Yıl: 2013 Cilt: 19 Sayı: 3 Sayfa Aralığı: 517 - 521 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese

Öz:
Bu çalışmada Erzincan tulum peynirinden izole edilen Staphylococcus aureusların biyofilm oluşturabilme yetenekleri ve antibiyotik dirençlilikleri fenotipik ve genotipik yöntemlerle araştırıldı. Araştırmada 100 adet Erzincan tulum peyniri numunesi toplandı. Peynir örneklerinden Staphylococcus aureus izolasyonu için Baird-Parker agara ekim yapıldı. İzolatlar Gram boyama, hemoliz ve koagülaz testleriyle S. aureus olarak identifiye edildi. Polimeraz Zincir Reaksiyonu ile izolatlar nuc, mecA, vanA ve vanB genleri yönünden incelendi. S. aureusların biyofilm oluşturma yeteneği için Kongo Red agar ve oksasilin (1 μg), sefoksitin (30 μg), vankomisin (30 μg), amoksisilin-klavulonik asit (20 μg) ve penisilin (10 unit) diskleri antibiyotik dirençliliğin saptanması amacıyla kullanıldı. Test edilen örneklerin 72 (%72)si Staphylococcus spp. pozitif bulundu. Bunlardan 61 (%84.7) örnek fenotipik ve genotipik olarak S. aureus olarak identifiye edildi. İzolatların 37 (%60.6)sinin biyofilm oluşturduğu saptandı. Disk difüzyon yönteminde 61 izolattan 9 (%14.7)unda oksasilin (metisilin), 8 (%13.1)inde sefoksitin, 4 (%6.5)ünde amoksisilin-klavulonik asit, 28 (%45.9)inde ise penisilin dirençliliği tespit edilirken vankomisin dirençli izolata rastlanmadı. Polimeraz Zincir Reaksiyonu sonucunda 61 örnekten 10 (%16.3)unda nuc ve mecA genleri saptanırken vanA ve vanB genlerine rastlanmadı. Bu araştırmada Erzincan tulum peynirinde S. aureusun yüksek bir oranda bulunduğu görülmüştür. Kontaminasyon oranının yüksek olması ve izolatların antibiyotik dirençliliği göz önünde bulundurulduğunda hijyenik şartlarda üretilmeyen bu peynirlerin halk sağlığı açısından büyük risk oluşturabileceği kanaatine varılmıştır.
Anahtar Kelime: Staphylococcus aureus ilaç direnci Erzincan peynir kalitesi fenotip tulum peyniri kan yıkımı oksasilin vankomisin peynir penisilinler mikrobiyal bulaşıklık gıda bulaşıklılığı gıda güvenirliği direnç mekanizmaları gıda kaynaklı hastalıklar gıda hijyeni antibiyotikler amoksilin koagülaz testi Türkiye biyofilm genotip risk faktörleri genler klavulanik asit sefoksitin

Konular: Veterinerlik

Erzincan tulum peynirinden izole edilen Staphylococcus aureus izolatlarında antibiyotik direncinin ve biyofilm oluşturma özelliğinin fenotipik ve genotipik olarak belirlenmesi

Öz:
In this study, biofilm formation and antibiotic resistance of Staphylococcus aureus strains isolated from Erzincan tulum cheeses were phenotypically and genotypically investigated. Samples collected from 100 different Erzincan tulum cheese were inoculated into Baird-Parker agar to isolate S. aureus. Identification of S. aureus was performed with Gram staining, hemolysis or coagulase tests. Determination of the nuc, mecA, vanA, and vanB genes in isolates was performed by Polymerase Chain Reaction (PCR). Congo red agar was used for the biofilm formation of S. aureus. Antibiotic resistance was determined by antibiotic discs of oxacillin (1 μg), cefoxitin (30 μg), vancomycin (30 μg), amoxicillin-clavulanic acid (20 μg), and penicillin (10 units). A total of 72 of 100 (72%) samples were positive for Staphylococcus spp. Of 72 samples, 61 (84.7%) were phenotypically and genotypically identified as S. aureus. Of 61 isolates, 37 (60.6%) formed a biofilm. Of 61 isolates, 49 were determined to resistant to antibiotics of oxacillin (methicillin) (9), cefoxitin (8), amoxicillin-clavulanic acid (4), and, penicillin (28). Vancomycin-resistance was not detected. Only the nuc and mecA genes were detected in 10 of 61 (16.3%) strains of S. aureus. In this study, the rate of S. aureus determined in Erzincan tulum cheeses was high. Considering the high rate of contamination and antibiotic resistance due to poor hygienic conditions, it was concluded that Erzincan tulum cheese, now a PDO cheese, should be considered to be great risk for public health.
Anahtar Kelime: antibiotics amoxicillin coagulase test Turkey biofilms genotypes risk factors genes clavulanic acid cefoxitin Staphylococcus aureus drug resistance Erzincan cheese quality phenotypes tulum cheese haemolysis oxacillin vancomycin cheeses penicillins microbial contamination food contamination food safety resistance mechanisms foodborne diseases food hygiene

Konular: Veterinerlik
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ÖZPINAR N, Gümüşsoy K (2013). Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. , 517 - 521.
Chicago ÖZPINAR NECATİ,Gümüşsoy Kadir Semih Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. (2013): 517 - 521.
MLA ÖZPINAR NECATİ,Gümüşsoy Kadir Semih Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. , 2013, ss.517 - 521.
AMA ÖZPINAR N,Gümüşsoy K Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. . 2013; 517 - 521.
Vancouver ÖZPINAR N,Gümüşsoy K Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. . 2013; 517 - 521.
IEEE ÖZPINAR N,Gümüşsoy K "Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese." , ss.517 - 521, 2013.
ISNAD ÖZPINAR, NECATİ - Gümüşsoy, Kadir Semih. "Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese". (2013), 517-521.
APA ÖZPINAR N, Gümüşsoy K (2013). Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(3), 517 - 521.
Chicago ÖZPINAR NECATİ,Gümüşsoy Kadir Semih Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19, no.3 (2013): 517 - 521.
MLA ÖZPINAR NECATİ,Gümüşsoy Kadir Semih Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.19, no.3, 2013, ss.517 - 521.
AMA ÖZPINAR N,Gümüşsoy K Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(3): 517 - 521.
Vancouver ÖZPINAR N,Gümüşsoy K Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(3): 517 - 521.
IEEE ÖZPINAR N,Gümüşsoy K "Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19, ss.517 - 521, 2013.
ISNAD ÖZPINAR, NECATİ - Gümüşsoy, Kadir Semih. "Phenotypic and genotypic determination of antibiotic resistant and biofilm forming Staphylococcus aureus isolated in Erzincan tulum cheese". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19/3 (2013), 517-521.