Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation

CİVELEK, Erdinç; Kabatas, Serdar; AY, Birol; DURUKSU, Gökhan; Karaoz, Erdal; MÜSLÜMAN, Murat; OKCU, Alparslan; SUBASI, Cansu

Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation

Erdal KARAÖZ, (Kocaeli Üniversitesi, Tıp Fakültesi, Kök Hücre Anabilim Dalı, Kocaeli, Türkiye)

Serdar KABATAŞ, (Başkent Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

GÖKHAN DURUKSU, (Kocaeli Üniversitesi, Tıp Fakültesi, Kök Hücre Anabilim Dalı, Kocaeli, Türkiye)

Alparslan OKCU, (Kocaeli Üniversitesi, Tıp Fakültesi, Kök Hücre Anabilim Dalı, Kocaeli, Türkiye)

Cansu SUBASI, (Kocaeli Üniversitesi, Tıp Fakültesi, Kök Hücre Anabilim Dalı, Kocaeli, Türkiye)

Birol AY, (Kocaeli Üniversitesi, Tıp Fakültesi, Kök Hücre Anabilim Dalı, Kocaeli, Türkiye)

Murat MÜSLÜMAN, (Şişli Etfal Eğitim ve Araştırma Hastanesi, Nöroşirürji, Bölümü, İstanbul, Türkiye)

Erdinç CİVELEK (Başkent Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Turkish Neurosurgery

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Yıl: 2012 Cilt: 22 Sayı: 2 Sayfa Aralığı: 207 - 217 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation

Öz:

AMAÇ: Bu çalışmada omurilik hasarı sonrası sıçan kemik iliği-mezenkimal kök hücrelerinin (sKİ-MKH) lezyon yerindeki etkisini analiz etmek ve transplantasyon sonrası işlevsel iyileşmeyi gözlemlemek amaçlandı. YÖNTEM ve GEREÇLER: MKH’ler sıçan femur ve tibialarından izole edildi. Deney sıçanları dört gruba ayrıldı: sadece laminektomi (1), laminektomi+ travma (2); laminektomi + travma + PBS (3); laminektomi + travma + MKH’ler (4). Sıçanların hareketleri düzenli olarak skorlandı. Dört hafta sonra sıçanlar sakrifiye edildi, omurilikleri immün boyamayla GFP işaretli sKİ-MKH’lerle incelendi. BULGULAR: Erken posttravmatik dönemde, Grup 4’teki omurilik dokusu ultrastrüktürel olarak korundu. Santral kanalın etrafındaki ependimal bölgeyi oluşturan hücrelerinin çoğu MKH’ler olarak saptandı. Ependimal bölgenin etrafındaki gri ve ak maddeler astrositik görünümlü Nestin+/ GFAP+ hücrelerden oluşmuştu. Grup 4’te özellikle arka bacak fonksiyonlarında motor iyileşme belirgindi. Buna karşın, diğer gruplarda belirgin değişiklik izlenmedi. SONUÇ: Ependimal bölgenin etrafındaki gri ve ak maddede Nestin+/GFAP+ hücrelerin artışı kendini yenileme ve plastisite potansiyellerini gösterebilir ve sKİ-MKH’lerin transplantasyonu spinal kord travması sonrası fonksiyonel iyileşmede etkili bir strateji olabilir. Sonuç olarak, moleküler faktörler hücre kaderini belirleyen hücre tabanlı tedavinin onarıcı potansiyelini artırmak için uygulanabilir.

Anahtar Kelime:

Konular: Cerrahi

Omurilik hasarlı sıçanlarda kemik iliği kaynaklı mezenkimal kök hücre transplantasyonu sonrası lezyonda azalma ve harekette kısmi işlevsel iyileşme

Öz:

AIm: This study aimed to analyze the effect of rat bone marrow-mesenchymal stem cells (rBM-MSCs) delivery on lesion site after spinal cord injury, and to observe the functional recovery after transplantation. MaterIal and Methods: MSCs were isolated from rat femurs and tibias. The experimental rat population was divided into four groups: only laminectomy (1); laminectomy+trauma (2); laminectomy+trauma+PBS (3); laminectomy+trauma+MSCs (4). Their motility were scored regularly. After 4-weeks, rats were sacrificed, and their spinal cords were examined for GFP labeled rBM-MSCs by immunostainings. Results: In the early posttraumatic period, the ultrastructures of spinal cord tissue were preserved in Group 4. The majority of cells forming the ependymal region around the central canal were found to be MSCs. The gray-and-white-matter around the ependymal region were composed of Nestin+/GFAP+ cells, with astrocytic-like appearance. The scores showed significant motor recovery in Group 4, especially in hind limb functions. However, no obvious change was observed in other groups. ConclusIon: The increase Nestin+/GFAP+ cells in the gray-and-white-matter around the ependymal region could indicate the potential to self-renew and plasticity. Thus, transplantation of rBM-MSCs might be an effective strategy to improve functional recovery following spinal cord trauma. In conclusion, molecular factors in cell fate decisions could be manipulated to enhance reparative potential of cell-based therapy.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Karaoz E, Kabatas S, DURUKSU G, OKCU A, SUBASI C, AY B, MÜSLÜMAN M, CİVELEK E (2012). Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. , 207 - 217.
Chicago	Karaoz Erdal,Kabatas Serdar,DURUKSU Gökhan,OKCU Alparslan,SUBASI Cansu,AY Birol,MÜSLÜMAN Murat,CİVELEK Erdinç Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. (2012): 207 - 217.
MLA	Karaoz Erdal,Kabatas Serdar,DURUKSU Gökhan,OKCU Alparslan,SUBASI Cansu,AY Birol,MÜSLÜMAN Murat,CİVELEK Erdinç Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. , 2012, ss.207 - 217.
AMA	Karaoz E,Kabatas S,DURUKSU G,OKCU A,SUBASI C,AY B,MÜSLÜMAN M,CİVELEK E Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. . 2012; 207 - 217.
Vancouver	Karaoz E,Kabatas S,DURUKSU G,OKCU A,SUBASI C,AY B,MÜSLÜMAN M,CİVELEK E Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. . 2012; 207 - 217.
IEEE	Karaoz E,Kabatas S,DURUKSU G,OKCU A,SUBASI C,AY B,MÜSLÜMAN M,CİVELEK E "Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation." , ss.207 - 217, 2012.
ISNAD	Karaoz, Erdal vd. "Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation". (2012), 207-217.

APA	Karaoz E, Kabatas S, DURUKSU G, OKCU A, SUBASI C, AY B, MÜSLÜMAN M, CİVELEK E (2012). Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. Turkish Neurosurgery, 22(2), 207 - 217.
Chicago	Karaoz Erdal,Kabatas Serdar,DURUKSU Gökhan,OKCU Alparslan,SUBASI Cansu,AY Birol,MÜSLÜMAN Murat,CİVELEK Erdinç Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. Turkish Neurosurgery 22, no.2 (2012): 207 - 217.
MLA	Karaoz Erdal,Kabatas Serdar,DURUKSU Gökhan,OKCU Alparslan,SUBASI Cansu,AY Birol,MÜSLÜMAN Murat,CİVELEK Erdinç Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. Turkish Neurosurgery, vol.22, no.2, 2012, ss.207 - 217.
AMA	Karaoz E,Kabatas S,DURUKSU G,OKCU A,SUBASI C,AY B,MÜSLÜMAN M,CİVELEK E Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. Turkish Neurosurgery. 2012; 22(2): 207 - 217.
Vancouver	Karaoz E,Kabatas S,DURUKSU G,OKCU A,SUBASI C,AY B,MÜSLÜMAN M,CİVELEK E Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. Turkish Neurosurgery. 2012; 22(2): 207 - 217.
IEEE	Karaoz E,Kabatas S,DURUKSU G,OKCU A,SUBASI C,AY B,MÜSLÜMAN M,CİVELEK E "Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation." Turkish Neurosurgery, 22, ss.207 - 217, 2012.
ISNAD	Karaoz, Erdal vd. "Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation". Turkish Neurosurgery 22/2 (2012), 207-217.