Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods

VARDAR, Fazilet; Gezgin, Yüksel; ELTEM, Rengin; SARGIN, Sayit

Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods

Sayit SARGIN, (Ege Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, İzmir, Türkiye)

Yüksel GEZGİN, (Ege Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, İzmir, Türkiye)

Rengin ELTEM, (Ege Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, İzmir, Türkiye)

Fazilet VARDAR (Ege Üniversitesi, Mühendislik Fakültesi, Biyomühendislik Bölümü, İzmir, Türkiye)

Turkish Journal of Biology

9 0

Yıl: 2013 Cilt: 37 Sayı: 2 Sayfa Aralığı: 139 - 146 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods

Öz:

In this study, Trichoderma harzianum EGE-K38 was used for micropropagule production in solid-state fermentation. Various inexpensive agricultural co-products including wheat bran, sawdust, rice straw, hazelnut shell, grape marc, and cotton seed cake were tested as natural substrates, and zeolite was tested as an inert support for micropropagule production. Several parameters were determined to obtain maximum micropropagule production: the effects of various nitrogen sources, initial moisture content, initial pH, incubation temperature, and incubation time. Maximum micropropagule count achieved was (1.30 ± 0.68) × 1010 cfu/g dry substrate with a wheat bran malt sprout mixture (3:2), at 28 °C, an initial moisture content of 70% (w/w), and an initial pH of 5.8 ± 0.3 after 4 days of cultivation. In zeolite-based medium, a maximum micropropagule count of (5.12 ± 0.61) × 108 cfu/g dry inert support was achieved. Optimized parameters were used in a higher scale where micropropagules were produced in trays using a wheat bran malt sprout medium and harvested to determine the drying conditions in both lyophilization and oven-drying. It was found that up to 97% of micropropagules retained their viability with glycerol as a protectant after 1 day of oven-drying at 40 °C.

Anahtar Kelime: comparisons zeolites biological control fermentation micropropagation Trichoderma harzianum

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SARGIN S, Gezgin Y, ELTEM R, VARDAR F (2013). Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. , 139 - 146.
Chicago	SARGIN Sayit,Gezgin Yüksel,ELTEM Rengin,VARDAR Fazilet Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. (2013): 139 - 146.
MLA	SARGIN Sayit,Gezgin Yüksel,ELTEM Rengin,VARDAR Fazilet Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. , 2013, ss.139 - 146.
AMA	SARGIN S,Gezgin Y,ELTEM R,VARDAR F Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. . 2013; 139 - 146.
Vancouver	SARGIN S,Gezgin Y,ELTEM R,VARDAR F Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. . 2013; 139 - 146.
IEEE	SARGIN S,Gezgin Y,ELTEM R,VARDAR F "Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods." , ss.139 - 146, 2013.
ISNAD	SARGIN, Sayit vd. "Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods". (2013), 139-146.

APA	SARGIN S, Gezgin Y, ELTEM R, VARDAR F (2013). Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology, 37(2), 139 - 146.
Chicago	SARGIN Sayit,Gezgin Yüksel,ELTEM Rengin,VARDAR Fazilet Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology 37, no.2 (2013): 139 - 146.
MLA	SARGIN Sayit,Gezgin Yüksel,ELTEM Rengin,VARDAR Fazilet Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology, vol.37, no.2, 2013, ss.139 - 146.
AMA	SARGIN S,Gezgin Y,ELTEM R,VARDAR F Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology. 2013; 37(2): 139 - 146.
Vancouver	SARGIN S,Gezgin Y,ELTEM R,VARDAR F Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods. Turkish Journal of Biology. 2013; 37(2): 139 - 146.
IEEE	SARGIN S,Gezgin Y,ELTEM R,VARDAR F "Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods." Turkish Journal of Biology, 37, ss.139 - 146, 2013.
ISNAD	SARGIN, Sayit vd. "Micropropagule production from Trichoderma harzianum EGE-K38 using solid-state fermentation and a comparative study for drying methods". Turkish Journal of Biology 37/2 (2013), 139-146.