, Çanakkale, Türkiye)

Mehmet OLDACAY (Sağlık Bakanlığı, Çanakkale Devlet Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, Çanakkale, Türkiye)

Journal of Microbiology and Infectious Diseases

4 0

Yıl: 2012 Cilt: 2 Sayı: 2 Sayfa Aralığı: 50 - 56 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of serological diagnostic tests for human Brucellosis in an endemic area

Öz:

Amaç: Bruselloz tanısında kullanılan serolojik testlerin klinik kullanılışlılığı çoğu durumlarda açık değildir. Bu çalışma Türkiye’nin endemik bir bölgesinde bu testlerin bruselloz için değerini araştırmayı amaç edindi. Gereç ve yöntem: Bu çalışma Çanakkale Devlet Hastanesinde 2009 yılında yapıldı. Retrospektif bir yaklaşımla bruselloz için araştırılan hastaların kayıtları toplandı. Çalışma süresinde 236 kişi (131 semptomatik, 105 semptomatik olmayan) brusella tanısı için araştırıldı. Tüm hastalardan alınan örnekler Rose Bengal lam testi, STA testi, Brusella Coombs testi, BrucellaCapt ve brusella IgG ve IgM kitleri ile brusella antikor seropozitifliği için test edildi. Bulgular: Bunların 49’u hastanede yatırıldı ve kan kültürleri alındı. Bunlardan dokuzunda Brucella spp. izole edildi (%18,4). Bruselloz için en duyarlı test BrucellaCapt testi idi (%74,0) ve onu çok yakın bir değerle Coombs testi (%72,5) takip ediyordu. RB testinin duyarlılığı %48,1 bulundu. ELISA IgG testi ELISA IgM testine göre daha duyarlı idi (sırasıyla, %65,6’e karşı %49, 6). Tüm çalışılan testler bruselloz için %100 özgül bulundu ancak RB testinin özgüllüğü %96,1 idi. Yapılan tüm brusella testlerinin pozitif prediktivitesi 1 olmakla beraber; negatif prediktivite sadece Coombs ve BrucellaCapt testi için anlamlı bulundu (sırasıyla 0,744 ve 0,755). Diğer serolojik testler 0,50 civarında veya daha aşağı idi ve bu değerler negatif sonuçlar için zayıftı. Sonuç: ELISA IgG ve IgM test sonuçları diğer testlere üstün bulunmadı. İstatistik ROC eğrisi analizine göre Brusella Coombs ve BrucellaCapt testi endemik sahada en değerli serolojik testler olarak bulundu. Semptomlu hastalarda serolojik testler negatif ise bu araştırılmalı ve kısa tekrarlanmalıdır.

Anahtar Kelime:

Konular: Mikrobiyoloji Enfeksiyon Hastalıkları

Endemik bölgede insan Brusellozu için Serolojik tanı testlerinin değerlendirilmesi

Öz:

Objectives: The clinical utility of complementary tests for brucellosis are not clear in many situation. This study aimed to evaluate value of these tests for brucellosis in an endemic area in Turkey.Materials and methods: This study was performed at Çanakkale General Hospital in 2009. In a retrospective approach, records of the patients who evaluated for brucellosis were collected. During the study period, 236 people (131 symptomatic and 105 non-symptomatic) were evaluated for diagnosis of brucellosis. All of the samples from these patients were tested for Brucella antibody seropositivity by RB slide agglutination, standard serum agglutination, Brucella Coombs, BrucellaCapt, and ELISA IgG and IgM tests. Results: In total, 49 symptomatic patients were hospitalized and blood cultures were obtained. Brucella spp. were isolated from nine of them (18.4%).The BrucellaCapt test was found to be the most sensitive for Brucella (74.0%) and close behind it was the Coombs test (72.5%). The sensitivity for the RB test was 48.1%. The ELISA IgG test was found more sensitive for brucellosis than the ELISA IgM test was (65.6% and 49.6%, respectively). All examined tests were found about 100% specific for brucellosis but the RB test was found less specific than the others were (96.1%) Positive predictive value for all tests was about 1 but negative predictive values were only valuable for the Coombs and Brucella Capt test (0.744 and 0.755, respectively). The other serological tests were around and below 0.50, which was weak for negative results.Conclusions: The ELISA IgG and IgM tests were no superior to the other tests. By assessment of receiver operating characteristics (ROC) analysis, the Brucella Coombs and BrucellaCapt tests were found to be the most valuable tests for serological diagnosis of brucellosis in endemic areas. The seronegative tests in the symptomatic patients should be evaluated and repeated in short time.

Anahtar Kelime:

Konular: Mikrobiyoloji Enfeksiyon Hastalıkları

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ARABACI F, OLDACAY M (2012). Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. , 50 - 56.
Chicago	ARABACI Filiz,OLDACAY Mehmet Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. (2012): 50 - 56.
MLA	ARABACI Filiz,OLDACAY Mehmet Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. , 2012, ss.50 - 56.
AMA	ARABACI F,OLDACAY M Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. . 2012; 50 - 56.
Vancouver	ARABACI F,OLDACAY M Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. . 2012; 50 - 56.
IEEE	ARABACI F,OLDACAY M "Evaluation of serological diagnostic tests for human Brucellosis in an endemic area." , ss.50 - 56, 2012.
ISNAD	ARABACI, Filiz - OLDACAY, Mehmet. "Evaluation of serological diagnostic tests for human Brucellosis in an endemic area". (2012), 50-56.

APA	ARABACI F, OLDACAY M (2012). Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. Journal of Microbiology and Infectious Diseases, 2(2), 50 - 56.
Chicago	ARABACI Filiz,OLDACAY Mehmet Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. Journal of Microbiology and Infectious Diseases 2, no.2 (2012): 50 - 56.
MLA	ARABACI Filiz,OLDACAY Mehmet Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. Journal of Microbiology and Infectious Diseases, vol.2, no.2, 2012, ss.50 - 56.
AMA	ARABACI F,OLDACAY M Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. Journal of Microbiology and Infectious Diseases. 2012; 2(2): 50 - 56.
Vancouver	ARABACI F,OLDACAY M Evaluation of serological diagnostic tests for human Brucellosis in an endemic area. Journal of Microbiology and Infectious Diseases. 2012; 2(2): 50 - 56.
IEEE	ARABACI F,OLDACAY M "Evaluation of serological diagnostic tests for human Brucellosis in an endemic area." Journal of Microbiology and Infectious Diseases, 2, ss.50 - 56, 2012.
ISNAD	ARABACI, Filiz - OLDACAY, Mehmet. "Evaluation of serological diagnostic tests for human Brucellosis in an endemic area". Journal of Microbiology and Infectious Diseases 2/2 (2012), 50-56.