Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains

ONUK, ERTAN EMEK; Ciftci, Alper; ALTUN, Soner; duman, Muhammed; BÜYÜKEKİZ, Ayşe Gül

Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains

Soner ALTUN, (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

Ertan Emek ONUK, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

ALPER ÇİFTCİ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Ayşe Gül BÜYÜKEKİZ, (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

Muhammed DUMAN (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

3 1

Yıl: 2013 Cilt: 19 Sayı: 3 Sayfa Aralığı: 375 - 381 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains

Öz:

Araştırmada, gökkuşağı alabalığı işletmelerinden izole edilmiş olan 10 adet Lactococcus garvieae suşunun 3 adet referans suşla (İspanya, İngiltere ve ATCC 43921) karşılaştırmalı olarak fenotipik ve genotipik özellikleri incelenmiştir. L. garvieae suşlarının fenotipik özelliklerinin belirlenmesinde konvansiyonel mikrobiyolojik ve Rapid 32 STREP testleri kullanılmıştır. Rapid 32 STREP sistemde suşlar arasında β-Glucuronidase, D-ribose, sorbitol, lactose, raffinose, alanyl-phenylalanyl-proline arylamidase, pyrrolidonyl arylamidase, hippurate hydrolysis, urease testleri yönüyle farklıklar olmasına rağmen API Webde tüm suşlar L. garvieae olarak doğrulanmıştır. ERIC2 primerinin kullanıldığı RAPD PCR analizinde L. garveae izolatları %70 benzerlik katsayısına göre 3 farklı genotipe ayrılmış ve Türkiye kökenli izolatların büyük bir bölümü (8 izolat) predominant tip olan LG1 genotipine dahil olduğu belirlenmiştir. Ayrıca bu çalışmada L. garvieae suşlarının antibiyotik duyarlılık testlerinde ülkemizde balıklarda sıklıkla kullanılan gentamisin, neomycin, lincomycin, sulphamethoxazoletrimetoprim, oksitetrasiklin, eritromycin, amoxycillin, florfenikol ve doksisikline karşı direnç geliştirmiş oldukları belirlenmiştir.

Anahtar Kelime: ilaç direnci oksitetrasiklin gentamisin florfenikol fenotip Lactococcus garvieae sucul organizmalar sulfametazol direnç mekanizmaları çoklu ilaç direnci amoksilin gökkuşağı alabalığı neomisin doksisiklin alabalık eritromisin genotip trimetoprim ilaç tedavisi sucul hayvanlar duyarlılık linkomisin bakteriyel hastalıklar

Konular: Veterinerlik

Lactococcus garvieae suşlarının fenotipik, genotipik karakterizasyonu ve antimikrobiyal duyarlılıklarının belirlenmesi

Öz:

In this study, phenotypic and genotypic features of 10 L. garvieae strains isolated from rainbow trout farms were examined with 3 reference strains (Spain, England and ATCC 43921) comperativly. Rapid 32 STREP and conventional microbiologic tests were used for determining phenotipic features of L. garvieae strains. Altough there are diff erences, in Rapid 32 STREP system, between strains in terms of β-Glucuronidase, D-ribose, sorbitol, lactose, raffinose, alanyl-phenylalanyl-proline arylamidase, pyrrolidonyl arylamidase, hippurate hydrolysis, urease tests, all strains have been confirmed as L. garvieae by API web. In RAPD PCR analysis, in which ERIC 2 primer is used, L. garvieae isolates were genotyped within 3 separate clusters according to similarity coefficient index of 70%, and it was detected that a vast majority of isolates with Turkey-origin (8 isolates) belongs to predominant type LG1 genotype. In addition to this, antimicrobial tests of L. garvieae strains shows that there are resistance against gentamycin, neomycin, lincomycin, sulfamethoxazole-trimethoprim, oxytetracycline, erythromycin, amoxicillin, florfenicol and doxycycline, which are frequently used on fish in our country.

Anahtar Kelime: amoxicillin rainbow trout neomycin doxycycline trout erythromycin genotypes trimethoprim drug therapy aquatic animals susceptibility lincomycin bacterial diseases drug resistance oxytetracycline gentamicin florfenicol phenotypes Lactococcus garvieae aquatic organisms sulfamethoxazole resistance mechanisms multiple drug resistance

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ALTUN S, ONUK E, Ciftci A, BÜYÜKEKİZ A, duman M (2013). Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. , 375 - 381.
Chicago	ALTUN Soner,ONUK ERTAN EMEK,Ciftci Alper,BÜYÜKEKİZ Ayşe Gül,duman Muhammed Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. (2013): 375 - 381.
MLA	ALTUN Soner,ONUK ERTAN EMEK,Ciftci Alper,BÜYÜKEKİZ Ayşe Gül,duman Muhammed Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. , 2013, ss.375 - 381.
AMA	ALTUN S,ONUK E,Ciftci A,BÜYÜKEKİZ A,duman M Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. . 2013; 375 - 381.
Vancouver	ALTUN S,ONUK E,Ciftci A,BÜYÜKEKİZ A,duman M Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. . 2013; 375 - 381.
IEEE	ALTUN S,ONUK E,Ciftci A,BÜYÜKEKİZ A,duman M "Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains." , ss.375 - 381, 2013.
ISNAD	ALTUN, Soner vd. "Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains". (2013), 375-381.

APA	ALTUN S, ONUK E, Ciftci A, BÜYÜKEKİZ A, duman M (2013). Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(3), 375 - 381.
Chicago	ALTUN Soner,ONUK ERTAN EMEK,Ciftci Alper,BÜYÜKEKİZ Ayşe Gül,duman Muhammed Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19, no.3 (2013): 375 - 381.
MLA	ALTUN Soner,ONUK ERTAN EMEK,Ciftci Alper,BÜYÜKEKİZ Ayşe Gül,duman Muhammed Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.19, no.3, 2013, ss.375 - 381.
AMA	ALTUN S,ONUK E,Ciftci A,BÜYÜKEKİZ A,duman M Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(3): 375 - 381.
Vancouver	ALTUN S,ONUK E,Ciftci A,BÜYÜKEKİZ A,duman M Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(3): 375 - 381.
IEEE	ALTUN S,ONUK E,Ciftci A,BÜYÜKEKİZ A,duman M "Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19, ss.375 - 381, 2013.
ISNAD	ALTUN, Soner vd. "Phenotypic, genotypic characterisation and antimicrobial susceptibility determination of lactococcus garvieae strains". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19/3 (2013), 375-381.