Effects of acrylamide treatment on oxidant and antioxidant levels in rats

Yener, Yeşim; TOKER, Aysun; YERLİKAYA, Fatma Hümeyra

Effects of acrylamide treatment on oxidant and antioxidant levels in rats

Fatma Hümeyra YERLİKAYA, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Biyokimya Anabilim Dalı, Konya, Türkiye)

Aysun TOKER, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Biyokimya Anabilim Dalı, Konya, Türkiye)

Yeşim YENER (Abant İzzet Baysal Üniversitesi, Eğitim Fakültesi, Fen Bilgisi Eğitimi Bölümü, Bolu, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2013 Cilt: 19 Sayı: 4 Sayfa Aralığı: 607 - 612 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of acrylamide treatment on oxidant and antioxidant levels in rats

Öz:

Bu çalışmada, uzun süre akrilamid verilen sıçanlar üzerinde total antioksidan durum (TAS), total oksidan durum (TOS) ve iskemi modifiye albuminin (IMA) serum düzeylerinin nasıl değiştiğinin araştırılması amaçlanmıştır. Çalışmada 65-75 g ağırlığında ve yaşları 3-4 haftalık 25 erkek ve 25 dişi Wistar cinsi sıçanlar kullanılmıştır. Hayvanlar 90 gün boyunca standart sıçan yemi ile beslenmişlerdir. Bununla beraber, günlük tüketecekleri içme suyuna 2 mg/kg/gün ve 5 mg/kg/gün dozunda akrilamid ilave edilmiştir. Akrilamid uygulaması sonrası hayvanlar anestezi altında servikal dislokasyonla öldürülmüş ve serumlarında IMA, TAS, TOS ve albumin düzeyleri spektrofotometrik yöntem ile ölçülmüştür. 2 mg/kg ve 5mg/kg akrilamid verilen erkek sıçanlara ait serum IMA düzeyleri kontrol grubuna göre önemli derecede yüksek bulunmuştur. Ayrıca, 5mg/kg akrilamid verilen erkek sıçanlara ait serum TAS düzeyleri kontrol grubuna göre önemli derecede düşük ve serum TOS değerleri önemli derecede yüksek bulunmuştur. 2 mg/kg ve 5mg/kg akrilamid verilen dişi sıçanlara ve kontrol grubuna ait serum IMA, TAS, TOS ve albumin düzeyleri arasında istatistiki açıdan önemli bir fark bulunamamıştır. Bu sonuçlara bağlı olarak bulgularımız, akrilamidin oksidatif stresi artırdığını göstermektedir.

Anahtar Kelime:

Konular: Veterinerlik

Ratlarda Akrilamid Kullanımının Antioksidan ve Oksidan Değerleri Üzerine Etkisi

Öz:

The aim of this study was to investigate serum total antioxidant status (TAS), total oxidant status (TOS) and ischemia-modified albumin (IMA) levels in long term acrylamide (ACR) given rats, compared to control rats. In total, 25 male and 25 female Wistar rats were involved in this experiment. Animals in each sex were segregated into three groups. Two of them were treatment groups and one of them was control group. Each treatment group consisted of ten animals and each control group consisted of five animals. ACR was administered to the treatment groups at 2 and 5 mg/kg/day via drinking water for 90 days. In the end of the experiment, serum samples were analyzed for IMA, TAS, TOS and albumin levels with the spectrophotometric method. Serum IMA and adjusted IMA levels were significantly higher at concentrations of 2 mg/kg and 5 mg/kg in the male rats when compared with those of the control male rats. Serum TAS levels significantly decreased at concentrations of 5 mg/kg in the male rats when compared with those of the control rats. We also observed a significant increase in the levels of serum TOS at concentrations of 5 mg/kg in the male rats. There were no significant differences between serum IMA, TAS, TOS and albumin levels at concentrations of 2 mg/kg and 5 mg/kg in the female rats. Our findings show that long term treatment with 2 mg/kg and 5 mg/kg doses of ACR led to a significant depletion of serum TAS levels and overproduction of serum TOS and IMA levels, consequently, to an increase in oxidative stress.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YERLİKAYA F, TOKER A, Yener Y (2013). Effects of acrylamide treatment on oxidant and antioxidant levels in rats. , 607 - 612.
Chicago	YERLİKAYA Fatma Hümeyra,TOKER Aysun,Yener Yeşim Effects of acrylamide treatment on oxidant and antioxidant levels in rats. (2013): 607 - 612.
MLA	YERLİKAYA Fatma Hümeyra,TOKER Aysun,Yener Yeşim Effects of acrylamide treatment on oxidant and antioxidant levels in rats. , 2013, ss.607 - 612.
AMA	YERLİKAYA F,TOKER A,Yener Y Effects of acrylamide treatment on oxidant and antioxidant levels in rats. . 2013; 607 - 612.
Vancouver	YERLİKAYA F,TOKER A,Yener Y Effects of acrylamide treatment on oxidant and antioxidant levels in rats. . 2013; 607 - 612.
IEEE	YERLİKAYA F,TOKER A,Yener Y "Effects of acrylamide treatment on oxidant and antioxidant levels in rats." , ss.607 - 612, 2013.
ISNAD	YERLİKAYA, Fatma Hümeyra vd. "Effects of acrylamide treatment on oxidant and antioxidant levels in rats". (2013), 607-612.

APA	YERLİKAYA F, TOKER A, Yener Y (2013). Effects of acrylamide treatment on oxidant and antioxidant levels in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(4), 607 - 612.
Chicago	YERLİKAYA Fatma Hümeyra,TOKER Aysun,Yener Yeşim Effects of acrylamide treatment on oxidant and antioxidant levels in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19, no.4 (2013): 607 - 612.
MLA	YERLİKAYA Fatma Hümeyra,TOKER Aysun,Yener Yeşim Effects of acrylamide treatment on oxidant and antioxidant levels in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.19, no.4, 2013, ss.607 - 612.
AMA	YERLİKAYA F,TOKER A,Yener Y Effects of acrylamide treatment on oxidant and antioxidant levels in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(4): 607 - 612.
Vancouver	YERLİKAYA F,TOKER A,Yener Y Effects of acrylamide treatment on oxidant and antioxidant levels in rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(4): 607 - 612.
IEEE	YERLİKAYA F,TOKER A,Yener Y "Effects of acrylamide treatment on oxidant and antioxidant levels in rats." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19, ss.607 - 612, 2013.
ISNAD	YERLİKAYA, Fatma Hümeyra vd. "Effects of acrylamide treatment on oxidant and antioxidant levels in rats". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19/4 (2013), 607-612.