Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats

KARAHAN, NERMİN; BAŞYİĞİT KILIÇ, GÜLDEN; ONARAN, İBRAHİM; SÜTÇÜ, Recep; ÇİRİŞ, İbrahim Metin; KOYU, Ahmet; ÇAM, Hakan; KARAHAN, Aynur G.; Keskin, Muharrem; İŞLER, Mehmet

Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats

Nermin KARAHAN, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Isparta, Türkiye)

Mehmet İŞLER, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Isparta, Türkiye)

Ahmet KOYU, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Psikiyatri Anabilim Dalı, Isparta, Türkiye)

Aynur G. KARAHAN, (Süleyman Demirel Üniversitesi, Gıda Mühendisliği Bölümü, Isparta, Türkiye)

Gülden Başyiğit KILIÇ, (Süleyman Demirel Üniversitesi, Isparta, Türkiye)

İbrahim Metin ÇİRİŞ, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Isparta, Türkiye)

Recep SÜTÇÜ, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Isparta, Türkiye)

İbrahim ONARAN, (Süleyman Demirel Üniversitesi, Isparta, Türkiye)

Hakan ÇAM, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Isparta, Türkiye)

Muharrem KESKİN (Süleyman Demirel Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Isparta, Türkiye)

Turkish Journal of Gastroenterology

14 2

Yıl: 2012 Cilt: 23 Sayı: 2 Sayfa Aralığı: 110 - 121 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats

Öz:

Amaç: Intestinal bakteriler, hepatik insülin direnci vc non-alkolik yağlı karaciğer hastalığında patojenik rol oynayan endojen sinyalleri indük-lerler. Probiyotikler barsak florasını düzenleyerek karaciğer-barsak ekseninde etki gösterebilirler. Bu çalışmada sıçan modellerinde metiyonin ve kolinden fakir diyet ile oluşturulan non-alkolik steatohepatitte iki probiyotik karışımının koruyucu etkisini araştırmayı amaçladık. Yöntem ve Gereç: Altmış adet erkek Wistar sıçan kullanılarak 2 ve 6 haftalık iki çalışma yapılmıştır, iki haftalık çalışma 6 gruptan oluşturulmuştur. Sıçanlar metiyonin ve kolinden fakir diyet ya da kontrol diyetle beslenmiş ve orogastrik sonda ile plasebo ya da iki probiyotik karışımı (Pro-1, Pro-2) verilmiştir. Altı haftalık çalışmada sıçanlar 4 gruba bölünerek metiyonin ve kolinden fakir diyet ya da kontrol diyet ile beslenmişler ve plasebo ya da Pro-2 verilmişlerdir. Çalışma sonunda sıçanlar kanları alınarak kurban edilmiş ve karaciğerleri alınmıştır. Serum alanin ami-notransferazlari saptanan hayvanlarda steatozis, inflanıasyon, tümör nekroz faktörü - a ekspresyonu ve apopitozisiıı saptanması için histolojik ve immünhistokimyasal inceleme yapılmıştır. Bulgular: Her iki çalışmada da metiyonin ve kolinden fakir diyet serum alanin anıinotransfe-raz seviyesinde, Pro-1 ve Pro-2 ile hafif dtişüriilebilen. bir artışa neden olmuştur, iki ve 6 haftalık çalışmalarda metiyonin ve kolinden fakir diyet tüm sıçanlarda steatozis ve inflamasyona neden olurken fibrozis oluşturmamıştır. İki haftalık çalışmada metiyonin ve kolinden fakir diyet ve Pro-1 ile beslenen 6 sıçanın 2'sinde steatozis ve inflanıasyon görülmüştür. Metiyonin ve kolinden fakir diyet ve Pro-2 ile beslenen 6 sıçanın 3'ünde steatozis görülürken 2'sinde inflanıasyon vardı. Altı haftalık çalışmada metiyonin ve kolinden fakir diyet ve Pro-2 ile beslenen 6 sıçanın 3'ünde steatozis ve inflanıasyon vardı. Her iki çalışmada da metiyonin ve kolinden fakir diyet tüm sıçan karaciğerlerinde, tümör nekroz faktö-rii-ct, proapopitotik Box, caspase 3, caspase 8 vc anti-apopitotik Bcl-2 ekspresyonuna neden oldu. Sonuç: Pro-1 ve Pro-2, sıçanlarda metiyonin ve kolinden fakir diyet ile oluşturulan steatohepatiti azaltmaktadır. Probiyotiklerin koruyucu etkisi kısmen apopitoz modülasyonu ve antiinfla-matuar etkilerine bağlı olabilir.

Anahtar Kelime:

Konular: Cerrahi

Sıçanlarda metiyonin ve kolinden fakir diyetle oluşturulan steatohepatitte probiyotiklerin etkisi

Öz:

Background/aims: Intestinal bacteria induce endogenous signals that play a pathogenic role in hepatic insulin resistance and non-alcoholic fatty liver disease. Probiotics could modulate the gut flora and could influence the gut-liver axis. We aimed to investigate the preventive effect of two probiotic mixtures on the methionine choline-deficient diet-induced non-alcoholic steatohepatitis model in rats. Methods: Two studies, shortterm (2 weeks) and long-term (6 weeks), were carried out using 60 male Wistar rats. The 2-week study included six groups. Rats were fed with methionine choline-deficient diet or pair-fed control diet and were given a placebo or one of two probiotic mixtures (Pro-1 and Pro-2) by orogastric gavage. In the 6-week study, rats were allocated into four groups and were fed with methionine choline-deficient diet or pair-fed control diet and given a placebo or Pro-2. At the end of the 2- and 6-week periods, blood samples were obtained, the animals were sacrificed, and liver tissues were removed. Serum alanine aminotransferase activity was determined; histologic and immunohistochemical analysis was performed for steatosis, inflammation, protein expression of tumor necrosis factor-α, and apoptosis markers. Results: In both studies, methionine choline-deficient diet caused an elevation of serum alanine aminotransferase activity, which was slightly reduced by Pro-1 and Pro-2. In the 2- and 6-week studies, feeding with methionine choline-deficient diet resulted in steatosis and inflammation, but not fibrosis, in all rats. In the 2-week study, in rats fed with methionine choline-deficient diet and given Pro-1, steatosis and inflammation were present in 2 of 6 rats. In rats fed with methionine choline-deficient diet and given Pro-2, steatosis was detected in 3 of 6 rats, while inflammation was present in 2 of 6 rats. In the 6-week study, in rats fed with methionine choline-deficient diet and given Pro-2, steatosis and inflammation were present in 3 of 6 rat livers. In both the 2- and 6-week studies, methionine choline-deficient diet resulted in tumor necrosis factor-α, proapoptotic Bax, caspase 3, caspase 8, and anti- apoptotic Bcl-2 expression in all rat livers. Pro-1 and Pro-2 treatment influenced protein expression involved in apoptosis and tumor necrosis factor-α in varying degrees. Conclusions: Pro-1 and Pro-2 decrease methionine choline-deficient diet-induced steatohepatitis in rats. The preventive effect of probiotics may be due, in part, to modulation of apoptosis and their anti-inflammatory activity.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARAHAN N, İŞLER M, KOYU A, KARAHAN A, BAŞYİĞİT KILIÇ G, ÇİRİŞ İ, SÜTÇÜ R, ONARAN İ, ÇAM H, Keskin M (2012). Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. , 110 - 121.
Chicago	KARAHAN NERMİN,İŞLER Mehmet,KOYU Ahmet,KARAHAN Aynur G.,BAŞYİĞİT KILIÇ GÜLDEN,ÇİRİŞ İbrahim Metin,SÜTÇÜ Recep,ONARAN İBRAHİM,ÇAM Hakan,Keskin Muharrem Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. (2012): 110 - 121.
MLA	KARAHAN NERMİN,İŞLER Mehmet,KOYU Ahmet,KARAHAN Aynur G.,BAŞYİĞİT KILIÇ GÜLDEN,ÇİRİŞ İbrahim Metin,SÜTÇÜ Recep,ONARAN İBRAHİM,ÇAM Hakan,Keskin Muharrem Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. , 2012, ss.110 - 121.
AMA	KARAHAN N,İŞLER M,KOYU A,KARAHAN A,BAŞYİĞİT KILIÇ G,ÇİRİŞ İ,SÜTÇÜ R,ONARAN İ,ÇAM H,Keskin M Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. . 2012; 110 - 121.
Vancouver	KARAHAN N,İŞLER M,KOYU A,KARAHAN A,BAŞYİĞİT KILIÇ G,ÇİRİŞ İ,SÜTÇÜ R,ONARAN İ,ÇAM H,Keskin M Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. . 2012; 110 - 121.
IEEE	KARAHAN N,İŞLER M,KOYU A,KARAHAN A,BAŞYİĞİT KILIÇ G,ÇİRİŞ İ,SÜTÇÜ R,ONARAN İ,ÇAM H,Keskin M "Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats." , ss.110 - 121, 2012.
ISNAD	KARAHAN, NERMİN vd. "Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats". (2012), 110-121.

APA	KARAHAN N, İŞLER M, KOYU A, KARAHAN A, BAŞYİĞİT KILIÇ G, ÇİRİŞ İ, SÜTÇÜ R, ONARAN İ, ÇAM H, Keskin M (2012). Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. Turkish Journal of Gastroenterology, 23(2), 110 - 121.
Chicago	KARAHAN NERMİN,İŞLER Mehmet,KOYU Ahmet,KARAHAN Aynur G.,BAŞYİĞİT KILIÇ GÜLDEN,ÇİRİŞ İbrahim Metin,SÜTÇÜ Recep,ONARAN İBRAHİM,ÇAM Hakan,Keskin Muharrem Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. Turkish Journal of Gastroenterology 23, no.2 (2012): 110 - 121.
MLA	KARAHAN NERMİN,İŞLER Mehmet,KOYU Ahmet,KARAHAN Aynur G.,BAŞYİĞİT KILIÇ GÜLDEN,ÇİRİŞ İbrahim Metin,SÜTÇÜ Recep,ONARAN İBRAHİM,ÇAM Hakan,Keskin Muharrem Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. Turkish Journal of Gastroenterology, vol.23, no.2, 2012, ss.110 - 121.
AMA	KARAHAN N,İŞLER M,KOYU A,KARAHAN A,BAŞYİĞİT KILIÇ G,ÇİRİŞ İ,SÜTÇÜ R,ONARAN İ,ÇAM H,Keskin M Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. Turkish Journal of Gastroenterology. 2012; 23(2): 110 - 121.
Vancouver	KARAHAN N,İŞLER M,KOYU A,KARAHAN A,BAŞYİĞİT KILIÇ G,ÇİRİŞ İ,SÜTÇÜ R,ONARAN İ,ÇAM H,Keskin M Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats. Turkish Journal of Gastroenterology. 2012; 23(2): 110 - 121.
IEEE	KARAHAN N,İŞLER M,KOYU A,KARAHAN A,BAŞYİĞİT KILIÇ G,ÇİRİŞ İ,SÜTÇÜ R,ONARAN İ,ÇAM H,Keskin M "Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats." Turkish Journal of Gastroenterology, 23, ss.110 - 121, 2012.
ISNAD	KARAHAN, NERMİN vd. "Effects of probiotics on methionine cholinedeficient diet-induced steatohepatitis in rats". Turkish Journal of Gastroenterology 23/2 (2012), 110-121.