Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey

SAKRU, NERMİN; ERYILDIZ, Canan

Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey

Canan ERYILDIZ, (SB Tokat Devlet Hastanesi, Tıbbi Mikrobiyoloji Kliniği, Tokat, Türkiye)

Nermin ŞAKRU (Trakya Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Edirne, Türkiye)

Balkan Medical Journal

3 2

Yıl: 2012 Cilt: 29 Sayı: 3 Sayfa Aralığı: 261 - 267 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey

Öz:

Amaç: Echinococcus granulosus insanlarda ve birçok evcil hayvanda kistik ekinokokkozisin etkenidir ve dünya çapında önemli bir halk sağlığı sorunudur. Bu çalışmanın amacı Türkiye’nin Trakya bölgesinde insanlardan ve hayvanlardan elde edilen E. granulosus izolatlarının genotipini belirlemektir. Gereç ve Yöntemler: Edirne ilinde çeşitli hastanelerde opere edilen insanlardan ve kesimhanedeki hayvanlardan toplam 58 izolat elde edildi. E. granulosus’un insan ve hayvan izolatlarını karakterize etmek için; ribozomal birinci internal transcribed spacer fragmanının analizi için PCR-RFLP (polimeraz zincir reaksiyonu - restriksiyon fragman uzunluğu polimorfizmi) yöntemi ve parsiyel mitokondriyal NADH dehidrogenaz subunit 1 geninin analizi için PCR-SSCP (polimeraz zincir reaksiyonu - tek sarmal konformasyon polimorfizmi) yöntemi kullanıldı. İzolatların genetik özelliklerinin ileri incelemeleri için mitokondriyal sitokrom oksidaz c subunit 1 (CO1) ve NADH dehidrogenaz subunit 1 (ND1) gen bölgelerinin DNA (deoksiribonükleik asit) dizi analizi yapıldı. Bulgular: Kırk iki insan, 13 sığır ve 3 koyun kökenli olan 58 E. granulosus izolatı analiz edildi. Sonuçlar G1 (evcil koyun suşu) ve G7 (domuz suşu) olmak üzere iki farklı genotip bulunduğunu gösterdi. İnsan, koyun ve sığırları etkileyen E. granulosus’un en yaygın genotipinin koyun suşu olduğu gösterildi. Parsiyel mitokondriyal CO1 ve ND1 verileri birlikte değerlendirildiğinde mevcut çalışmadaki E. granulosus izolatlarında 8 haplotip tanımlandı. Sonuç: Bu çalışma; Trakya bölgesindeki insanlarda E. granulosus’un domuz suşunun varlığını gösteren ilk rapordur. Türkiye’de E. granulosus’un kontrol programları için yeni stratejiler oluşturulmasını önermekteyiz.

Anahtar Kelime:

Konular: Cerrahi

Öz:

Objective: Echinococcus granulosus is the causative agent of cystic echinococcosis in humans and many domestic animals, and remains an important global health problem. The aim of this study was to genotype E. granulosus isolates obtained from humans and animals in the Thrace Region of Turkey. Material and Methods: A total of 58 isolates were obtained from patients who underwent surgery at several hospitals and from animals at a slaughterhouse in the province of Edirne. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of ribosomal internal transcribed spacer 1 fragments, and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) of the partial mitochondrial NADH dehydrogenase subunit 1 (ND1) gene, was used to characterize human and animal E. granulosus isolates. To investigate the genetic characteristics of isolates, deoxyribonucleic acid (DNA) sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and ND1 genes was performed. Results: Fifty-eight E. granulosus isolates, including 42 from human, 13 from cattle and 3 from sheep were, analyzed. The results indicated two distinct genotypes: the G1 (sheep strain) and G7 (pig strain) genotypes. The sheep strain was shown to be the most common genotype of E. granulosus affecting humans, sheep and cattle. Among the concatenated partial CO1 and ND1 sequence data, eight haplotypes of Echinococcus species were identified in the present study. Conclusion: This is the first report indicating that the E. granulosus pig strain is present in humans in this region. We suggest that new strategies be designed for E. granulosus control programs in Turkey.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ERYILDIZ C, SAKRU N (2012). Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. , 261 - 267.
Chicago	ERYILDIZ Canan,SAKRU NERMİN Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. (2012): 261 - 267.
MLA	ERYILDIZ Canan,SAKRU NERMİN Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. , 2012, ss.261 - 267.
AMA	ERYILDIZ C,SAKRU N Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. . 2012; 261 - 267.
Vancouver	ERYILDIZ C,SAKRU N Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. . 2012; 261 - 267.
IEEE	ERYILDIZ C,SAKRU N "Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey." , ss.261 - 267, 2012.
ISNAD	ERYILDIZ, Canan - SAKRU, NERMİN. "Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey". (2012), 261-267.

APA	ERYILDIZ C, SAKRU N (2012). Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. Balkan Medical Journal, 29(3), 261 - 267.
Chicago	ERYILDIZ Canan,SAKRU NERMİN Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. Balkan Medical Journal 29, no.3 (2012): 261 - 267.
MLA	ERYILDIZ Canan,SAKRU NERMİN Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. Balkan Medical Journal, vol.29, no.3, 2012, ss.261 - 267.
AMA	ERYILDIZ C,SAKRU N Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. Balkan Medical Journal. 2012; 29(3): 261 - 267.
Vancouver	ERYILDIZ C,SAKRU N Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey. Balkan Medical Journal. 2012; 29(3): 261 - 267.
IEEE	ERYILDIZ C,SAKRU N "Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey." Balkan Medical Journal, 29, ss.261 - 267, 2012.
ISNAD	ERYILDIZ, Canan - SAKRU, NERMİN. "Molecular characterization of human and animal isolates of echinococcus granulosus in the thrace region, Turkey". Balkan Medical Journal 29/3 (2012), 261-267.